##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631451.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739484
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.057688874945235	34.0	33.0	34.0	31.0	34.0
2	33.202569629633636	34.0	33.0	34.0	31.0	34.0
3	33.27518648138432	34.0	34.0	34.0	31.0	34.0
4	36.5021542048239	37.0	37.0	37.0	35.0	37.0
5	36.492739531889804	37.0	37.0	37.0	35.0	37.0
6	36.53698660146805	37.0	37.0	37.0	35.0	37.0
7	36.54710852432237	37.0	37.0	37.0	35.0	37.0
8	36.543767005100854	37.0	37.0	37.0	35.0	37.0
9	38.394680615131634	39.0	39.0	39.0	37.0	39.0
10	38.36236483818446	39.0	39.0	39.0	37.0	39.0
11	38.41217659881755	39.0	39.0	39.0	37.0	39.0
12	38.378447674324256	39.0	39.0	39.0	37.0	39.0
13	38.39173126125785	39.0	39.0	39.0	37.0	39.0
14	39.94871423857717	41.0	40.0	41.0	38.0	41.0
15	39.938085205359414	41.0	40.0	41.0	38.0	41.0
16	39.90603853497844	41.0	40.0	41.0	38.0	41.0
17	39.89426140389785	41.0	40.0	41.0	38.0	41.0
18	39.813607867107336	41.0	40.0	41.0	38.0	41.0
19	39.83213429905177	41.0	40.0	41.0	38.0	41.0
20	39.80278545580432	41.0	40.0	41.0	38.0	41.0
21	39.78414543113847	41.0	40.0	41.0	38.0	41.0
22	39.72088239907828	41.0	40.0	41.0	37.0	41.0
23	39.66094465870796	41.0	40.0	41.0	37.0	41.0
24	39.62680598904101	41.0	40.0	41.0	37.0	41.0
25	39.62458416950198	41.0	40.0	41.0	37.0	41.0
26	39.51122269041656	41.0	40.0	41.0	37.0	41.0
27	39.46887153745044	41.0	40.0	41.0	36.0	41.0
28	39.39118763894824	41.0	39.0	41.0	36.0	41.0
29	39.340426567714786	41.0	39.0	41.0	36.0	41.0
30	39.27766253225222	41.0	39.0	41.0	35.0	41.0
31	39.247126374607156	41.0	39.0	41.0	35.0	41.0
32	39.17466503670127	41.0	39.0	41.0	35.0	41.0
33	39.08718511827166	41.0	39.0	41.0	35.0	41.0
34	39.02449951587864	41.0	39.0	41.0	35.0	41.0
35	38.91168571598574	41.0	39.0	41.0	35.0	41.0
36	38.860363442616745	41.0	38.0	41.0	35.0	41.0
37	38.78566405764019	41.0	38.0	41.0	35.0	41.0
38	38.711876119023536	40.0	38.0	41.0	35.0	41.0
39	38.62437862076799	40.0	38.0	41.0	35.0	41.0
40	38.50899275711171	40.0	38.0	41.0	35.0	41.0
41	38.40526502263741	40.0	37.0	41.0	35.0	41.0
42	38.27864294562154	40.0	37.0	41.0	35.0	41.0
43	37.456684661196185	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	2.0
14	1.0
15	2.0
16	4.0
17	7.0
18	16.0
19	21.0
20	51.0
21	98.0
22	160.0
23	287.0
24	491.0
25	713.0
26	1024.0
27	1504.0
28	2101.0
29	2845.0
30	3827.0
31	5153.0
32	6641.0
33	8738.0
34	13859.0
35	22562.0
36	33103.0
37	60817.0
38	135440.0
39	440013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30805805128982	16.493257460607666	11.510864332426394	27.687820155676118
2	20.346349616759795	18.476126596383423	32.74229057018137	28.43523321667541
3	21.215469164985315	20.44696031286681	27.79316388184193	30.544406640305944
4	15.593846520005842	14.386923855012416	31.493852470100776	38.52537715488097
5	17.32694689810733	33.98193875729562	30.91601711463669	17.775097229960352
6	37.06273563728221	31.465048601457234	15.237246512432995	16.23496924882756
7	31.965803181678037	28.194660060258236	19.176479815655238	20.66305694240849
8	29.90179638775146	30.38091425913204	18.57376224502491	21.14352710809159
9	28.359369506304393	13.509960999832316	17.933315663354445	40.197353830508845
10	20.256151586782135	24.156033125801237	28.910023746287948	26.67779154112868
11	38.509284852681056	20.222344229219296	19.128067679625254	22.140303238474395
12	25.056255442984565	23.339517825943496	25.539700656133196	26.064526074938744
13	31.82124292073933	17.021193156308996	23.345197462014053	27.812366460937625
14	24.532511859621035	18.902369760535727	22.53287427449411	34.032244105349136
15	28.181813264384353	24.573215918126692	20.06358487810419	27.18138593938476
16	27.564355685856622	23.40064152841711	21.1941029150056	27.840899870720666
17	26.31159024400798	24.177669834641453	22.775475872365057	26.735264048985506
18	26.349048796187613	21.748408349605942	24.29640127440215	27.606141579804294
19	27.165158407754596	22.805226347020355	23.340329202525005	26.689286042700044
20	26.979623629449723	22.50177150553629	23.254052826024633	27.264552038989347
21	29.859740034943282	22.15680122896506	22.38060593603107	25.60285280006058
22	28.883113089667933	22.41427806416366	22.154096640360034	26.548512205808372
23	27.252246160836474	22.3835809834966	22.7255762126023	27.638596643064623
24	27.15772078909077	22.96641982787998	22.980618918056376	26.895240464972876
25	27.110390488502794	21.913388254512604	23.19360527070227	27.782615986282327
26	27.602219926327002	23.114360824574973	23.51450470868876	25.76891454040926
27	28.330565637660854	21.958690113646814	22.44970817488952	27.261036073802813
28	26.421396541372093	21.98803490001136	24.164417350476818	27.426151208139732
29	26.229370750415153	22.18154821470106	24.64028971553137	26.94879131935241
30	25.52820615456183	22.890556117508964	24.497757896046434	27.083479831882773
31	26.573259191544373	22.842008752048727	23.224572810229834	27.360159246177062
32	25.812728875810702	22.340442795246414	23.85933975582974	27.987488573113144
33	26.322679057288596	22.456469646402084	23.818230009033325	27.40262128727599
34	27.12891692044723	22.404812004046065	23.95075485067966	26.51551622482704
35	27.113635994828826	22.018461521817915	24.635150997181817	26.23275148617144
36	25.881290196948143	22.871894456134278	23.624175776622618	27.62263957029496
37	25.540647262144955	21.85740327038854	24.83840083084962	27.763548636616886
38	25.73659470657918	21.025877503772904	25.874528725435574	27.36299906421234
39	24.95253446998177	21.12878710019419	26.267640679176292	27.65103775064775
40	25.15186265017228	20.59070919722401	27.224929815925698	27.032498336678007
41	23.427957873327887	20.956775264914455	28.336380503161664	27.278886358595994
42	23.989295238301303	21.42331679928166	27.55624192004154	27.031146042375493
43	22.971017628508527	19.832342552374357	28.59480394437202	28.601835874745092
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	279.0
1	210.0
2	141.0
3	234.5
4	328.0
5	328.0
6	398.5
7	469.0
8	438.5
9	408.0
10	615.0
11	822.0
12	822.0
13	1410.5
14	1999.0
15	2872.0
16	3745.0
17	3429.0
18	3113.0
19	3113.0
20	3467.0
21	3821.0
22	3271.0
23	2721.0
24	3128.0
25	3535.0
26	3535.0
27	4264.0
28	4993.0
29	6054.0
30	7115.0
31	8586.5
32	10058.0
33	10058.0
34	12374.5
35	14691.0
36	17965.5
37	21240.0
38	23934.0
39	26628.0
40	26628.0
41	29213.5
42	31799.0
43	34316.0
44	36833.0
45	38899.0
46	40965.0
47	40965.0
48	43315.0
49	45665.0
50	48536.0
51	51407.0
52	55239.0
53	59071.0
54	59071.0
55	59405.5
56	59740.0
57	57565.0
58	55390.0
59	52567.0
60	49744.0
61	49744.0
62	47813.5
63	45883.0
64	42172.0
65	38461.0
66	33658.5
67	28856.0
68	28856.0
69	25131.0
70	21406.0
71	19880.5
72	18355.0
73	17857.0
74	17359.0
75	17359.0
76	16346.5
77	15334.0
78	12201.0
79	9068.0
80	6129.0
81	3190.0
82	3190.0
83	2514.0
84	1838.0
85	1625.5
86	1413.0
87	1229.5
88	1046.0
89	1046.0
90	739.5
91	433.0
92	257.0
93	81.0
94	55.5
95	30.0
96	30.0
97	17.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	739484.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.370759478918906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.56243996445497	44.10586602836838
2	6.324681605265615	6.371580318391541
3	1.8611581873582668	2.812438542229318
4	0.9018503599935175	1.817075502768396
5	0.5966975840877387	1.5028055244867735
6	0.4079026205448397	1.2327818874170908
7	0.2757320116357596	0.9722181587120321
8	0.22958478494241566	0.9251487985882995
9	0.17106231262850294	0.7754884744786159
>10	1.3403645494217438	13.469321823566712
>50	0.15819918263637092	5.587531303915597
>100	0.16008566644927272	16.02138706429424
>500	0.008354639158125003	2.9020946675176993
>1k	0.0018865314228024201	1.5042619052653954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2281	0.30845833040336235	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1922	0.2599109649431225	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1761	0.23813902667265283	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1641	0.2219114950424891	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1378	0.18634615488638023	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1094	0.14794099669499272	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1004	0.13577034797236992	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	991	0.1340123653791022	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	958	0.12954979418080714	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	926	0.12522245241276347	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	911	0.12319401095899302	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	903	0.12211217551698211	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	799	0.1080483147708402	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	746	0.1008811549675179	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3522943025136446E-4
2	0.0	0.0	0.0	0.0	1.3522943025136446E-4
3	0.0	0.0	0.0	0.0	1.3522943025136446E-4
4	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
5	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
6	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
7	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
8	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
9	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
10	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
11	0.0	0.0	0.0	1.3522943025136446E-4	1.3522943025136446E-4
12	0.0	0.0	0.0	5.409177210054578E-4	1.3522943025136446E-4
13	0.0	0.0	0.0	5.409177210054578E-4	1.3522943025136446E-4
14	0.0	0.0	0.0	6.761471512568224E-4	1.3522943025136446E-4
15	0.0	0.0	0.0	0.0010818354420109157	1.3522943025136446E-4
16	0.0	0.0	0.0	0.0018932120235191025	1.3522943025136446E-4
17	1.3522943025136446E-4	0.0	0.0	0.002028441453770467	1.3522943025136446E-4
18	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.002569359174775925	1.3522943025136446E-4
19	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.0029750474655300184	1.3522943025136446E-4
20	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.0036511946167868408	1.3522943025136446E-4
21	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.00486825948904912	1.3522943025136446E-4
22	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.0060853243613114005	1.3522943025136446E-4
23	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.008248995245333233	1.3522943025136446E-4
24	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.011900189862120074	1.3522943025136446E-4
25	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.016227531630163737	1.3522943025136446E-4
26	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.02812772149228381	1.3522943025136446E-4
27	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.07856829897604276	1.3522943025136446E-4
28	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.2405731564171774	1.3522943025136446E-4
29	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.45004354387654094	1.3522943025136446E-4
30	1.3522943025136446E-4	1.3522943025136446E-4	0.0	0.7214490103910294	1.3522943025136446E-4
31	1.3522943025136446E-4	1.3522943025136446E-4	0.0	1.2792704101779078	1.3522943025136446E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	25	0.0054950924	29.599998	37
TGCCGGA	25	0.0054950924	29.599998	7
TATAGAG	50	2.70084E-4	22.199999	2
GGTATCA	870	0.0	21.902298	1
TAGATAA	210	0.0	20.261906	4
TAGAGCA	55	5.140709E-4	20.181818	4
TGCATCA	605	0.0	19.876032	14
CGGTGAT	160	0.0	19.65625	14
GCATCAG	620	0.0	19.395163	15
CTAGATA	220	0.0	19.340908	3
ATACACA	280	0.0	19.160713	37
ATCAGAA	645	0.0	18.930233	17
TCTAGAT	225	0.0	18.911112	2
GCTGCAT	640	0.0	18.789062	12
GATAACC	230	0.0	18.5	6
AGATAAC	230	0.0	18.5	5
CTGCATC	640	0.0	18.5	13
GTATACT	60	9.2325703E-4	18.5	4
AGTCGGT	150	1.2732926E-11	18.5	11
TGTGCGT	50	0.007032569	18.499998	10
>>END_MODULE