##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631452.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129096
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03131003284378	34.0	33.0	34.0	31.0	34.0
2	33.1837314866456	34.0	33.0	34.0	31.0	34.0
3	33.25181260457334	34.0	34.0	34.0	31.0	34.0
4	36.47357005639214	37.0	37.0	37.0	35.0	37.0
5	36.46514996591684	37.0	37.0	37.0	35.0	37.0
6	36.530891739480694	37.0	37.0	37.0	35.0	37.0
7	36.5377083720642	37.0	37.0	37.0	35.0	37.0
8	36.53455567949433	37.0	37.0	37.0	35.0	37.0
9	38.38771147053355	39.0	39.0	39.0	37.0	39.0
10	38.348097539815335	39.0	39.0	39.0	37.0	39.0
11	38.41518714754911	39.0	39.0	39.0	37.0	39.0
12	38.36754818119849	39.0	39.0	39.0	37.0	39.0
13	38.38561225754477	39.0	39.0	39.0	37.0	39.0
14	39.92669021503377	41.0	40.0	41.0	38.0	41.0
15	39.910980975398154	41.0	40.0	41.0	38.0	41.0
16	39.863969449092146	41.0	40.0	41.0	38.0	41.0
17	39.821969696969695	41.0	40.0	41.0	38.0	41.0
18	39.844007560265226	41.0	40.0	41.0	38.0	41.0
19	39.85883373613435	41.0	40.0	41.0	38.0	41.0
20	39.84778769287972	41.0	40.0	41.0	38.0	41.0
21	39.82318584619198	41.0	40.0	41.0	38.0	41.0
22	39.76251007002541	41.0	40.0	41.0	38.0	41.0
23	39.72042510999566	41.0	40.0	41.0	37.0	41.0
24	39.682770961145195	41.0	40.0	41.0	37.0	41.0
25	39.66579134907356	41.0	40.0	41.0	37.0	41.0
26	39.58407696597881	41.0	40.0	41.0	37.0	41.0
27	39.52515182499845	41.0	40.0	41.0	37.0	41.0
28	39.45322860506909	41.0	39.0	41.0	36.0	41.0
29	39.40231300737436	41.0	39.0	41.0	36.0	41.0
30	39.3273455413026	41.0	39.0	41.0	36.0	41.0
31	39.25043378570986	41.0	39.0	41.0	35.0	41.0
32	39.16684482865465	41.0	39.0	41.0	35.0	41.0
33	39.085602962136704	41.0	39.0	41.0	35.0	41.0
34	39.0444475429138	41.0	39.0	41.0	35.0	41.0
35	38.94507188448906	41.0	39.0	41.0	35.0	41.0
36	38.8855890190246	41.0	39.0	41.0	35.0	41.0
37	38.81756212431059	41.0	39.0	41.0	35.0	41.0
38	38.73216830885543	40.0	38.0	41.0	35.0	41.0
39	38.64639493090414	40.0	38.0	41.0	35.0	41.0
40	38.566067112846255	40.0	38.0	41.0	35.0	41.0
41	38.47083565718535	40.0	38.0	41.0	35.0	41.0
42	38.36059211749396	40.0	38.0	41.0	35.0	41.0
43	37.546833364318026	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	1.0
18	1.0
19	1.0
20	10.0
21	24.0
22	30.0
23	58.0
24	77.0
25	123.0
26	177.0
27	272.0
28	398.0
29	505.0
30	640.0
31	846.0
32	1071.0
33	1500.0
34	2274.0
35	3450.0
36	5506.0
37	10837.0
38	24750.0
39	76542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71085083968519	17.09425543781372	12.922166449773812	27.27272727272727
2	20.868191113589887	19.041643428146497	33.08623040218132	27.003935056082295
3	20.995228357191547	20.42665923034021	28.086075478713518	30.492036933754722
4	15.380027266530332	15.727055834417797	33.11101815703042	35.78189874202144
5	16.62948503439301	34.751657681105534	31.970006816632583	16.648850467868872
6	35.056082295346094	31.865433475862925	16.400198302038792	16.678285926752185
7	31.54551651484167	28.790202639895888	19.054811922910083	20.60946892235236
8	30.027266530334014	29.795655945962697	18.73179649253269	21.4452810311706
9	28.839003532255063	13.471370143149283	18.449061163785093	39.24056516081056
10	19.606339468302657	24.407417735638596	29.585734647084337	26.400508148974406
11	36.70059490611638	21.659850034083163	18.45913118919254	23.180423870607918
12	24.284253578732105	25.069715560513107	25.691733283757824	24.954297576996964
13	32.870112164590694	18.16942430439363	23.120778335502262	25.839685195513418
14	24.876835843093513	20.00294354588833	22.724174257916587	32.39604635310157
15	30.083038978744504	24.253268885170726	19.89837020511867	25.765321930966103
16	27.52292867323542	23.982152816508645	22.252432298444567	26.242486211811368
17	24.683181508334883	25.101474871413522	23.53519861188573	26.68014500836587
18	25.878416062465142	22.261727706512982	25.06816632583504	26.79168990518684
19	25.997707132676457	24.787754849104545	24.439951663878045	24.774586354340954
20	27.406736072380244	22.252432298444567	24.931059056825926	25.409772572349258
21	28.812666542727893	22.41200347028568	23.23697093635744	25.53835905062899
22	28.5097911631654	22.185040589948564	22.6637541054719	26.641414141414145
23	26.621274090599243	22.824099894652043	23.13936915163909	27.415256863109622
24	26.044184173018532	23.140918386317157	24.178131003284378	26.636766437379933
25	26.05425419842598	22.703259589762656	23.370205118671375	27.87228109313999
26	26.88309475119291	22.88529466443577	23.352388919873583	26.879221664497738
27	26.749085951539943	22.203631406085396	23.223027824254817	27.824254818119847
28	25.638284687364443	22.51890066307244	24.264113527917207	27.578701121645903
29	24.62431059056826	23.260209456528475	25.543006754663196	26.572473198240072
30	24.916341327384274	24.182778707318583	25.4601226993865	25.44075726591064
31	25.95200470967342	24.187426411352792	23.23697093635744	26.623597942616346
32	24.518188015120533	23.748992997459258	25.37878787878788	26.354031108632338
33	25.256398339220425	22.531294540496994	25.74208341079507	26.470223709487513
34	26.079816570614117	22.304331660159882	24.62121212121212	26.99463964801388
35	25.2176674722687	23.148664559707505	25.03253392823945	26.60113403978435
36	23.924056516081055	23.596393381669458	25.604976141785958	26.874573960463533
37	25.00309846935614	22.308204746855054	25.801728945900727	26.886967837888083
38	23.972082791101197	21.174939579847553	27.687922166449773	27.165055462601472
39	24.27805664001983	20.820939455908782	28.314587593728696	26.586416310342692
40	23.271054099274956	20.372436016607796	28.582605193034645	27.773904691082606
41	21.834138935365928	20.808545578484228	29.81037367540435	27.54694181074549
42	21.872095184978622	20.792278614364506	29.781712833860073	27.5539133667968
43	20.693127594968086	21.105998636673483	30.499783107145067	27.701090661213364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	4.0
2	7.0
3	16.0
4	25.0
5	25.0
6	41.5
7	58.0
8	51.5
9	45.0
10	81.0
11	117.0
12	117.0
13	201.5
14	286.0
15	468.5
16	651.0
17	597.0
18	543.0
19	543.0
20	676.5
21	810.0
22	672.5
23	535.0
24	592.5
25	650.0
26	650.0
27	780.5
28	911.0
29	1160.0
30	1409.0
31	1729.0
32	2049.0
33	2049.0
34	2388.0
35	2727.0
36	3200.5
37	3674.0
38	4218.5
39	4763.0
40	4763.0
41	5193.0
42	5623.0
43	6306.5
44	6990.0
45	7457.0
46	7924.0
47	7924.0
48	9331.5
49	10739.0
50	11082.0
51	11425.0
52	11098.0
53	10771.0
54	10771.0
55	10280.0
56	9789.0
57	9554.5
58	9320.0
59	8881.5
60	8443.0
61	8443.0
62	7883.0
63	7323.0
64	6674.0
65	6025.0
66	5403.5
67	4782.0
68	4782.0
69	4152.5
70	3523.0
71	3062.5
72	2602.0
73	2082.0
74	1562.0
75	1562.0
76	1249.5
77	937.0
78	812.5
79	688.0
80	622.5
81	557.0
82	557.0
83	458.0
84	359.0
85	274.0
86	189.0
87	173.0
88	157.0
89	157.0
90	109.5
91	62.0
92	44.0
93	26.0
94	19.5
95	13.0
96	13.0
97	9.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	129096.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.32385201710356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.98473282442748	71.12613868748838
2	4.252569573841438	6.576501208403049
3	1.2892949450021036	2.99079754601227
4	0.7132696199234637	2.2061101815703044
5	0.4027168359680231	1.556980851459379
6	0.27348680651559776	1.2688232013385388
7	0.20636733385426057	1.1169982028877734
8	0.12221754723407666	0.7560265228976886
9	0.12522289675622608	0.8714445064138315
>10	0.5900502895153372	8.174536778831257
>50	0.02404279617719541	1.2324161864039165
>100	0.01602853078479694	2.123226126293611
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.2672429819669084	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.2656937472888393	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	303	0.23470905372745865	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	236	0.182809692012146	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	160	0.1239387742455227	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	144	0.11154489682097045	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	143	0.11077027948193593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	7.74617339034517E-4	0.0	0.0	0.0
4	0.0	7.74617339034517E-4	0.0	0.0	0.0
5	0.0	7.74617339034517E-4	0.0	0.0	0.0
6	0.0	7.74617339034517E-4	0.0	0.0	0.0
7	0.0	7.74617339034517E-4	0.0	0.0	0.0
8	0.0	7.74617339034517E-4	0.0	0.0	0.0
9	0.0	7.74617339034517E-4	0.0	0.0	0.0
10	0.0	7.74617339034517E-4	0.0	7.74617339034517E-4	0.0
11	0.0	7.74617339034517E-4	0.0	7.74617339034517E-4	0.0
12	0.0	7.74617339034517E-4	0.0	7.74617339034517E-4	0.0
13	0.0	7.74617339034517E-4	0.0	7.74617339034517E-4	0.0
14	0.0	7.74617339034517E-4	0.0	7.74617339034517E-4	0.0
15	0.0	7.74617339034517E-4	0.0	7.74617339034517E-4	0.0
16	0.0	7.74617339034517E-4	0.0	0.005422321373241619	0.0
17	0.0	7.74617339034517E-4	0.0	0.006971556051310652	0.0
18	0.0	7.74617339034517E-4	0.0	0.00774617339034517	0.0
19	0.0	7.74617339034517E-4	0.0	0.009295408068414203	0.0
20	0.0	7.74617339034517E-4	0.0	0.01007002540744872	0.0
21	0.0	7.74617339034517E-4	0.0	0.012393877424552271	0.0
22	0.0	7.74617339034517E-4	0.0	0.018590816136828406	0.0
23	0.0	7.74617339034517E-4	0.0	0.027111606866208095	0.0
24	0.0	7.74617339034517E-4	0.0	0.04182933630786392	0.0
25	0.0	7.74617339034517E-4	0.0	0.047251657681105536	0.0
26	0.0	7.74617339034517E-4	0.0	0.05809630042758877	0.0
27	0.0	7.74617339034517E-4	0.0	0.10844642746483238	0.0
28	0.0	7.74617339034517E-4	0.0	0.22541364565904443	0.0
29	0.0	7.74617339034517E-4	0.0	0.4159695110615356	0.0
30	0.0	7.74617339034517E-4	0.0	0.6731424676209953	0.0
31	0.0	7.74617339034517E-4	0.0	1.340862613868749	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGCT	20	0.0018358883	37.0	12
CGGCTTA	25	0.0054785055	29.599998	14
TATAGGG	25	0.0054785055	29.599998	2
CTTCGCG	40	0.0019219618	23.125002	2
GCGGCTT	40	0.0019219618	23.125002	13
GGTATCA	335	0.0	22.089552	1
GGCTTAA	45	0.0038077075	20.555555	15
TTGCAGT	60	9.1763865E-4	18.5	4
TATACAC	60	9.1763865E-4	18.5	37
TTATACA	105	4.7162575E-7	17.619047	37
GCTGTGA	75	2.0494076E-4	17.266666	8
CTTATAC	210	0.0	16.738094	37
GGGCAGT	295	0.0	16.305084	7
CTCTATG	360	0.0	15.930555	1
GGCAGTC	305	0.0	15.770491	8
GTATCAA	470	0.0	15.744679	2
TCTATGG	380	0.0	15.578948	2
GCAGTCG	300	0.0	15.416667	9
TGGGCAG	335	0.0	14.910448	6
CAGTCGG	300	0.0	14.8	10
>>END_MODULE