FastQCFastQC Report
Fri 10 Feb 2017
ERR1631454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904930
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.30433293182898125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23260.2570364558584642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20730.22907849225906976No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11620.12840772214425425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.11238438332246695No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10150.11216337175251123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.020.7769221
CCGCTTA909.472751E-820.55555725
TCTAGGG555.1419326E-420.1818183
AGTCGGT3550.019.80281611
AAGACGG2900.018.55
TATACAC4700.018.49999837
GCAGTCG3900.018.0256429
ACGGACC2800.017.8392858
CGCTTAT953.604673E-617.52631626
CAAGACG3300.016.8181824
CAGTCGG4400.016.81818210
CTTATAC12850.016.55642137
CTAGATA2250.016.4444453
CTTATTG4200.016.2976228
AATGCCG803.3815714E-416.18755
GATTCCT4350.016.16092118
GACGGAC3100.016.1129047
TAGATAA2300.016.0869564
ATACCAT1151.2414039E-616.0869566
AGACGGA3350.016.0149256
Produced by FastQC (version 0.11.5)