FastQCFastQC Report
Fri 10 Feb 2017
ERR1631452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129096
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3450.2672429819669084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.2656937472888393No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3030.23470905372745865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2360.182809692012146No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1600.1239387742455227No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1440.11154489682097045No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1430.11077027948193593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGCT200.001835888337.012
CGGCTTA250.005478505529.59999814
TATAGGG250.005478505529.5999982
CTTCGCG400.001921961823.1250022
GCGGCTT400.001921961823.12500213
GGTATCA3350.022.0895521
GGCTTAA450.003807707520.55555515
TTGCAGT609.1763865E-418.54
TATACAC609.1763865E-418.537
TTATACA1054.7162575E-717.61904737
GCTGTGA752.0494076E-417.2666668
CTTATAC2100.016.73809437
GGGCAGT2950.016.3050847
CTCTATG3600.015.9305551
GGCAGTC3050.015.7704918
GTATCAA4700.015.7446792
TCTATGG3800.015.5789482
GCAGTCG3000.015.4166679
TGGGCAG3350.014.9104486
CAGTCGG3000.014.810
Produced by FastQC (version 0.11.5)