##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631456.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186282
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.065390107471465	34.0	33.0	34.0	31.0	34.0
2	33.20966062206762	34.0	33.0	34.0	31.0	34.0
3	33.300716118572915	34.0	34.0	34.0	31.0	34.0
4	36.531334213718985	37.0	37.0	37.0	35.0	37.0
5	36.514633727359595	37.0	37.0	37.0	35.0	37.0
6	36.563253561804146	37.0	37.0	37.0	35.0	37.0
7	36.5619866653783	37.0	37.0	37.0	35.0	37.0
8	36.55684392480218	37.0	37.0	37.0	35.0	37.0
9	38.414613328179854	39.0	39.0	39.0	37.0	39.0
10	38.36816761683899	39.0	39.0	39.0	37.0	39.0
11	38.43659076024522	39.0	39.0	39.0	37.0	39.0
12	38.384427910372445	39.0	39.0	39.0	37.0	39.0
13	38.40551958858075	39.0	39.0	39.0	37.0	39.0
14	39.96907913808097	41.0	40.0	41.0	38.0	41.0
15	39.96526234418784	41.0	40.0	41.0	38.0	41.0
16	39.94122352132788	41.0	40.0	41.0	38.0	41.0
17	39.912186899432044	41.0	40.0	41.0	38.0	41.0
18	39.8875629422059	41.0	40.0	41.0	38.0	41.0
19	39.89989907774235	41.0	40.0	41.0	38.0	41.0
20	39.86753953683126	41.0	40.0	41.0	38.0	41.0
21	39.82874888609742	41.0	40.0	41.0	38.0	41.0
22	39.79122513178944	41.0	40.0	41.0	38.0	41.0
23	39.74094115373466	41.0	40.0	41.0	38.0	41.0
24	39.712924490825735	41.0	40.0	41.0	37.0	41.0
25	39.678739760148595	41.0	40.0	41.0	37.0	41.0
26	39.60882425569835	41.0	40.0	41.0	37.0	41.0
27	39.541383493842666	41.0	40.0	41.0	37.0	41.0
28	39.483294145435416	41.0	39.0	41.0	37.0	41.0
29	39.44382173264191	41.0	39.0	41.0	36.0	41.0
30	39.363121503956364	41.0	39.0	41.0	36.0	41.0
31	39.325093138360124	41.0	39.0	41.0	36.0	41.0
32	39.276392780837654	41.0	39.0	41.0	35.0	41.0
33	39.21923213192901	41.0	39.0	41.0	35.0	41.0
34	39.16321491072675	41.0	39.0	41.0	35.0	41.0
35	39.09620360528661	41.0	39.0	41.0	35.0	41.0
36	39.05371962937912	41.0	39.0	41.0	35.0	41.0
37	39.01431700325313	41.0	39.0	41.0	35.0	41.0
38	38.9599585574559	41.0	39.0	41.0	35.0	41.0
39	38.90139680699155	40.0	39.0	41.0	35.0	41.0
40	38.832608625632105	40.0	38.0	41.0	35.0	41.0
41	38.77202306180951	40.0	38.0	41.0	35.0	41.0
42	38.68808043718663	40.0	38.0	41.0	35.0	41.0
43	37.854113655640376	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	2.0
19	5.0
20	4.0
21	18.0
22	38.0
23	50.0
24	108.0
25	157.0
26	225.0
27	307.0
28	420.0
29	574.0
30	792.0
31	1027.0
32	1340.0
33	1906.0
34	3002.0
35	4765.0
36	7975.0
37	14518.0
38	36040.0
39	113006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11913120967136	16.0863636851655	11.971097583234021	27.823407521929116
2	21.2060209789459	18.533728433235634	30.67070355697276	29.589547030845708
3	21.002566002082865	19.306749981211283	27.484673774170343	32.20601024253551
4	16.10032101867062	14.816783156719382	31.16511525536552	37.91778056924448
5	18.419385662597566	32.34397311602839	31.16887299900151	18.067768222372532
6	37.72184107965343	31.596182132465835	14.619770026089476	16.062206761791263
7	32.88777230220848	27.187812026926917	18.92399695085945	21.000418720005154
8	29.970689599639254	29.951364060939866	18.413480636883865	21.664465702537015
9	27.941508036203178	13.809707862273326	17.402647598801817	40.84613650272168
10	19.924630399072374	23.058051770970895	29.40863851579863	27.608679314158103
11	39.658152693228544	19.462427931845266	18.856894385931007	22.02252498899518
12	25.266531387895768	22.36233237779281	25.62888523850936	26.742250995802063
13	33.0713649198527	16.54588204979547	22.190549811575995	28.192203218775834
14	25.460860415928536	17.744602269677156	21.388003135031834	35.40653417936247
15	28.2480325527963	24.030233731654157	19.41196680301908	28.309766912530463
16	28.819209585467192	22.800914742165105	20.787300973792423	27.592574698575277
17	27.683297366358534	23.048389001621196	21.807796781224166	27.460516850796107
18	27.965664959577413	21.564617085923494	22.61410120140432	27.85561675309477
19	28.30386188681676	22.400983455191593	21.91516088510967	27.37999377288197
20	28.510000966276934	21.635477394487925	21.642456061240484	28.212065577994654
21	29.48647749111562	22.00910447600949	21.313385082831406	27.191032950043482
22	29.74790908407683	21.66070795890102	20.82273112807464	27.76865182894751
23	28.387605887847457	21.57589031683147	21.68915944643068	28.347344348890392
24	27.691886494669372	22.134720477555533	21.716537292921483	28.456855734853608
25	27.748252649209267	21.281175851665754	22.11324765677843	28.85732384234655
26	27.647330391556885	22.458960071289766	22.304892582214062	27.58881695493929
27	28.120806089691975	21.6934540105861	22.095532579637325	28.090207320084602
28	27.494873364039467	22.031650937825447	22.274293812606693	28.19918188552839
29	26.935506382795975	21.95488560354731	23.155753105506705	27.95385490815001
30	26.765871098656874	22.287177505072954	22.942635359293973	28.004316036976196
31	27.19586433471833	21.355257083346753	22.480969712586294	28.967908869348623
32	25.894074575106558	20.863529487551133	23.820336908557994	29.422059028784314
33	26.118465552227267	20.256922300598017	24.79896071547439	28.82565143170033
34	26.666022482043356	19.504836752880042	25.57842410968317	28.250716655393436
35	25.675051803180125	19.1355042355139	26.587646686206934	28.601797275099045
36	24.5847693282228	18.82092741112936	27.357447311066018	29.23685594958182
37	23.928237832962925	17.787011090711932	28.410152349663413	29.874598726661727
38	22.82829258865591	17.112764518310946	30.53166704244103	29.527275850592112
39	22.437487250512664	17.134774159607478	32.01704941969702	28.41068917018284
40	21.619372778905102	18.161711813272348	32.15447547267047	28.06443993515208
41	20.2155871206021	18.335104841047446	33.57114482343973	27.878163214910728
42	19.37277890510087	19.207975005636616	33.261399383730044	28.157846705532474
43	17.893301553558583	19.10490546590653	33.13632020270343	29.86547277783146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	4.5
2	4.0
3	8.0
4	12.0
5	12.0
6	11.5
7	11.0
8	12.0
9	13.0
10	24.0
11	35.0
12	35.0
13	50.0
14	65.0
15	97.5
16	130.0
17	133.0
18	136.0
19	136.0
20	164.5
21	193.0
22	223.0
23	253.0
24	322.5
25	392.0
26	392.0
27	538.5
28	685.0
29	945.5
30	1206.0
31	1508.5
32	1811.0
33	1811.0
34	2305.5
35	2800.0
36	3482.0
37	4164.0
38	5100.0
39	6036.0
40	6036.0
41	6850.5
42	7665.0
43	8672.5
44	9680.0
45	10482.0
46	11284.0
47	11284.0
48	12231.5
49	13179.0
50	13919.0
51	14659.0
52	15584.0
53	16509.0
54	16509.0
55	16289.5
56	16070.0
57	15795.0
58	15520.0
59	15274.0
60	15028.0
61	15028.0
62	13880.0
63	12732.0
64	11689.5
65	10647.0
66	9512.0
67	8377.0
68	8377.0
69	7149.5
70	5922.0
71	5082.5
72	4243.0
73	3315.0
74	2387.0
75	2387.0
76	2007.5
77	1628.0
78	1389.5
79	1151.0
80	950.5
81	750.0
82	750.0
83	566.5
84	383.0
85	317.0
86	251.0
87	203.5
88	156.0
89	156.0
90	117.0
91	78.0
92	50.5
93	23.0
94	14.0
95	5.0
96	5.0
97	4.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	186282.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.48666507232595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.39215723315779	66.58523675918924
2	6.399136022985232	9.533006033927135
3	1.5107352268798235	3.375888865726853
4	0.7325639900108888	2.182649942719512
5	0.4406057996646289	1.6409628314271778
6	0.2869829796693108	1.282584305285165
7	0.18048105990954214	0.9410402582966321
8	0.14867465143457145	0.8859423172921382
9	0.12387356895131371	0.830423613761116
>10	0.7382176338081876	10.251979874672843
>50	0.04062649307823031	1.931557902307826
>100	0.005945340450472728	0.558727295394354
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.368205194275346E-4	0.0
10	0.0	0.0	0.0	0.0010736410388550693	0.0
11	0.0	0.0	0.0	0.0010736410388550693	0.0
12	0.0	0.0	0.0	0.0010736410388550693	0.0
13	0.0	0.0	0.0	0.0010736410388550693	0.0
14	0.0	0.0	0.0	0.0010736410388550693	0.0
15	0.0	0.0	0.0	0.0010736410388550693	0.0
16	0.0	0.0	0.0	0.0010736410388550693	5.368205194275346E-4
17	0.0	0.0	0.0	0.0010736410388550693	5.368205194275346E-4
18	0.0	0.0	0.0	0.0016104615582826038	5.368205194275346E-4
19	0.0	0.0	0.0	0.0021472820777101386	5.368205194275346E-4
20	0.0	0.0	0.0	0.0032209231165652077	5.368205194275346E-4
21	0.0	0.0	0.0	0.0048313846748478115	5.368205194275346E-4
22	0.0	0.0	0.0	0.00697866675255795	5.368205194275346E-4
23	0.0	0.0	0.0	0.01288369246626083	5.368205194275346E-4
24	0.0	0.0	0.0	0.02684102597137673	5.368205194275346E-4
25	0.0	0.0	0.0	0.04026153895706509	5.368205194275346E-4
26	0.0	0.0	0.0	0.06763938544786936	5.368205194275346E-4
27	0.0	0.0	0.0	0.27109436231090495	5.368205194275346E-4
28	0.0	0.0	0.0	1.056462782233388	5.368205194275346E-4
29	0.0	5.368205194275346E-4	0.0	2.0227397172029504	5.368205194275346E-4
30	0.0	5.368205194275346E-4	0.0	3.25957419396399	5.368205194275346E-4
31	0.0	5.368205194275346E-4	0.0	5.76813648124886	5.368205194275346E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	35	2.3776554E-5	31.714287	2
AGTCGCT	30	3.5880096E-4	30.833334	22
GGCGACG	25	0.005484672	29.6	12
ATTCGTA	40	5.9138478E-5	27.75	24
TTCGTAT	40	5.9138478E-5	27.75	25
GCGTTAT	40	5.9138478E-5	27.75	1
TTTGCGC	40	5.9138478E-5	27.75	17
TTGCGCG	50	9.037796E-6	25.900002	18
ATACACA	240	0.0	24.666668	37
AATTTGC	45	1.317405E-4	24.666666	15
GTTATTC	45	1.317405E-4	24.666666	3
TAAGAGG	55	1.8915842E-5	23.545454	4
TGACCCG	40	0.0019251499	23.125	13
TAGATAA	40	0.0019251499	23.125	4
GTGGGTA	50	2.6903398E-4	22.2	9
ATTTGCG	50	2.6903398E-4	22.2	16
GTCGGGA	50	2.6903398E-4	22.2	2
TGCGCGC	60	3.7047506E-5	21.583334	19
TTAAGAG	80	6.895898E-7	20.8125	3
TTAGGAC	45	0.0038139622	20.555555	3
>>END_MODULE