##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631457.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343314
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.068313555520604	34.0	33.0	34.0	31.0	34.0
2	33.21032058115894	34.0	33.0	34.0	31.0	34.0
3	33.29370488823643	34.0	34.0	34.0	31.0	34.0
4	36.51545232644168	37.0	37.0	37.0	35.0	37.0
5	36.50876165842348	37.0	37.0	37.0	35.0	37.0
6	36.55605655464094	37.0	37.0	37.0	35.0	37.0
7	36.56402302265565	37.0	37.0	37.0	35.0	37.0
8	36.55982569892285	37.0	37.0	37.0	35.0	37.0
9	38.41391262808974	39.0	39.0	39.0	37.0	39.0
10	38.371036427293966	39.0	39.0	39.0	37.0	39.0
11	38.43074270201623	39.0	39.0	39.0	37.0	39.0
12	38.38819273318303	39.0	39.0	39.0	37.0	39.0
13	38.41722155228158	39.0	39.0	39.0	37.0	39.0
14	39.971868318798535	41.0	40.0	41.0	38.0	41.0
15	39.96177260467094	41.0	40.0	41.0	38.0	41.0
16	39.92773670750392	41.0	40.0	41.0	38.0	41.0
17	39.916152560047074	41.0	40.0	41.0	38.0	41.0
18	39.89831466237904	41.0	40.0	41.0	38.0	41.0
19	39.92321023902317	41.0	40.0	41.0	38.0	41.0
20	39.89211334230471	41.0	40.0	41.0	38.0	41.0
21	39.86396127160558	41.0	40.0	41.0	38.0	41.0
22	39.808830982715534	41.0	40.0	41.0	38.0	41.0
23	39.76147491800509	41.0	40.0	41.0	38.0	41.0
24	39.74832369201373	41.0	40.0	41.0	38.0	41.0
25	39.73050618384336	41.0	40.0	41.0	37.0	41.0
26	39.63235988045929	41.0	40.0	41.0	37.0	41.0
27	39.58542034405821	41.0	40.0	41.0	37.0	41.0
28	39.52509073326459	41.0	40.0	41.0	37.0	41.0
29	39.48297768223842	41.0	40.0	41.0	37.0	41.0
30	39.41184455046983	41.0	39.0	41.0	36.0	41.0
31	39.34926918214812	41.0	39.0	41.0	36.0	41.0
32	39.27987498325148	41.0	39.0	41.0	35.0	41.0
33	39.19716644238219	41.0	39.0	41.0	35.0	41.0
34	39.15060265529515	41.0	39.0	41.0	35.0	41.0
35	39.0671397030124	41.0	39.0	41.0	35.0	41.0
36	39.01230069266036	41.0	39.0	41.0	35.0	41.0
37	38.94128698509236	41.0	39.0	41.0	35.0	41.0
38	38.8620067926155	41.0	39.0	41.0	35.0	41.0
39	38.76460616228875	40.0	38.0	41.0	35.0	41.0
40	38.67520986618664	40.0	38.0	41.0	35.0	41.0
41	38.57929475640376	40.0	38.0	41.0	35.0	41.0
42	38.44344827184443	40.0	38.0	41.0	35.0	41.0
43	37.64624804115183	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	3.0
18	1.0
19	5.0
20	13.0
21	35.0
22	64.0
23	92.0
24	168.0
25	258.0
26	417.0
27	625.0
28	857.0
29	1151.0
30	1698.0
31	2169.0
32	2851.0
33	3670.0
34	5744.0
35	8634.0
36	13634.0
37	27821.0
38	65167.0
39	208235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1278246736224	16.926487122575836	11.631625858543492	27.31406234525828
2	19.666835608218715	19.126513920201333	32.833208083561985	28.373442388017967
3	20.102005744012768	21.23653564958027	28.313439009186926	30.348019597220038
4	14.379838864712772	14.99035868039171	33.80869990737342	36.821102547522095
5	16.06663287835626	35.14071666171493	32.675335115957985	16.117315343970827
6	35.18848634194936	33.8436533319352	15.877301828646662	15.09055849746879
7	32.059863565132794	28.677537181705375	19.80606674938977	19.456532503772056
8	30.227139003943908	30.6116266741234	18.550364972008133	20.61086934992456
9	28.15964394111513	13.322497771719183	18.069464105745762	40.44839418141992
10	18.839895838794806	24.706245594412113	30.428703752250126	26.025154814542955
11	38.44439783987836	20.631258847585592	19.556149763773107	21.36819354876294
12	23.7884269211276	24.296416691425343	26.80723768911259	25.10791869833447
13	32.302207308761076	17.598466709775888	23.276359251297645	26.822966730165387
14	24.09019148651089	18.792708715636415	23.350343999953395	33.7667557978993
15	27.8631223894161	25.375021117694008	20.283472273195965	26.478384219693922
16	27.22260088432164	23.877849432298127	22.404562587019463	26.494987096360767
17	25.691058331440026	24.75255888195646	23.28946678550831	26.266916001095208
18	25.956121801033454	22.87352103322323	24.68585609675108	26.484501068992234
19	26.638004858526017	23.695217788962875	24.362245641016678	25.304531711494434
20	26.660724584491163	22.846432129187857	24.33282650867719	26.16001677764379
21	27.644080928829002	23.028481215447083	23.777067058145022	25.550370797578893
22	27.758262115730787	22.58049482398038	23.3957834518837	26.265459608405134
23	26.316433352557716	22.872355919071172	24.02348870130551	26.787722027065602
24	25.87310741769925	23.336945187204716	23.93901792528123	26.850929469814805
25	26.34235714244103	22.757592175093354	24.0765013952242	26.823549287241416
26	26.362746640102063	23.53530587159277	23.784349021595393	26.317598466709775
27	26.410516320336484	23.02382075883885	23.5813278805991	26.984335040225567
28	25.725429198925763	22.993527790885313	24.7947942699686	26.486248740220326
29	25.532020249683963	23.30344815533302	25.017331073011878	26.147200521971143
30	25.04092463459107	24.12019317592641	24.76217107371095	26.076711115771566
31	26.056321618110534	23.782310071829286	24.008342217328742	26.153026092731434
32	25.521242943777416	23.394327059193625	24.39370372312227	26.690726273906684
33	25.121900068159174	23.22829829252521	24.883925502601116	26.765876136714496
34	26.3318711150725	22.848179800415945	24.365158426396828	26.454790658114728
35	25.81164764617813	23.6247283827633	24.820718059851917	25.74290591120665
36	25.192680752896766	23.894743587502987	24.329039887682995	26.583535771917255
37	25.670086276702957	22.830994366673075	24.94771550242635	26.551203854197613
38	24.80528029733713	22.754388111175192	25.789510477289014	26.650821114198664
39	25.239285318979128	22.548454184798754	25.745527418048788	26.466733078173334
40	24.754597831722563	22.207949573859498	26.563437552794234	26.474015041623705
41	23.79483504896392	22.39640678795505	27.150946363970007	26.65781179911102
42	24.005429431948595	22.24173788426921	27.512714308184343	26.24011837559785
43	23.270242401999337	21.761710853620883	27.95108850789656	27.01695823648322
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	15.0
2	20.0
3	59.0
4	98.0
5	98.0
6	126.0
7	154.0
8	141.5
9	129.0
10	210.5
11	292.0
12	292.0
13	530.0
14	768.0
15	1285.0
16	1802.0
17	1623.0
18	1444.0
19	1444.0
20	1684.5
21	1925.0
22	1517.5
23	1110.0
24	1237.0
25	1364.0
26	1364.0
27	1646.5
28	1929.0
29	2622.5
30	3316.0
31	4039.0
32	4762.0
33	4762.0
34	6086.5
35	7411.0
36	9108.5
37	10806.0
38	12436.5
39	14067.0
40	14067.0
41	15634.0
42	17201.0
43	18807.5
44	20414.0
45	21714.0
46	23014.0
47	23014.0
48	25055.5
49	27097.0
50	27362.5
51	27628.0
52	27287.5
53	26947.0
54	26947.0
55	26251.0
56	25555.0
57	24482.5
58	23410.0
59	22850.0
60	22290.0
61	22290.0
62	20913.5
63	19537.0
64	18231.5
65	16926.0
66	15161.5
67	13397.0
68	13397.0
69	11591.0
70	9785.0
71	8401.5
72	7018.0
73	5551.5
74	4085.0
75	4085.0
76	3412.0
77	2739.0
78	2244.0
79	1749.0
80	1531.5
81	1314.0
82	1314.0
83	1043.5
84	773.0
85	647.0
86	521.0
87	418.0
88	315.0
89	315.0
90	238.0
91	161.0
92	89.0
93	17.0
94	13.5
95	10.0
96	10.0
97	6.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	343314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.83297271995138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4670548605632	55.83282126808822
2	6.619792265197461	8.45122038152189
3	1.9487265314195694	3.731790225562525
4	0.9800021648370685	2.502258058141515
5	0.576020534080282	1.8384551519039234
6	0.4069631580987844	1.5586600901366978
7	0.29487850901753515	1.3176080275271382
8	0.22692901573278587	1.1588442936509071
9	0.1877607494208321	1.0786794116091842
>10	1.1527971859503685	14.047785195615667
>50	0.10430626926157813	4.467696675318307
>100	0.03339630550918366	3.2467384558586945
>500	9.1496727422421E-4	0.4678246992521702
>1k	4.57483637112105E-4	0.2996180658131876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1026	0.29885178000314583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.2574902276050496	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	718	0.2091379902945991	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	397	0.11563757959186052	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	380	0.11068584444560957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.91278538014762E-4	0.0
13	0.0	0.0	0.0	2.91278538014762E-4	0.0
14	0.0	0.0	0.0	2.91278538014762E-4	0.0
15	0.0	0.0	0.0	2.91278538014762E-4	0.0
16	0.0	0.0	0.0	8.73835614044286E-4	0.0
17	0.0	0.0	0.0	0.0014563926900738099	0.0
18	0.0	0.0	0.0	0.0014563926900738099	0.0
19	0.0	0.0	0.0	0.001747671228088572	0.0
20	0.0	0.0	0.0	0.001747671228088572	0.0
21	0.0	0.0	0.0	0.002038949766103334	0.0
22	0.0	0.0	0.0	0.003495342456177144	0.0
23	0.0	0.0	0.0	0.006408127836324764	0.0
24	0.0	0.0	0.0	0.008155799064413336	0.0
25	0.0	0.0	0.0	0.010486027368531431	0.0
26	0.0	0.0	0.0	0.015146483976767624	0.0
27	0.0	0.0	0.0	0.03320575333368287	0.0
28	0.0	0.0	0.0	0.08767483994244336	0.0
29	0.0	0.0	0.0	0.16835899497253243	0.0
30	0.0	0.0	0.0	0.27846228234211245	0.0
31	0.0	0.0	0.0	0.553137943690033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATAC	35	2.383467E-5	31.714285	3
ATCGTAC	35	8.8566775E-4	26.42857	25
TAATACC	40	0.0019284448	23.125	4
CGTACTA	40	0.0019284448	23.125	27
CATCGTA	40	0.0019284448	23.125	24
AATACCC	40	0.0019284448	23.125	5
TACACGA	40	0.0019284448	23.125	32
GGTATCA	520	0.0	22.413462	1
CGTCGTA	50	2.6967571E-4	22.199999	10
GATCACG	60	3.7164962E-5	21.583332	23
ATCACGT	60	3.7164962E-5	21.583332	24
ATACCGC	70	5.084743E-6	21.142857	27
GACACGT	45	0.003820426	20.555555	37
TGTATAT	45	0.003820426	20.555555	3
CCGTCGT	45	0.003820426	20.555555	9
ATCGGAG	45	0.003820426	20.555555	18
AATCGGA	45	0.003820426	20.555555	17
GCAGTCG	255	0.0	20.313725	9
AGTCGGT	265	0.0	20.245283	11
TAAAGTA	55	5.133001E-4	20.181818	26
>>END_MODULE