FastQCFastQC Report
Fri 10 Feb 2017
ERR1631453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89660
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC2760.3078295784073165No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT1680.18737452598706225No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT1120.12491635065804149No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT1040.1159937541824671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATGA200.001832899937.05
GACAGGC706.4210326E-926.428577
GGTATCA508.969637E-625.9000021
CAGGACA659.1913535E-825.6153854
CATCAGA753.6618258E-722.216
ACAGGCT806.8140616E-720.81258
TCAGAAG806.8140616E-720.812518
GCTTTAT450.003798769820.5555551
GTATCAA656.811858E-519.9230772
ATCAGAA851.2193213E-619.58823617
GCTGCAT851.2193213E-619.58823612
TGCATCA851.2193213E-619.58823614
GGACAGG851.2193213E-619.5882366
AGAAGAG801.5897558E-518.520
ATTCCAT701.20336445E-418.56
TCCAGGA1002.802226E-718.52
TTCCATC500.00698563918.57
TATTCCA609.146581E-418.55
TTTATTC500.00698563918.53
CTCTAAG500.00698563918.52
Produced by FastQC (version 0.11.5)