FastQCFastQC Report
Fri 10 Feb 2017
ERR1631449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065133
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA37990.1839590960969584No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC32450.15713273672930508No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC26570.1286599942957669No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC26080.12628726575963872No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC25770.12478615178780253No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22020.10662751503171951No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT20770.10057463611302517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.025.91
TTGTACG656.905537E-519.9230774
CTAGACA3750.019.7333324
CTTATAC21000.019.20476337
CTAGAAC4650.019.0967753
ATCGTAC609.240214E-418.510
TAGAACT5450.018.3302754
TCGTTTA4850.018.30927730
TAACGCC5500.016.4818174
GTTCTAG3850.016.3376621
GTCTAGA4500.016.0333331
GTATCAA15900.015.7075462
TATACAC6250.015.68837
ATAACGC5800.015.6293113
AATAACG5800.015.6293112
CGTTTAT5700.015.57894731
GTATTAA1455.3516487E-815.3103451
TTAGAAC1701.4879333E-915.2352933
TAGACGT1101.45290305E-515.1363644
GAATAAC6150.015.0406511
Produced by FastQC (version 0.11.5)