FastQCFastQC Report
Fri 10 Feb 2017
ERR1631447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65258
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14802.267921174415397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14292.18976983664838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10641.6304514389040423No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5150.7891752735296822No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.7845781360139753No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3490.5348003309939011No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.3340586594747004No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2150.32946152195899353No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.32792914278709123No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1890.28961966348953383No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1840.2819577676300224No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.2773606301143155No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1740.2666339759109994No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1710.2620368383952925No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1620.24824542584817189No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.2375187716448558No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1520.23292163412914893No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1490.228324496613442No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1490.228324496613442No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1460.22372735909773514No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1420.21759784241012597No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1380.21146832572251678No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.20993594655061448No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1290.19767691317539612No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.18388550062827547No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1180.18082074228447087No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.1746912255968617No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1110.1700940880811548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.16089981304974105No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1040.15936743387783872No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA920.1409788838150112No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA910.1394465046431089No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT880.134849367127402No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA820.12565509209598824No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT820.12565509209598824No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC760.11646081706457447No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.11646081706457447No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC750.11492843789267215No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA750.11492843789267215ABI Solid3 Adapter B (95% over 23bp)
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT740.11339605872076987No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.11033130037696529No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG680.10420178368935609No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGCGG680.10420178368935609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC453.932626E-628.77777937
TGGGAAT358.7796786E-426.4285726
CCGCTTA809.367795E-1025.437525
CGCTTAT809.367795E-1025.437526
CTCCGCT851.891749E-923.94117523
GCTTATT851.891749E-923.94117527
CCTCCGC903.6634447E-922.61111322
TCCGCTT903.6634447E-922.61111324
TGGGCAG3700.022.06
GGGCAGT3650.021.794527
TCCTCCG956.8394E-921.42105321
GCAGTCG3550.021.3661989
AGTCGGT3600.021.06944511
CAGTCGG3650.020.7808210
GGCAGTC3650.020.780828
GGTATCA7800.018.974361
GGTGATT4100.018.95122115
GTGATTC4150.018.7228916
TATACAC609.1101776E-418.537
TTCCTCC1103.6887286E-818.520
Produced by FastQC (version 0.11.5)