FastQCFastQC Report
Fri 10 Feb 2017
ERR1631450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493451
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22390.4537431274837826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20010.4055113881621478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16810.3406619907549078No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA7710.15624651687806893No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6840.13861558695797557No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6180.1252403987427323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.12463243564203944No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC5640.11429706293026055No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5510.11166255616059144No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC5050.10234045528330067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.021.3133621
GTTCTAG450.003822760720.5555551
CTTATAC4700.019.6808537
TGTACTG1052.251545E-819.380955
AGTCGGT5550.019.011
CAGTCGG5600.018.83035710
GCAGTCG5700.018.5000029
TGTATAA609.2266337E-418.511
TACGCTA500.00702933218.59
CGCTCAT609.2266337E-418.510
TATACAG500.00702933218.55
CGCTTCG801.6136259E-518.532
GGCAGTC6000.017.8833338
GTACTAT650.001578207117.0769231
TAGGCAG650.001578207117.0769235
GGCTAAA650.001578207117.0769231
GGGCAGT6400.017.0546887
TCGGTGA6200.017.00806413
CGCCTTA4400.016.81818225
GTCGGTG6450.016.63565812
Produced by FastQC (version 0.11.5)