FastQCFastQC Report
Fri 10 Feb 2017
ERR1631457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343314
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.29885178000314583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8840.2574902276050496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7180.2091379902945991No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3970.11563757959186052No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3800.11068584444560957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC352.383467E-531.7142853
ATCGTAC358.8566775E-426.4285725
TAATACC400.001928444823.1254
CGTACTA400.001928444823.12527
CATCGTA400.001928444823.12524
AATACCC400.001928444823.1255
TACACGA400.001928444823.12532
GGTATCA5200.022.4134621
CGTCGTA502.6967571E-422.19999910
GATCACG603.7164962E-521.58333223
ATCACGT603.7164962E-521.58333224
ATACCGC705.084743E-621.14285727
GACACGT450.00382042620.55555537
TGTATAT450.00382042620.5555553
CCGTCGT450.00382042620.5555559
ATCGGAG450.00382042620.55555518
AATCGGA450.00382042620.55555517
GCAGTCG2550.020.3137259
AGTCGGT2650.020.24528311
TAAAGTA555.133001E-420.18181826
Produced by FastQC (version 0.11.5)