##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631453.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89660
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07806156591568	34.0	33.0	34.0	31.0	34.0
2	33.219384340843185	34.0	33.0	34.0	31.0	34.0
3	33.300635734998885	34.0	34.0	34.0	31.0	34.0
4	36.53098371626143	37.0	37.0	37.0	35.0	37.0
5	36.51845862145885	37.0	37.0	37.0	35.0	37.0
6	36.551985277715815	37.0	37.0	37.0	35.0	37.0
7	36.562826232433636	37.0	37.0	37.0	35.0	37.0
8	36.542705777381215	37.0	37.0	37.0	35.0	37.0
9	38.41053981708677	39.0	39.0	39.0	37.0	39.0
10	38.36919473566808	39.0	39.0	39.0	37.0	39.0
11	38.43708454160161	39.0	39.0	39.0	37.0	39.0
12	38.38680571046174	39.0	39.0	39.0	37.0	39.0
13	38.408153022529554	39.0	39.0	39.0	37.0	39.0
14	39.966618335935756	41.0	40.0	41.0	38.0	41.0
15	39.968815525317865	41.0	40.0	41.0	38.0	41.0
16	39.949788088333705	41.0	40.0	41.0	38.0	41.0
17	39.93161945126032	41.0	40.0	41.0	38.0	41.0
18	39.84551639527103	41.0	40.0	41.0	38.0	41.0
19	39.87072273031452	41.0	40.0	41.0	38.0	41.0
20	39.84433415123801	41.0	40.0	41.0	38.0	41.0
21	39.812826232433636	41.0	40.0	41.0	38.0	41.0
22	39.758889136738794	41.0	40.0	41.0	38.0	41.0
23	39.70123801026099	41.0	40.0	41.0	37.0	41.0
24	39.667744813740796	41.0	40.0	41.0	37.0	41.0
25	39.63604728976132	41.0	40.0	41.0	37.0	41.0
26	39.530615659156815	41.0	40.0	41.0	37.0	41.0
27	39.48833370510819	41.0	40.0	41.0	37.0	41.0
28	39.41046174436761	41.0	39.0	41.0	36.0	41.0
29	39.35913450814187	41.0	39.0	41.0	36.0	41.0
30	39.30364711130939	41.0	39.0	41.0	36.0	41.0
31	39.27472674548294	41.0	39.0	41.0	35.0	41.0
32	39.20188489850546	41.0	39.0	41.0	35.0	41.0
33	39.150680347981265	41.0	39.0	41.0	35.0	41.0
34	39.083604728976134	41.0	39.0	41.0	35.0	41.0
35	38.98344858353781	41.0	39.0	41.0	35.0	41.0
36	38.94290653580192	41.0	39.0	41.0	35.0	41.0
37	38.88272362257417	40.0	38.0	41.0	35.0	41.0
38	38.829221503457504	40.0	38.0	41.0	35.0	41.0
39	38.74706669640865	40.0	38.0	41.0	35.0	41.0
40	38.64537140307829	40.0	38.0	41.0	35.0	41.0
41	38.582020968101716	40.0	38.0	41.0	35.0	41.0
42	38.481006022752624	40.0	37.0	41.0	35.0	41.0
43	37.64522641088557	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	3.0
20	4.0
21	14.0
22	23.0
23	22.0
24	61.0
25	75.0
26	110.0
27	155.0
28	194.0
29	315.0
30	396.0
31	497.0
32	694.0
33	1039.0
34	1632.0
35	2650.0
36	4039.0
37	7013.0
38	17407.0
39	53314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60852107963417	15.49855007807272	11.152130269908543	28.740798572384563
2	21.768904751282623	17.318759759089893	31.29154583983939	29.620789649788087
3	22.03546732099041	18.796564800356904	26.473343743029222	32.69462413562347
4	17.691278161945124	13.90586660718269	28.985054650903415	39.41780057996877
5	19.15012268570154	32.05888913673879	29.957617666741022	18.833370510818646
6	39.862815079188046	29.240463975016727	13.99620789649788	16.900513049297345
7	33.95271023867946	25.97144769127816	17.95784073165291	22.11800133838947
8	30.30894490296676	29.630827570823108	17.687932188266785	22.372295337943342
9	28.485389248271247	13.286861476689719	16.6897167075619	41.53803256747713
10	21.012714699977693	23.02810617889806	28.20990408208789	27.74927503903636
11	39.91858130716038	18.86459959848316	17.963417354450144	23.253401739906312
12	26.113093910327905	21.506803479812625	24.452375641311622	27.92772696854785
13	33.321436538032565	15.975908989515949	21.628373856792326	29.07428061565916
14	25.305598929288426	17.590898951594912	20.827570823109525	36.27593129600714
15	29.627481597144765	22.8217711354004	18.496542493865714	29.054204773589117
16	29.1110863261209	21.685255409324114	19.42783850100379	29.77581976355119
17	28.196520187374524	22.534017399063128	20.93352665625697	28.335935757305375
18	27.845192951148785	20.364711130939103	22.10127146999777	29.688824447914342
19	28.72406870399286	21.6729868391702	20.98594691055097	28.616997546285972
20	28.593575730537584	20.945795226410883	21.248048182020966	29.212580861030563
21	31.148784296230204	20.852107963417353	20.38590229756859	27.61320544278385
22	30.896720945795224	21.008253401739907	19.568369395494088	28.526656256970778
23	29.407762658933752	21.311621681909436	20.20633504349766	29.07428061565916
24	29.31965201873745	21.419808164175773	20.559892928842295	28.70064688824448
25	28.680571046174435	20.854338612536246	21.02052197189382	29.444568369395498
26	29.406647334374302	21.56480035690386	21.554762435868838	27.473789872853
27	29.80481820209681	20.684809279500335	20.660272139192504	28.850100379210353
28	28.13517733660495	20.36248048182021	22.172652241802364	29.329689939772475
29	27.633281284853894	20.771804595137187	22.719161275931295	28.875752844077628
30	27.24403301360696	21.329466874860582	22.51282623243364	28.913673879098816
31	28.08052643319206	21.09747936649565	21.511264778050414	29.31072942226188
32	26.991969663171982	20.56770020075842	22.967878652687933	29.47245148338166
33	28.06379656480036	20.433861253624805	22.213919250501895	29.288422931072944
34	28.06937318759759	20.176221280392596	23.175329020745036	28.579076511264777
35	27.78719607405755	19.35534240463975	24.32411331697524	28.533348204327456
36	26.40084764666518	19.51594914120009	24.12447022083426	29.95873299130047
37	25.538701762212806	18.347089002899843	25.743921481151016	30.370287753736335
38	25.409324113316977	17.50836493419585	27.796118670533126	29.286192281954047
39	24.502565246486725	17.527325451706446	28.731875975908988	29.238233325897838
40	23.827793888021414	17.664510372518404	29.782511710907873	28.72518402855231
41	22.27526210127147	17.92326567031006	30.71380771804595	29.08766451037252
42	21.526879321882667	19.008476466651796	30.523087218380553	28.941556993084987
43	20.343519964309614	18.40062458175329	31.278161945126033	29.977693508811065
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	6.0
4	8.0
5	8.0
6	9.0
7	10.0
8	7.5
9	5.0
10	8.5
11	12.0
12	12.0
13	28.5
14	45.0
15	47.5
16	50.0
17	60.0
18	70.0
19	70.0
20	75.5
21	81.0
22	82.5
23	84.0
24	120.0
25	156.0
26	156.0
27	194.0
28	232.0
29	327.0
30	422.0
31	538.0
32	654.0
33	654.0
34	847.5
35	1041.0
36	1359.5
37	1678.0
38	2004.5
39	2331.0
40	2331.0
41	2606.5
42	2882.0
43	3269.0
44	3656.0
45	3988.5
46	4321.0
47	4321.0
48	4755.0
49	5189.0
50	5894.5
51	6600.0
52	7337.0
53	8074.0
54	8074.0
55	8181.5
56	8289.0
57	8025.5
58	7762.0
59	7478.5
60	7195.0
61	7195.0
62	7014.5
63	6834.0
64	6220.0
65	5606.0
66	4965.5
67	4325.0
68	4325.0
69	3748.5
70	3172.0
71	2869.5
72	2567.0
73	2416.5
74	2266.0
75	2266.0
76	2091.5
77	1917.0
78	1546.5
79	1176.0
80	827.5
81	479.0
82	479.0
83	356.0
84	233.0
85	170.5
86	108.0
87	91.5
88	75.0
89	75.0
90	55.5
91	36.0
92	22.5
93	9.0
94	5.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	89660.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.77782734775819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.23867238485921	64.77135846531341
2	4.872894524209087	6.995315636850323
3	1.6268879358567967	3.5032344412223955
4	0.8359748896761763	2.4001784519295115
5	0.5438498352911927	1.9518179790318984
6	0.3185406178134129	1.3718492081195628
7	0.2641556342842936	1.3272362257416908
8	0.1600472372428367	0.9190274369841624
9	0.12430853378084405	0.8030336828016953
>10	0.9696065634905836	13.215480704885122
>50	0.03884641680651377	2.0053535578853445
>100	0.006215426689042202	0.7361142092348874
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	276	0.3078295784073165	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	168	0.18737452598706225	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	112	0.12491635065804149	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	104	0.1159937541824671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.001115324559446799	0.0	0.0	0.0	0.0
22	0.001115324559446799	0.0	0.0	0.0	0.0
23	0.001115324559446799	0.0	0.0	0.0033459736783403972	0.0
24	0.001115324559446799	0.0	0.0	0.012268570153914788	0.0
25	0.001115324559446799	0.0	0.0	0.02230649118893598	0.0
26	0.001115324559446799	0.0	0.0	0.04238233325897836	0.0
27	0.001115324559446799	0.0	0.0	0.15949141200089226	0.0
28	0.001115324559446799	0.0	0.0	0.5743921481151015	0.0
29	0.001115324559446799	0.0	0.0	1.0606736560339058	0.0
30	0.001115324559446799	0.0	0.0	1.6339504795895605	0.0
31	0.001115324559446799	0.0	0.0	3.0214142315413786	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATGA	20	0.0018328999	37.0	5
GACAGGC	70	6.4210326E-9	26.42857	7
GGTATCA	50	8.969637E-6	25.900002	1
CAGGACA	65	9.1913535E-8	25.615385	4
CATCAGA	75	3.6618258E-7	22.2	16
ACAGGCT	80	6.8140616E-7	20.8125	8
TCAGAAG	80	6.8140616E-7	20.8125	18
GCTTTAT	45	0.0037987698	20.555555	1
GTATCAA	65	6.811858E-5	19.923077	2
ATCAGAA	85	1.2193213E-6	19.588236	17
GCTGCAT	85	1.2193213E-6	19.588236	12
TGCATCA	85	1.2193213E-6	19.588236	14
GGACAGG	85	1.2193213E-6	19.588236	6
AGAAGAG	80	1.5897558E-5	18.5	20
ATTCCAT	70	1.20336445E-4	18.5	6
TCCAGGA	100	2.802226E-7	18.5	2
TTCCATC	50	0.006985639	18.5	7
TATTCCA	60	9.146581E-4	18.5	5
TTTATTC	50	0.006985639	18.5	3
CTCTAAG	50	0.006985639	18.5	2
>>END_MODULE