FastQCFastQC Report
Fri 10 Feb 2017
ERR1631448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683440
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.2399625424324008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15500.22679386632330564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12140.17763080884935034No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10880.1591946622966171No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9970.14587966756408755No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8670.12685824651761676No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT7840.11471380077256234No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7820.11442116352569356No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC7290.10666627648367084No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT6930.10139880604003278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT250.00549480429.5999981
CCTAGTC358.8657E-426.4285722
GGACCGT358.8657E-426.4285726
GGTATCA8250.024.6666641
CTAGACA1800.023.638894
TCTAGAC1650.023.5454543
GCTTAGA400.001930390823.1251
CAAGACG2500.022.1999994
AAGACGG2350.022.0425555
AGACGGA2300.021.7173926
ACGGACC2350.021.255328
GTTCTAG2050.020.7560961
GATTTAT450.003824245220.5555551
TCGTCCG450.003824245220.55555533
GTATAGA450.003824245220.5555551
CTCTAGT1800.020.55555527
TTTATAC555.140162E-420.1818183
CTAGTTT1750.020.08571429
GTGTAAG656.896898E-519.9230771
TCTAGTT1950.018.9743628
Produced by FastQC (version 0.11.5)