FastQCFastQC Report
Fri 10 Feb 2017
ERR1631451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739484
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22810.30845833040336235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.2599109649431225No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT17610.23813902667265283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16410.2219114950424891No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13780.18634615488638023No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG10940.14794099669499272No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG10040.13577034797236992No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG9910.1340123653791022No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT9580.12954979418080714No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT9260.12522245241276347No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9110.12319401095899302No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT9030.12211217551698211No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG7990.1080483147708402No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG7460.1008811549675179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA250.005495092429.59999837
TGCCGGA250.005495092429.5999987
TATAGAG502.70084E-422.1999992
GGTATCA8700.021.9022981
TAGATAA2100.020.2619064
TAGAGCA555.140709E-420.1818184
TGCATCA6050.019.87603214
CGGTGAT1600.019.6562514
GCATCAG6200.019.39516315
CTAGATA2200.019.3409083
ATACACA2800.019.16071337
ATCAGAA6450.018.93023317
TCTAGAT2250.018.9111122
GCTGCAT6400.018.78906212
GATAACC2300.018.56
AGATAAC2300.018.55
CTGCATC6400.018.513
GTATACT609.2325703E-418.54
AGTCGGT1501.2732926E-1118.511
TGTGCGT500.00703256918.49999810
Produced by FastQC (version 0.11.5)