##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631455.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2024899
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.103629366205425	34.0	33.0	34.0	31.0	34.0
2	33.23116461611172	34.0	33.0	34.0	31.0	34.0
3	33.300859944125605	34.0	34.0	34.0	31.0	34.0
4	36.5337732894332	37.0	37.0	37.0	35.0	37.0
5	36.530733137800944	37.0	37.0	37.0	35.0	37.0
6	36.590369198661264	37.0	37.0	37.0	35.0	37.0
7	36.588941966982055	37.0	37.0	37.0	35.0	37.0
8	36.58899925378994	37.0	37.0	37.0	35.0	37.0
9	38.43694525010877	39.0	39.0	39.0	37.0	39.0
10	38.40488340406114	39.0	39.0	39.0	37.0	39.0
11	38.46015480278275	39.0	39.0	39.0	37.0	39.0
12	38.4229094883251	39.0	39.0	39.0	37.0	39.0
13	38.447792210870766	39.0	39.0	39.0	37.0	39.0
14	39.99008592527331	41.0	40.0	41.0	38.0	41.0
15	39.97825175477888	41.0	40.0	41.0	38.0	41.0
16	39.956280288547724	41.0	40.0	41.0	38.0	41.0
17	39.95516615890472	41.0	40.0	41.0	38.0	41.0
18	39.96221441168177	41.0	40.0	41.0	38.0	41.0
19	39.98899204355378	41.0	40.0	41.0	38.0	41.0
20	39.9715432720348	41.0	40.0	41.0	38.0	41.0
21	39.94512269500849	41.0	40.0	41.0	38.0	41.0
22	39.88709856639763	41.0	40.0	41.0	38.0	41.0
23	39.823238097307566	41.0	40.0	41.0	38.0	41.0
24	39.79772225676441	41.0	40.0	41.0	38.0	41.0
25	39.77239506760584	41.0	40.0	41.0	37.0	41.0
26	39.66833851960023	41.0	40.0	41.0	37.0	41.0
27	39.60340589826949	41.0	40.0	41.0	37.0	41.0
28	39.52788311910866	41.0	40.0	41.0	36.0	41.0
29	39.44504491335123	41.0	39.0	41.0	36.0	41.0
30	39.34798920835064	41.0	39.0	41.0	35.0	41.0
31	39.24838177114019	41.0	39.0	41.0	35.0	41.0
32	39.15072751776755	41.0	39.0	41.0	35.0	41.0
33	39.04750903625317	41.0	39.0	41.0	35.0	41.0
34	38.97106226038928	41.0	39.0	41.0	35.0	41.0
35	38.864264834937444	41.0	39.0	41.0	35.0	41.0
36	38.77589598296014	41.0	38.0	41.0	35.0	41.0
37	38.67970797555829	41.0	38.0	41.0	35.0	41.0
38	38.574083448112724	40.0	38.0	41.0	35.0	41.0
39	38.44906881775338	40.0	38.0	41.0	35.0	41.0
40	38.31457470224441	40.0	38.0	41.0	35.0	41.0
41	38.17439437720103	40.0	38.0	41.0	35.0	41.0
42	38.00075114857581	40.0	37.0	41.0	34.0	41.0
43	37.20105447234652	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	11.0
12	8.0
13	9.0
14	10.0
15	8.0
16	4.0
17	15.0
18	38.0
19	56.0
20	102.0
21	225.0
22	392.0
23	606.0
24	1142.0
25	1713.0
26	2654.0
27	4060.0
28	5893.0
29	8597.0
30	11680.0
31	14486.0
32	17896.0
33	23543.0
34	34686.0
35	51327.0
36	83392.0
37	184755.0
38	363965.0
39	1213616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.917696635733435	17.223920798024988	13.613617271775036	25.244765294466536
2	19.08742115038824	20.161153716802666	34.25558509337996	26.495840039429126
3	19.76868969760961	22.071471219058335	29.143083185877416	29.016755897454637
4	14.308071661845851	16.142385373295163	32.88480067400892	36.66474229085006
5	15.782762498277691	35.458262362715374	31.79101772483467	16.967957414172265
6	33.556290955746434	33.721632535746224	16.67767133076761	16.04440517773973
7	30.123971615374394	30.103328610464033	20.052061855924666	19.72063791823691
8	29.366007884837714	31.56182110811453	19.02978864624853	20.04238236079923
9	27.96811100207961	14.355876515322494	18.88311466399065	38.792897818607244
10	19.104755348291448	25.740098641956955	29.292325197454293	25.862820812297304
11	36.29978581647776	22.021048951083486	19.808444766874793	21.870720465563963
12	23.662958004325155	26.03132304376663	26.810324860647373	23.49539409126085
13	31.460630875910354	18.72819335680446	24.473566335901197	25.337609431383985
14	24.009691347568445	19.912400569114805	24.614906718804246	31.4630013645125
15	26.845536493425104	26.092214969734297	21.745282110366986	25.31696642647362
16	25.46759122306841	25.04519978527324	23.785927100561562	25.701281891096787
17	23.911414840937745	26.28289114666954	24.56562030995126	25.240073702441457
18	24.669971193624963	23.68458871281975	26.45099829670517	25.194441796850114
19	25.06371922747752	24.91887249685046	26.305558943927576	23.711849331744446
20	25.734913198139758	23.490356802981285	26.305015706956247	24.46971429192271
21	27.314794466291897	23.49045557333971	25.462158853355156	23.73259110701324
22	26.735358158604456	23.45805889577702	24.901439528588835	24.905143417029688
23	25.02623587645606	24.02243272380499	25.554805449555758	25.396525950183197
24	24.4259590231414	24.63357431654616	26.07937482313933	24.861091837173113
25	24.62656162109814	23.793878114414596	25.89299515679547	25.686565107691788
26	25.028655750237423	24.398747789395916	25.978579672368845	24.594016787997823
27	25.12303082771042	23.970825211529068	25.455689394878462	25.45045456588205
28	24.17641571258616	24.186737215041344	26.714270687081182	24.922576385291315
29	24.023519197747643	24.49751814781873	26.910576774446525	24.5683858799871
30	23.687601208751648	24.81106465063196	26.98509900987654	24.516235130739854
31	24.473023098929872	24.497715688535575	25.929984656024818	25.099276556509732
32	23.726615500328656	24.112412520328174	26.880649355844415	25.28032262349875
33	23.555248928465076	23.680588513303626	27.560584503227076	25.20357805500423
34	24.457713693374338	23.44146547556199	26.783558093514788	25.317262737548884
35	23.85654790683387	23.71130609477312	27.337067182116243	25.09507881627676
36	22.726911317552133	23.847905500471875	27.499198725467295	25.925984456508694
37	23.148907673913612	23.037395939254253	28.021545765986357	25.79215062084578
38	22.459441186943153	22.4678860525883	29.190937424533274	25.881735335935275
39	22.76098709120801	22.394055209667247	29.3486243017553	25.496333397369447
40	22.207576772964973	22.83936137061651	29.43978934257955	25.513272513838963
41	21.116164312392865	23.300569559271842	30.227779262076776	25.355486866258513
42	21.363830986138073	23.433218150633685	29.77536163532107	25.42758922790717
43	20.384374726838228	23.350695516171424	29.95497553211296	26.30995422487739
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	175.0
1	246.5
2	318.0
3	1231.5
4	2145.0
5	2145.0
6	2830.5
7	3516.0
8	3374.0
9	3232.0
10	5089.5
11	6947.0
12	6947.0
13	11115.0
14	15283.0
15	23144.0
16	31005.0
17	27302.0
18	23599.0
19	23599.0
20	25078.5
21	26558.0
22	20613.5
23	14669.0
24	15109.5
25	15550.0
26	15550.0
27	17435.5
28	19321.0
29	23204.0
30	27087.0
31	31521.0
32	35955.0
33	35955.0
34	42642.5
35	49330.0
36	57658.0
37	65986.0
38	75036.0
39	84086.0
40	84086.0
41	92272.0
42	100458.0
43	109722.0
44	118986.0
45	129112.0
46	139238.0
47	139238.0
48	148965.5
49	158693.0
50	157418.5
51	156144.0
52	151555.0
53	146966.0
54	146966.0
55	141833.0
56	136700.0
57	131326.5
58	125953.0
59	122060.0
60	118167.0
61	118167.0
62	109908.5
63	101650.0
64	93386.0
65	85122.0
66	76543.0
67	67964.0
68	67964.0
69	58828.0
70	49692.0
71	42621.0
72	35550.0
73	28291.5
74	21033.0
75	21033.0
76	17135.5
77	13238.0
78	10891.5
79	8545.0
80	7511.0
81	6477.0
82	6477.0
83	5197.0
84	3917.0
85	3338.0
86	2759.0
87	2303.0
88	1847.0
89	1847.0
90	1338.5
91	830.0
92	485.5
93	141.0
94	90.5
95	40.0
96	40.0
97	31.5
98	23.0
99	13.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2024899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.811465154398135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25055845469272	49.86271154650848
2	7.815956463558179	9.037037894825731
3	2.23376793689833	3.874121917410288
4	1.025322316233649	2.371015414278735
5	0.5955501120130692	1.7214812274170728
6	0.3793064080805039	1.3156955516151583
7	0.2574522775730484	1.0418585361684307
8	0.19332549520661518	0.8941144103675198
9	0.1559813657937409	0.8115760163988253
>10	0.9115940471462183	10.152045741337853
>50	0.09797284493873248	3.939340825614672
>100	0.07342408069782128	8.408654339388269
>500	0.0077275240795250475	3.0899977703392136
>1k	0.0018030889518891777	1.5468602997073284
>5k	0.0	0.0
>10k+	2.5758413598416825E-4	1.9334885086224003
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16395	0.8096700131710273	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11398	0.5628922726516236	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11159	0.5510892148200972	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4920	0.2429750817201253	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2331	0.11511685274179106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.9385179211407584E-5	0.0
2	0.0	0.0	0.0	9.877035842281517E-5	0.0
3	0.0	0.0	0.0	1.4815553763422275E-4	0.0
4	0.0	0.0	0.0	2.469258960570379E-4	0.0
5	0.0	4.9385179211407584E-5	0.0	2.469258960570379E-4	0.0
6	0.0	4.9385179211407584E-5	0.0	2.469258960570379E-4	0.0
7	0.0	4.9385179211407584E-5	0.0	3.456962544798531E-4	0.0
8	0.0	4.9385179211407584E-5	0.0	3.456962544798531E-4	0.0
9	0.0	4.9385179211407584E-5	0.0	6.913925089597062E-4	0.0
10	0.0	4.9385179211407584E-5	0.0	0.001284014659496597	4.9385179211407584E-5
11	0.0	4.9385179211407584E-5	0.0	0.002370488602147564	4.9385179211407584E-5
12	0.0	4.9385179211407584E-5	0.0	0.0033581921863757155	4.9385179211407584E-5
13	0.0	4.9385179211407584E-5	0.0	0.003901429157701199	9.877035842281517E-5
14	0.0	4.9385179211407584E-5	0.0	0.005037288279563574	9.877035842281517E-5
15	0.0	4.9385179211407584E-5	0.0	0.006765769551962839	9.877035842281517E-5
16	0.0	4.9385179211407584E-5	0.0	0.009531339587801663	9.877035842281517E-5
17	0.0	4.9385179211407584E-5	0.0	0.012543835519697526	9.877035842281517E-5
18	0.0	4.9385179211407584E-5	0.0	0.013580924283137084	9.877035842281517E-5
19	0.0	4.9385179211407584E-5	0.0	0.014864938942633682	9.877035842281517E-5
20	0.0	4.9385179211407584E-5	0.0	0.01654403503582154	1.4815553763422275E-4
21	0.0	4.9385179211407584E-5	0.0	0.01960591614692881	1.4815553763422275E-4
22	0.0	4.9385179211407584E-5	0.0	0.02740877446233121	1.4815553763422275E-4
23	0.0	4.9385179211407584E-5	0.0	0.03822412870962947	1.4815553763422275E-4
24	0.0	4.9385179211407584E-5	0.0	0.05585463768810198	1.4815553763422275E-4
25	0.0	4.9385179211407584E-5	0.0	0.06365749600350437	1.4815553763422275E-4
26	0.0	4.9385179211407584E-5	0.0	0.07570747973108782	1.4815553763422275E-4
27	0.0	4.9385179211407584E-5	0.0	0.13783403517903856	1.4815553763422275E-4
28	0.0	4.9385179211407584E-5	0.0	0.4000199516124014	1.4815553763422275E-4
29	0.0	4.9385179211407584E-5	0.0	0.797027407292907	1.4815553763422275E-4
30	0.0	4.9385179211407584E-5	0.0	1.4185892728476828	1.4815553763422275E-4
31	0.0	4.9385179211407584E-5	0.0	2.516718117792542	1.4815553763422275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACACA	1185	0.0	18.734177	37
AATCGTA	50	0.0070366883	18.5	18
AGTCGGT	1545	0.0	17.482203	11
GGTATCA	6910	0.0	17.321997	1
GCAGTCG	1600	0.0	16.996876	9
GGCAGTC	1640	0.0	16.582315	8
TCGGTGA	1660	0.0	16.38253	13
CAGTCGG	1655	0.0	16.320242	10
CGGTGAT	1645	0.0	16.082067	14
GGGCAGT	1735	0.0	15.887609	7
TACCTTA	70	0.0025934083	15.857142	31
GATACAC	200	3.6379788E-12	15.725	3
GTATAGA	165	9.749783E-10	15.696969	1
CGCCTTA	1250	0.0	15.688	25
ATACACT	295	0.0	15.677966	4
CTTATAC	5485	0.0	15.616227	37
GTCGGTG	1755	0.0	15.179487	12
ATTAGAC	100	1.0940172E-4	14.8	3
CGCTTAT	425	0.0	14.8	26
TTATACA	3280	0.0	14.777438	37
>>END_MODULE