##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631450.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493451
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.059945161728315	34.0	33.0	34.0	31.0	34.0
2	33.1985668283173	34.0	33.0	34.0	31.0	34.0
3	33.27938538983607	34.0	34.0	34.0	31.0	34.0
4	36.51147733006925	37.0	37.0	37.0	35.0	37.0
5	36.49621340315452	37.0	37.0	37.0	35.0	37.0
6	36.543354861982245	37.0	37.0	37.0	35.0	37.0
7	36.54995126162476	37.0	37.0	37.0	35.0	37.0
8	36.550660551908905	37.0	37.0	37.0	35.0	37.0
9	38.400414630834675	39.0	39.0	39.0	37.0	39.0
10	38.364023986170864	39.0	39.0	39.0	37.0	39.0
11	38.42032339583869	39.0	39.0	39.0	37.0	39.0
12	38.38114220054271	39.0	39.0	39.0	37.0	39.0
13	38.403827330373225	39.0	39.0	39.0	37.0	39.0
14	39.9482582870437	41.0	40.0	41.0	38.0	41.0
15	39.93129611653437	41.0	40.0	41.0	38.0	41.0
16	39.90111074858497	41.0	40.0	41.0	38.0	41.0
17	39.882209175784425	41.0	40.0	41.0	38.0	41.0
18	39.87849857432653	41.0	40.0	41.0	38.0	41.0
19	39.893065370219134	41.0	40.0	41.0	38.0	41.0
20	39.88058388776191	41.0	40.0	41.0	38.0	41.0
21	39.84874283363495	41.0	40.0	41.0	38.0	41.0
22	39.789425900444016	41.0	40.0	41.0	38.0	41.0
23	39.73028730309595	41.0	40.0	41.0	38.0	41.0
24	39.69823954151476	41.0	40.0	41.0	37.0	41.0
25	39.69021848167295	41.0	40.0	41.0	37.0	41.0
26	39.59045984302393	41.0	40.0	41.0	37.0	41.0
27	39.53320795783168	41.0	40.0	41.0	37.0	41.0
28	39.457204464070394	41.0	39.0	41.0	36.0	41.0
29	39.39190314742497	41.0	39.0	41.0	36.0	41.0
30	39.307548267203835	41.0	39.0	41.0	35.0	41.0
31	39.24102899781336	41.0	39.0	41.0	35.0	41.0
32	39.1545786714385	41.0	39.0	41.0	35.0	41.0
33	39.06179742264176	41.0	39.0	41.0	35.0	41.0
34	39.00062417545004	41.0	39.0	41.0	35.0	41.0
35	38.90816109400934	41.0	39.0	41.0	35.0	41.0
36	38.84070961453113	40.0	38.0	41.0	35.0	41.0
37	38.76335846922998	40.0	38.0	41.0	35.0	41.0
38	38.67995201144592	40.0	38.0	41.0	35.0	41.0
39	38.575838330452264	40.0	38.0	41.0	35.0	41.0
40	38.464508127453385	40.0	38.0	41.0	35.0	41.0
41	38.35364605604204	40.0	38.0	41.0	35.0	41.0
42	38.2075423902272	40.0	37.0	41.0	35.0	41.0
43	37.37312316724457	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	1.0
15	4.0
16	3.0
17	3.0
18	6.0
19	18.0
20	26.0
21	50.0
22	106.0
23	178.0
24	278.0
25	438.0
26	657.0
27	960.0
28	1336.0
29	1958.0
30	2639.0
31	3432.0
32	4262.0
33	5797.0
34	8543.0
35	13176.0
36	21524.0
37	43889.0
38	93642.0
39	290523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64222587450426	16.88191938003976	12.29119000670786	27.184664738748122
2	19.82344751555879	19.274254181266226	33.14594559540866	27.756352707766325
3	20.36190016840578	20.77146464390588	28.573049806363755	30.29358538132459
4	14.614622323189131	15.101803421211022	32.974905309747065	37.30866894585278
5	16.291181900533182	35.12912122986882	31.826463012538227	16.75323385705977
6	35.2417970578639	32.91187980164191	16.149323843704845	15.696999296789347
7	31.782892323655236	29.26551977805294	19.155093413530423	19.796494484761403
8	30.478203509568324	30.35336841955939	18.4318199780728	20.73660809279949
9	28.702748601178236	13.52413917491301	18.167356029271396	39.60575619463736
10	19.22946756618185	24.762742399954604	29.594225161160885	26.41356487270266
11	37.89312413998553	21.020932169556854	19.20737824019001	21.878565450267608
12	24.375469904813244	24.848262542785406	26.260560825694952	24.5157067267064
13	32.51487989688946	17.639441403503085	23.529590577382557	26.3160881222249
14	24.3195372995495	19.47366607829349	23.17676932461379	33.03002729754322
15	28.096406735420537	25.452577864874122	20.46505124115667	25.98596415854867
16	26.98950858342571	24.094185643559342	22.606702590530773	26.309603182484175
17	25.081517719084566	25.11637427019096	23.504056127153454	26.29805188357101
18	26.18659198177732	22.47882768501837	24.989512636513048	26.34506769669126
19	25.965496067492012	24.501926229757363	24.539417287633423	24.993160415117206
20	26.88189911460307	22.633858275695054	24.670332008649286	25.813910601052587
21	28.284267333534636	22.891026667288138	23.892544548496204	24.93216145068102
22	27.97927251135371	22.496255960571567	23.256817799538354	26.267653728536374
23	26.2737333595433	22.89690364392817	23.758184703243078	27.071178293285453
24	25.544785601812542	23.429884628868926	24.803881236434826	26.221448532883713
25	25.72413471651694	22.739846509582513	24.289341798881754	27.246676975018797
26	26.23198655996239	23.46717303237809	24.2854913659107	26.015349041748827
27	26.78320643792393	22.38864649174893	24.054262733280506	26.77388433704664
28	25.445079653298908	23.01626706603087	24.609130389846207	26.929522890824014
29	24.85109970392197	23.473252663385015	25.542759058143567	26.13288857454945
30	24.37648317664773	24.43322639937907	25.571535978243027	25.618754445730175
31	25.935908529924955	24.179097823289446	23.748051984898197	26.136941661887402
32	25.002685170361396	23.72028833663322	25.017884247878712	26.25914224512667
33	25.16359273767811	22.89285055659022	25.2665411560621	26.67701554966957
34	25.921925378609025	22.860020549152804	24.85555809999372	26.36249597224446
35	25.512158248742022	23.10442171563134	25.357532966799134	26.0258870688275
36	24.180313749490832	24.064192797258492	25.499188369260573	26.2563050839901
37	25.29471011306087	22.909265560308924	25.694952487683683	26.10107183894652
38	24.239083515891142	22.01637041975799	26.785232981592905	26.959313082757962
39	24.907234963552614	21.796895740407862	27.066314588479912	26.229554707559615
40	24.16085893026866	21.59991569578337	27.140688741131335	27.098536632816632
41	22.61946981564532	21.9472652806459	28.83852702699964	26.594737876709136
42	23.32450435808216	21.95415552912042	28.45024126002379	26.271098852773626
43	21.91038218587053	21.680774788175523	28.904592350608265	27.504250675345677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	44.0
2	42.0
3	105.0
4	168.0
5	168.0
6	261.0
7	354.0
8	337.5
9	321.0
10	496.5
11	672.0
12	672.0
13	1252.5
14	1833.0
15	2985.0
16	4137.0
17	3818.0
18	3499.0
19	3499.0
20	4055.0
21	4611.0
22	3705.0
23	2799.0
24	3035.5
25	3272.0
26	3272.0
27	3832.0
28	4392.0
29	5293.0
30	6194.0
31	7263.5
32	8333.0
33	8333.0
34	9797.5
35	11262.0
36	12947.5
37	14633.0
38	16190.0
39	17747.0
40	17747.0
41	19391.0
42	21035.0
43	22677.5
44	24320.0
45	26656.5
46	28993.0
47	28993.0
48	32929.5
49	36866.0
50	37134.0
51	37402.0
52	37770.0
53	38138.0
54	38138.0
55	36853.0
56	35568.0
57	35311.5
58	35055.0
59	33995.5
60	32936.0
61	32936.0
62	31407.5
63	29879.0
64	27864.5
65	25850.0
66	23040.0
67	20230.0
68	20230.0
69	17420.0
70	14610.0
71	12569.5
72	10529.0
73	8176.5
74	5824.0
75	5824.0
76	4778.0
77	3732.0
78	3255.0
79	2778.0
80	2379.0
81	1980.0
82	1980.0
83	1676.5
84	1373.0
85	1148.0
86	923.0
87	802.0
88	681.0
89	681.0
90	497.5
91	314.0
92	198.5
93	83.0
94	54.0
95	25.0
96	25.0
97	16.0
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	493451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.48625679544693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.43746438695686	60.79334737955748
2	4.676539020106031	6.218511484093947
3	1.2470315575920874	2.487313811102811
4	0.6268131697348617	1.6669784546304034
5	0.3863525876984469	1.2843568679652184
6	0.25129433946759994	1.002457199105104
7	0.1869383178713488	0.8700180304832368
8	0.1477436959842642	0.7858340248894587
9	0.11863228700065606	0.7098675027981103
>10	0.7515706376302245	9.791760719596084
>50	0.10268886520444412	4.80437512362251
>100	0.06387491855431555	7.506683682138458
>500	0.002139351339139755	0.8753699835598754
>1k	9.168648596313235E-4	1.2031257364572938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2239	0.4537431274837826	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2001	0.4055113881621478	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1681	0.3406619907549078	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	771	0.15624651687806893	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	684	0.13861558695797557	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	618	0.1252403987427323	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	615	0.12463243564203944	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	564	0.11429706293026055	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	551	0.11166255616059144	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	505	0.10234045528330067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.026543668976251E-4	0.0	0.0	0.0
3	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
4	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
5	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
6	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
7	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
8	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
9	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
10	0.0	2.026543668976251E-4	0.0	2.026543668976251E-4	0.0
11	0.0	2.026543668976251E-4	0.0	4.053087337952502E-4	0.0
12	0.0	2.026543668976251E-4	0.0	6.079631006928753E-4	0.0
13	0.0	2.026543668976251E-4	0.0	6.079631006928753E-4	0.0
14	0.0	2.026543668976251E-4	0.0	6.079631006928753E-4	0.0
15	0.0	2.026543668976251E-4	0.0	6.079631006928753E-4	0.0
16	0.0	2.026543668976251E-4	0.0	0.002229198035873876	0.0
17	0.0	2.026543668976251E-4	0.0	0.0028371611365667513	0.0
18	0.0	2.026543668976251E-4	0.0	0.0030398155034643765	0.0
19	0.0	2.026543668976251E-4	0.0	0.003445124237259627	0.0
20	0.0	2.026543668976251E-4	0.0	0.004255741704850127	0.0
21	2.026543668976251E-4	2.026543668976251E-4	0.0	0.004255741704850127	0.0
22	2.026543668976251E-4	2.026543668976251E-4	0.0	0.007295557208314503	0.0
23	2.026543668976251E-4	2.026543668976251E-4	0.0	0.012564570747652756	0.0
24	2.026543668976251E-4	2.026543668976251E-4	0.0	0.017630929920093383	0.0
25	2.026543668976251E-4	2.026543668976251E-4	0.0	0.020062782322864885	0.0
26	2.026543668976251E-4	2.026543668976251E-4	0.0	0.027358339531179388	0.0
27	2.026543668976251E-4	2.026543668976251E-4	2.026543668976251E-4	0.05228482665958727	2.026543668976251E-4
28	2.026543668976251E-4	2.026543668976251E-4	2.026543668976251E-4	0.15969164111532858	2.026543668976251E-4
29	2.026543668976251E-4	2.026543668976251E-4	2.026543668976251E-4	0.30560278528161866	2.026543668976251E-4
30	2.026543668976251E-4	2.026543668976251E-4	2.026543668976251E-4	0.4918421484605361	2.026543668976251E-4
31	2.026543668976251E-4	2.026543668976251E-4	2.026543668976251E-4	1.007597512214992	2.026543668976251E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1085	0.0	21.313362	1
GTTCTAG	45	0.0038227607	20.555555	1
CTTATAC	470	0.0	19.68085	37
TGTACTG	105	2.251545E-8	19.38095	5
AGTCGGT	555	0.0	19.0	11
CAGTCGG	560	0.0	18.830357	10
GCAGTCG	570	0.0	18.500002	9
TGTATAA	60	9.2266337E-4	18.5	11
TACGCTA	50	0.007029332	18.5	9
CGCTCAT	60	9.2266337E-4	18.5	10
TATACAG	50	0.007029332	18.5	5
CGCTTCG	80	1.6136259E-5	18.5	32
GGCAGTC	600	0.0	17.883333	8
GTACTAT	65	0.0015782071	17.076923	1
TAGGCAG	65	0.0015782071	17.076923	5
GGCTAAA	65	0.0015782071	17.076923	1
GGGCAGT	640	0.0	17.054688	7
TCGGTGA	620	0.0	17.008064	13
CGCCTTA	440	0.0	16.818182	25
GTCGGTG	645	0.0	16.635658	12
>>END_MODULE