FastQCFastQC Report
Fri 10 Feb 2017
ERR1631456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186282
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT352.3776554E-531.7142872
AGTCGCT303.5880096E-430.83333422
GGCGACG250.00548467229.612
ATTCGTA405.9138478E-527.7524
TTCGTAT405.9138478E-527.7525
GCGTTAT405.9138478E-527.751
TTTGCGC405.9138478E-527.7517
TTGCGCG509.037796E-625.90000218
ATACACA2400.024.66666837
AATTTGC451.317405E-424.66666615
GTTATTC451.317405E-424.6666663
TAAGAGG551.8915842E-523.5454544
TGACCCG400.001925149923.12513
TAGATAA400.001925149923.1254
GTGGGTA502.6903398E-422.29
ATTTGCG502.6903398E-422.216
GTCGGGA502.6903398E-422.22
TGCGCGC603.7047506E-521.58333419
TTAAGAG806.895898E-720.81253
TTAGGAC450.003813962220.5555553
Produced by FastQC (version 0.11.5)