FastQCFastQC Report
Fri 10 Feb 2017
ERR1631455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024899
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT163950.8096700131710273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113980.5628922726516236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111590.5510892148200972No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.2429750817201253No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA23310.11511685274179106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA11850.018.73417737
AATCGTA500.007036688318.518
AGTCGGT15450.017.48220311
GGTATCA69100.017.3219971
GCAGTCG16000.016.9968769
GGCAGTC16400.016.5823158
TCGGTGA16600.016.3825313
CAGTCGG16550.016.32024210
CGGTGAT16450.016.08206714
GGGCAGT17350.015.8876097
TACCTTA700.002593408315.85714231
GATACAC2003.6379788E-1215.7253
GTATAGA1659.749783E-1015.6969691
CGCCTTA12500.015.68825
ATACACT2950.015.6779664
CTTATAC54850.015.61622737
GTCGGTG17550.015.17948712
ATTAGAC1001.0940172E-414.83
CGCTTAT4250.014.826
TTATACA32800.014.77743837
Produced by FastQC (version 0.11.5)