FastQCFastQC Report
Fri 10 Feb 2017
ERR1631446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40408
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7461.846169075430608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6571.6259156602652942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5341.3215204909918827No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.5642447040190062No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1610.3984359532765789No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.2697485646406652No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT870.21530390021777865No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA850.2103543852702435No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT830.2054048703227084No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.20293011284894083No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC780.19303108295387053No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA750.1856068105325678No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA710.1757077806374975No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA710.1757077806374975No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC680.1682835082161948No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT650.16085923579489209No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.16085923579489209No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA640.15838447832112454No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA640.15838447832112454No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC630.15590972084735696No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA620.1534349633735894No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.1460106909522867No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT590.1460106909522867No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.13858641853098397No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG520.12868738863591367No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT520.12868738863591367No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC510.12621263116214612No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT500.12373787368837855No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT490.12126311621461097No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG470.11631360126707582No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.10641457137200554No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC410.10146505642447042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCC250.005434575529.59999819
GGTCGGC250.005434575529.59999815
TGGGCGA400.001899295123.1256
GCAGTCG1004.9112714E-1022.1999999
CAGTCGG1059.0221874E-1021.14285910
GGCAGTC1059.0221874E-1021.1428598
TCGGTGA1001.1999873E-820.34999813
AGTCGGT1001.1999873E-820.34999811
GGGCAGT1101.6116246E-920.1818187
CTTATAC555.026045E-420.18181837
AGCAGTG656.693926E-519.92307725
CGGTGAT1052.1027518E-819.38095314
GTCGGTG1052.1027518E-819.38095312
TGGGCAG1253.7289283E-1019.246
GCAGTGG609.0283004E-418.526
ATTCCTC1204.7530193E-918.519
AAGCAGT701.18267824E-418.524
CTCGCCT1002.7084934E-718.49999823
GGTATCA3150.018.206351
GGTGATT1257.885319E-917.7615
Produced by FastQC (version 0.11.5)