##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631449.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2065133
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.087260239413155	34.0	33.0	34.0	31.0	34.0
2	33.230111571506534	34.0	33.0	34.0	31.0	34.0
3	33.32355494779271	34.0	34.0	34.0	31.0	34.0
4	36.54332820210611	37.0	37.0	37.0	35.0	37.0
5	36.53039392620233	37.0	37.0	37.0	35.0	37.0
6	36.57679626445367	37.0	37.0	37.0	35.0	37.0
7	36.57894382589402	37.0	37.0	37.0	35.0	37.0
8	36.57096564724887	37.0	37.0	37.0	35.0	37.0
9	38.42688437015921	39.0	39.0	39.0	37.0	39.0
10	38.38395735286783	39.0	39.0	39.0	37.0	39.0
11	38.4421691000047	39.0	39.0	39.0	37.0	39.0
12	38.39747222091749	39.0	39.0	39.0	37.0	39.0
13	38.42564135094447	39.0	39.0	39.0	37.0	39.0
14	39.991748231227724	41.0	40.0	41.0	38.0	41.0
15	39.99306582191075	41.0	40.0	41.0	38.0	41.0
16	39.972979948506946	41.0	40.0	41.0	38.0	41.0
17	39.957813370857956	41.0	40.0	41.0	38.0	41.0
18	39.93210945735699	41.0	40.0	41.0	38.0	41.0
19	39.943458363214376	41.0	40.0	41.0	38.0	41.0
20	39.917916182638116	41.0	40.0	41.0	38.0	41.0
21	39.88802803499823	41.0	40.0	41.0	38.0	41.0
22	39.842437750982626	41.0	40.0	41.0	38.0	41.0
23	39.78816909128855	41.0	40.0	41.0	38.0	41.0
24	39.76579813503537	41.0	40.0	41.0	38.0	41.0
25	39.74447602164122	41.0	40.0	41.0	38.0	41.0
26	39.67077519946657	41.0	40.0	41.0	37.0	41.0
27	39.62267418127549	41.0	40.0	41.0	37.0	41.0
28	39.54738653636352	41.0	40.0	41.0	37.0	41.0
29	39.50194200567228	41.0	40.0	41.0	37.0	41.0
30	39.43798535009609	41.0	39.0	41.0	36.0	41.0
31	39.40614091198969	41.0	39.0	41.0	36.0	41.0
32	39.34383645024316	41.0	39.0	41.0	36.0	41.0
33	39.26991869288806	41.0	39.0	41.0	35.0	41.0
34	39.22135378205665	41.0	39.0	41.0	35.0	41.0
35	39.13764149815048	41.0	39.0	41.0	35.0	41.0
36	39.08086839927501	41.0	39.0	41.0	35.0	41.0
37	39.023109407481265	41.0	39.0	41.0	35.0	41.0
38	38.95880555877031	41.0	39.0	41.0	35.0	41.0
39	38.88564223224364	40.0	38.0	41.0	35.0	41.0
40	38.794778350837454	40.0	38.0	41.0	35.0	41.0
41	38.7165707971351	40.0	38.0	41.0	35.0	41.0
42	38.596675371513605	40.0	38.0	41.0	35.0	41.0
43	37.795295024581954	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	3.0
17	10.0
18	28.0
19	46.0
20	106.0
21	177.0
22	356.0
23	640.0
24	970.0
25	1554.0
26	2233.0
27	3317.0
28	4597.0
29	6213.0
30	8657.0
31	11480.0
32	14955.0
33	20990.0
34	32069.0
35	49050.0
36	83014.0
37	160197.0
38	396680.0
39	1267787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86986213478745	17.254724029880883	11.527586843074998	27.347826992256675
2	19.83029664433235	19.17150130282166	32.711985136066296	28.286216916779694
3	20.294092438598383	20.40459379613807	28.222056400241534	31.079257365022013
4	14.700505972254572	14.5126246106183	33.04639459056632	37.740474826560806
5	16.884965762495685	34.227964978526806	32.103017093814294	16.784052165163214
6	36.77850288577055	33.36511498290909	14.893859136433344	14.962522994887012
7	32.055514100060385	28.578401487942905	19.504554912443897	19.861529499552812
8	29.383143845941156	30.929775467245936	18.828085164490616	20.858995522322292
9	27.730514209012203	13.498404219001875	18.18914326583324	40.58193830615268
10	18.601029570492557	24.40225399526326	30.483411964265738	26.51330446997845
11	39.32448902806745	19.937456812708916	19.62285237803086	21.115201781192784
12	24.142609701166947	23.663318536869056	26.636492661731715	25.557579100232285
13	31.87949638110475	17.1496944748837	23.61363650670441	27.357172637307137
14	24.351458235377578	18.746492356666618	22.471288774137065	34.43076063381874
15	27.46007157892494	25.424706302209106	20.14906545970647	26.966156659159484
16	27.760294373292183	23.798709332522407	21.70499430303036	26.73600199115505
17	26.562550692860942	24.07147626811445	22.53486821429903	26.83110482472558
18	26.91201971011068	22.215663591642766	23.996033185271845	26.87628351297471
19	26.81565787772507	23.24615412179264	23.04079204583918	26.897395954643116
20	27.045134623290608	22.346454199317915	23.125048120387405	27.48336305700408
21	28.563148233067796	23.02108387207991	22.695100025034705	25.720667869817586
22	28.434004008458537	22.42509320222959	22.470078198353328	26.670824590958546
23	27.012206961972907	22.24665433170648	23.180686183408042	27.560452522912566
24	26.41762056003173	23.245766737541842	22.919540775339893	27.417071927086532
25	27.137477344074206	22.220990125091216	23.076189281755703	27.56534324907887
26	26.98731752385924	23.37655734521699	23.008639153023076	26.627485977900694
27	27.513288490378102	22.34906904301079	23.01909852779458	27.11854393881653
28	26.47248385455077	22.67742561859212	23.69004805017401	27.1600424766831
29	25.829522844291386	23.044617465315792	23.91923425755145	27.20662543284137
30	25.36906823918847	23.865678384878844	24.150551078308276	26.614702297624415
31	27.08304985683731	23.128873539864017	22.51850122970288	27.269575373595796
32	26.244895607207862	22.731223606421473	23.108874827916654	27.91500595845401
33	25.60183775088578	22.474387848143436	23.970126863499832	27.953647537470953
34	27.003732931486734	22.135378205665205	23.93700551005674	26.92388335279132
35	26.51867942645825	22.217503666834048	24.6913394924201	26.572477414287604
36	25.56585943859306	22.945253404986506	24.367873643005076	27.12101351341536
37	26.272303042951712	21.7063985709395	24.811089648947547	27.21020873716124
38	25.051897383848885	21.307925445964013	25.80913674809322	27.831040422093878
39	25.738003315040725	21.042131426886307	25.946077080749763	27.273788177323205
40	24.944204562127474	21.507960988469023	26.655958720334233	26.891875729069266
41	24.22599416115088	21.485202163734733	27.52122986751943	26.767573807594957
42	24.894570954994183	21.47450067380648	27.58258184823931	26.04834652296002
43	23.197150014066892	20.641963495813588	27.807991059171496	28.35289543094803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	65.5
2	80.0
3	175.5
4	271.0
5	271.0
6	333.5
7	396.0
8	360.0
9	324.0
10	475.0
11	626.0
12	626.0
13	1095.5
14	1565.0
15	2379.0
16	3193.0
17	3242.0
18	3291.0
19	3291.0
20	4029.5
21	4768.0
22	4955.0
23	5142.0
24	6388.0
25	7634.0
26	7634.0
27	9717.5
28	11801.0
29	15104.0
30	18407.0
31	22608.5
32	26810.0
33	26810.0
34	34219.5
35	41629.0
36	51240.5
37	60852.0
38	70747.0
39	80642.0
40	80642.0
41	88796.5
42	96951.0
43	104816.5
44	112682.0
45	123297.5
46	133913.0
47	133913.0
48	142972.0
49	152031.0
50	156134.5
51	160238.0
52	166138.0
53	172038.0
54	172038.0
55	167845.0
56	163652.0
57	158815.5
58	153979.0
59	150635.5
60	147292.0
61	147292.0
62	138627.0
63	129962.0
64	121771.5
65	113581.0
66	101472.5
67	89364.0
68	89364.0
69	75356.0
70	61348.0
71	52595.0
72	43842.0
73	33477.5
74	23113.0
75	23113.0
76	18602.5
77	14092.0
78	11846.5
79	9601.0
80	8655.0
81	7709.0
82	7709.0
83	6252.0
84	4795.0
85	4085.5
86	3376.0
87	2935.5
88	2495.0
89	2495.0
90	1835.0
91	1175.0
92	734.0
93	293.0
94	190.0
95	87.0
96	87.0
97	59.5
98	32.0
99	21.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2065133.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.38368945851007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.04798068833583	42.850310480744746
2	7.682584357590737	7.741538890233176
3	2.4315982030055	3.675386662443008
4	1.2061161541720242	2.430743270507829
5	0.7301205058115139	1.8393082416048807
6	0.4877008984521115	1.4743302369748528
7	0.3452048703317691	1.2174886490452765
8	0.26587864430203695	1.0716757638530794
9	0.212772928753865	0.9648256650761182
>10	1.3353600754964055	12.685257468007421
>50	0.13054055317255114	4.585398624346195
>100	0.10624428007498031	10.789248514984848
>500	0.0113545439735522	3.846100112424091
>1k	0.006543296527131777	4.828387419754447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3799	0.1839590960969584	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3245	0.15713273672930508	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2657	0.1286599942957669	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2608	0.12628726575963872	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2577	0.12478615178780253	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2202	0.10662751503171951	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2077	0.10057463611302517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.936921253982189E-4	0.0	0.0	0.0
6	0.0	1.936921253982189E-4	0.0	0.0	0.0
7	0.0	1.936921253982189E-4	0.0	0.0	0.0
8	0.0	1.936921253982189E-4	0.0	0.0	0.0
9	0.0	2.4211515674777364E-4	0.0	0.0	0.0
10	0.0	2.4211515674777364E-4	0.0	0.0	0.0
11	0.0	2.9053818809732836E-4	0.0	0.0	0.0
12	0.0	2.9053818809732836E-4	0.0	0.0	0.0
13	0.0	2.9053818809732836E-4	0.0	0.0	0.0
14	0.0	2.9053818809732836E-4	0.0	4.842303134955473E-5	0.0
15	0.0	2.9053818809732836E-4	0.0	9.684606269910945E-5	0.0
16	0.0	2.9053818809732836E-4	0.0	2.9053818809732836E-4	0.0
17	0.0	2.9053818809732836E-4	0.0	7.263454702433209E-4	0.0
18	0.0	3.3896121944688307E-4	0.0	7.747685015928756E-4	0.0
19	0.0	3.3896121944688307E-4	0.0	0.0010168836583406492	0.0
20	0.0	3.3896121944688307E-4	0.0	0.001210575783738868	0.0
21	0.0	3.3896121944688307E-4	0.0	0.0016463830658848607	0.0
22	0.0	3.3896121944688307E-4	0.0	0.00266326672422551	0.0
23	0.0	6.294994075442114E-4	0.0	0.0035833043198670498	0.0
24	0.0	6.294994075442114E-4	0.0	0.005278110417101465	0.0
25	0.0	6.294994075442114E-4	0.0	0.0068276474202872165	0.0
26	0.0	6.294994075442114E-4	0.0	0.010071990520707383	0.0
27	0.0	6.779224388937661E-4	0.0	0.029828587311325712	0.0
28	0.0	7.747685015928756E-4	0.0	0.12260711537707257	0.0
29	0.0	8.71614564291985E-4	0.0	0.25286506970737477	0.0
30	0.0	8.71614564291985E-4	0.0	0.42544475343718785	0.0
31	0.0	8.71614564291985E-4	0.0	0.9568875224985509	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	25.9	1
TTGTACG	65	6.905537E-5	19.923077	4
CTAGACA	375	0.0	19.733332	4
CTTATAC	2100	0.0	19.204763	37
CTAGAAC	465	0.0	19.096775	3
ATCGTAC	60	9.240214E-4	18.5	10
TAGAACT	545	0.0	18.330275	4
TCGTTTA	485	0.0	18.309277	30
TAACGCC	550	0.0	16.481817	4
GTTCTAG	385	0.0	16.337662	1
GTCTAGA	450	0.0	16.033333	1
GTATCAA	1590	0.0	15.707546	2
TATACAC	625	0.0	15.688	37
ATAACGC	580	0.0	15.629311	3
AATAACG	580	0.0	15.629311	2
CGTTTAT	570	0.0	15.578947	31
GTATTAA	145	5.3516487E-8	15.310345	1
TTAGAAC	170	1.4879333E-9	15.235293	3
TAGACGT	110	1.45290305E-5	15.136364	4
GAATAAC	615	0.0	15.040651	1
>>END_MODULE