##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631458.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2247588
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.120189287360496	34.0	33.0	34.0	31.0	34.0
2	33.25405011950589	34.0	33.0	34.0	31.0	34.0
3	33.34046987259231	34.0	34.0	34.0	31.0	34.0
4	36.55297723604148	37.0	37.0	37.0	35.0	37.0
5	36.53853597723426	37.0	37.0	37.0	35.0	37.0
6	36.58426588858812	37.0	37.0	37.0	35.0	37.0
7	36.59303484446438	37.0	37.0	37.0	35.0	37.0
8	36.58715031402552	37.0	37.0	37.0	35.0	37.0
9	38.442484120755225	39.0	39.0	39.0	37.0	39.0
10	38.421995490276686	39.0	39.0	39.0	37.0	39.0
11	38.46837543179622	39.0	39.0	39.0	37.0	39.0
12	38.43680647876746	39.0	39.0	39.0	37.0	39.0
13	38.45495526760242	39.0	39.0	39.0	37.0	39.0
14	40.030573218935146	41.0	40.0	41.0	38.0	41.0
15	40.01541741635923	41.0	40.0	41.0	38.0	41.0
16	39.997810096868285	41.0	40.0	41.0	38.0	41.0
17	39.99774424850106	41.0	40.0	41.0	38.0	41.0
18	39.98090219381844	41.0	40.0	41.0	38.0	41.0
19	39.990275797877544	41.0	40.0	41.0	38.0	41.0
20	39.956244649820164	41.0	40.0	41.0	38.0	41.0
21	39.93149945630605	41.0	40.0	41.0	38.0	41.0
22	39.88036775423254	41.0	40.0	41.0	38.0	41.0
23	39.83474195448632	41.0	40.0	41.0	38.0	41.0
24	39.810953341982604	41.0	40.0	41.0	38.0	41.0
25	39.80619802205742	41.0	40.0	41.0	38.0	41.0
26	39.72462568762602	41.0	40.0	41.0	37.0	41.0
27	39.68477763718261	41.0	40.0	41.0	37.0	41.0
28	39.62941873688594	41.0	40.0	41.0	37.0	41.0
29	39.59304596749938	41.0	40.0	41.0	37.0	41.0
30	39.540183521179145	41.0	40.0	41.0	37.0	41.0
31	39.49600638551193	41.0	40.0	41.0	36.0	41.0
32	39.43097978811063	41.0	39.0	41.0	36.0	41.0
33	39.3663580691835	41.0	39.0	41.0	36.0	41.0
34	39.31607127284894	41.0	39.0	41.0	35.0	41.0
35	39.24406741804993	41.0	39.0	41.0	35.0	41.0
36	39.19010067681443	41.0	39.0	41.0	35.0	41.0
37	39.14271521293048	41.0	39.0	41.0	35.0	41.0
38	39.08247774948077	41.0	39.0	41.0	35.0	41.0
39	39.0170302564349	41.0	39.0	41.0	35.0	41.0
40	38.935227007796804	41.0	39.0	41.0	35.0	41.0
41	38.85737510611376	41.0	39.0	41.0	35.0	41.0
42	38.73986736003218	40.0	38.0	41.0	35.0	41.0
43	37.98723209057888	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	0.0
13	2.0
14	5.0
15	7.0
16	6.0
17	7.0
18	19.0
19	56.0
20	86.0
21	192.0
22	348.0
23	621.0
24	940.0
25	1659.0
26	2426.0
27	3481.0
28	5060.0
29	7077.0
30	9769.0
31	12887.0
32	16280.0
33	22059.0
34	32786.0
35	49677.0
36	82230.0
37	160061.0
38	383715.0
39	1456127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58267084536846	18.123383823013825	12.797496694233997	26.49644863738372
2	18.59944972121225	19.741562955488284	34.58285059361413	27.076136729685334
3	20.25046405302039	20.674874576657288	28.627177222871808	30.44748414745051
4	15.12154362810266	15.258356958659682	32.845566002310036	36.774533410927624
5	16.933219077517766	34.198349519573874	31.76124805791809	17.107183344990272
6	35.46695390792263	33.42556553959178	15.32015654114544	15.787324011340157
7	30.70527160671796	29.355202109995247	19.90155669099497	20.037969592291827
8	28.091491857048535	31.74006979926926	19.610578095273688	20.557860248408517
9	26.958944432876486	14.412917314027304	19.59985548952922	39.02828276356699
10	19.059943370404184	25.012368814925157	30.45834022961504	25.469347585055623
11	37.58028606666347	20.859027544194042	20.162770045043843	21.397916344098654
12	23.967737859429754	24.49292308020865	26.92691009206314	24.61242896829846
13	31.194418194081834	18.380236947340883	24.30592261571071	26.119422242866573
14	24.406430360012603	19.45138521828734	23.294438304529123	32.84774611717094
15	26.514245493391137	26.397364641562422	21.172786115604815	25.915603749441622
16	26.738619355504657	24.386675849844366	22.64351829605782	26.231186498593157
17	25.246441963562717	25.053257091602198	23.608597305200064	26.09170363963502
18	26.325331866872396	22.97818817327731	24.82648065392768	25.869999305922615
19	25.979805907488384	24.00546719416548	24.1643486261717	25.85037827217444
20	26.2152138203265	23.700473574338357	24.202389405887555	25.881923199447588
21	27.707569180828518	23.56503950012191	24.388811472565255	24.338579846484322
22	27.23617495733204	23.022012931195576	23.74007157895486	26.001740532517527
23	25.88735124052985	23.455455359256234	24.30316410302956	26.35402929718436
24	25.559844597853342	23.93023098539412	23.968271765109975	26.54165265164256
25	26.221976625609322	23.836263585674956	23.982019836375706	25.959739952340023
26	26.132102502771858	24.001596377983866	24.118877659072748	25.747423460171525
27	26.471310578273243	23.122253722657355	24.230508438379275	26.175927260690123
28	25.37190979841501	23.337106266806906	24.895532455236456	26.395451479541627
29	24.90621056884091	23.558855092659332	24.922850629207844	26.612083709291916
30	24.592407505290115	24.33728957442378	25.32732867411643	25.74297424616967
31	25.918184293562703	23.671242238346174	24.2204977068751	26.190075761216026
32	25.542136726125964	23.317040311658545	24.499062995531208	26.641759966684287
33	25.084223621055102	22.78949700745866	25.36794999795336	26.758329373532874
34	25.91240031536029	22.4968722025567	25.109406172305597	26.481321309777417
35	25.327150705556356	22.666565224587423	26.016511923003684	25.989772146852534
36	24.336310747343372	22.57255333272824	26.502544060566258	26.588591859362126
37	25.404567029188623	22.051906310231235	26.26139666166575	26.282129998914392
38	24.22125407325542	21.637995931638716	27.588374737718834	26.552375257387027
39	24.503512209533064	21.54887817518157	27.490981443218242	26.45662817206712
40	23.603169264117803	22.49126619291436	27.747167185445022	26.158397357522823
41	23.360108703196495	22.130746382344096	28.62339539097023	25.88574952348918
42	23.720495037346705	22.391158877872634	28.08508498888586	25.803261095894797
43	22.27031822558227	21.9661699564155	28.306833814738287	27.456678003263946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	395.0
1	386.0
2	377.0
3	775.0
4	1173.0
5	1173.0
6	1356.0
7	1539.0
8	1465.5
9	1392.0
10	1839.5
11	2287.0
12	2287.0
13	3529.0
14	4771.0
15	7101.5
16	9432.0
17	8914.0
18	8396.0
19	8396.0
20	9864.5
21	11333.0
22	10403.0
23	9473.0
24	10723.0
25	11973.0
26	11973.0
27	14274.0
28	16575.0
29	20191.5
30	23808.0
31	27758.0
32	31708.0
33	31708.0
34	40161.0
35	48614.0
36	61758.0
37	74902.0
38	83934.0
39	92966.0
40	92966.0
41	102339.5
42	111713.0
43	123414.0
44	135115.0
45	146290.0
46	157465.0
47	157465.0
48	165741.5
49	174018.0
50	179485.0
51	184952.0
52	189225.0
53	193498.0
54	193498.0
55	185177.0
56	176856.0
57	168728.0
58	160600.0
59	156006.0
60	151412.0
61	151412.0
62	144731.0
63	138050.0
64	121131.0
65	104212.0
66	90887.5
67	77563.0
68	77563.0
69	62908.5
70	48254.0
71	41132.5
72	34011.0
73	25516.0
74	17021.0
75	17021.0
76	13294.0
77	9567.0
78	8078.5
79	6590.0
80	6115.0
81	5640.0
82	5640.0
83	4600.5
84	3561.0
85	3248.5
86	2936.0
87	2569.0
88	2202.0
89	2202.0
90	1571.0
91	940.0
92	568.5
93	197.0
94	130.5
95	64.0
96	64.0
97	46.0
98	28.0
99	18.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2247588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.996894495358795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0702766884649	35.30690540281083
2	7.744593473815497	6.504977500585474
3	2.5482728288659526	3.2105863541781874
4	1.2827283366767002	2.154824264864738
5	0.8205798207645002	1.7230902078833572
6	0.5528412528690317	1.393056946165362
7	0.40639547495699296	1.194714351961204
8	0.30980152166378055	1.040856145585232
9	0.23536936843608336	0.8896304280284482
>10	1.585355307671304	13.082806017278937
>50	0.2161460517360392	6.3999292041226274
>100	0.20100376343552767	17.241140778072445
>500	0.020162792917591484	5.684220204749902
>1k	0.006473317726174109	4.1732621937133425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4943	0.2199246481116646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3990	0.17752363867399185	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3697	0.16448744164855836	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3120	0.13881547685785828	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2459	0.1094061723056005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.4492140018544326E-5	0.0
3	0.0	0.0	0.0	8.898428003708865E-5	0.0
4	0.0	0.0	0.0	2.2246070009272162E-4	0.0
5	0.0	0.0	0.0	3.114449801298103E-4	0.0
6	0.0	0.0	0.0	3.114449801298103E-4	0.0
7	0.0	0.0	0.0	4.4492140018544325E-4	0.0
8	0.0	0.0	0.0	4.894135402039875E-4	0.0
9	0.0	0.0	0.0	0.0011567956404821525	0.0
10	0.0	0.0	0.0	0.0015572249006490513	0.0
11	0.0	0.0	0.0	0.002180114860908672	0.0
12	0.0	0.0	0.0	0.002847496961186837	0.0
13	0.0	0.0	0.0	0.0031144498012981026	0.0
14	0.0	0.0	0.0	0.00360386334150209	0.0
15	0.0	0.0	0.0	0.0042712454417802555	0.0
16	0.0	0.0	0.0	0.005783978202410762	0.0
17	0.0	0.0	0.0	0.008008585203337979	0.0
18	0.0	4.4492140018544326E-5	0.0	0.009298857263875764	0.0
19	0.0	4.4492140018544326E-5	0.0	0.01027768434428374	0.0
20	0.0	4.4492140018544326E-5	0.0	0.012012877805006967	4.4492140018544326E-5
21	0.0	4.4492140018544326E-5	0.0	0.01450443764604545	8.898428003708865E-5
22	0.0	4.4492140018544326E-5	0.0	0.018642206667770073	8.898428003708865E-5
23	0.0	4.4492140018544326E-5	0.0	0.024025755610013935	8.898428003708865E-5
24	0.0	4.4492140018544326E-5	0.0	0.03323562859385261	8.898428003708865E-5
25	0.0	4.4492140018544326E-5	0.0	0.03928655963637464	8.898428003708865E-5
26	0.0	4.4492140018544326E-5	0.0	0.05187783526162268	8.898428003708865E-5
27	0.0	4.4492140018544326E-5	0.0	0.11100788934626808	8.898428003708865E-5
28	0.0	4.4492140018544326E-5	0.0	0.3489073620254246	8.898428003708865E-5
29	0.0	4.4492140018544326E-5	0.0	0.6774373239223559	8.898428003708865E-5
30	0.0	4.4492140018544326E-5	0.0	1.135439413273251	8.898428003708865E-5
31	0.0	4.4492140018544326E-5	0.0	1.9694890700608831	8.898428003708865E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2240	0.0	24.11607	1
CTAGACA	665	0.0	21.142857	4
AACCGAT	45	0.0038269297	20.555555	7
ACTGATC	575	0.0	20.269566	8
TCTAGAC	670	0.0	19.880596	3
CTAGAAC	740	0.0	19.75	3
TAGAACT	810	0.0	19.41358	4
GTCTAGA	745	0.0	19.369127	1
ATACACA	1160	0.0	18.818966	37
GTTCTAG	680	0.0	18.772058	1
TATGCGG	70	1.21985555E-4	18.5	5
AATTCGA	50	0.0070369234	18.5	24
ACATGTA	535	0.0	17.28972	8
GCAGTCG	400	0.0	17.112501	9
CTCTAGT	725	0.0	16.586206	27
GTATCAA	3250	0.0	16.507692	2
ACGGAGT	640	0.0	16.476562	26
AGTACTA	90	4.449E-5	16.444445	5
ACAACGG	655	0.0	16.38168	23
AACGGAG	665	0.0	16.135338	25
>>END_MODULE