##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631446.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40408 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.90437537121362 34.0 33.0 34.0 31.0 34.0 2 33.01692734112057 34.0 33.0 34.0 31.0 34.0 3 33.061819441694716 34.0 33.0 34.0 31.0 34.0 4 36.356959018016234 37.0 37.0 37.0 35.0 37.0 5 36.34532765788953 37.0 37.0 37.0 35.0 37.0 6 36.448970500890916 37.0 37.0 37.0 35.0 37.0 7 36.45144525836468 37.0 37.0 37.0 35.0 37.0 8 36.4751039398139 37.0 37.0 37.0 35.0 37.0 9 38.28603246881806 39.0 39.0 39.0 37.0 39.0 10 38.24121461096812 39.0 39.0 39.0 37.0 39.0 11 38.30093050881014 39.0 39.0 39.0 37.0 39.0 12 38.29655018808157 39.0 39.0 39.0 37.0 39.0 13 38.30516234409028 39.0 39.0 39.0 37.0 39.0 14 39.720872104533754 41.0 40.0 41.0 37.0 41.0 15 39.68832904375371 41.0 40.0 41.0 37.0 41.0 16 39.617649970302914 41.0 39.0 41.0 37.0 41.0 17 39.637547020392 41.0 39.0 41.0 37.0 41.0 18 39.71733320134627 41.0 40.0 41.0 38.0 41.0 19 39.747500494951495 41.0 40.0 41.0 38.0 41.0 20 39.75843892298555 41.0 40.0 41.0 38.0 41.0 21 39.74886161156207 41.0 40.0 41.0 37.0 41.0 22 39.65553850722629 41.0 40.0 41.0 37.0 41.0 23 39.56872401504653 41.0 40.0 41.0 37.0 41.0 24 39.52781627400515 41.0 40.0 41.0 36.0 41.0 25 39.52685111859038 41.0 40.0 41.0 36.0 41.0 26 39.37435656305682 41.0 39.0 41.0 36.0 41.0 27 39.281231439318944 41.0 39.0 41.0 35.0 41.0 28 39.162393585428624 41.0 39.0 41.0 35.0 41.0 29 39.0551128489408 41.0 39.0 41.0 35.0 41.0 30 38.87985052464858 40.0 38.0 41.0 35.0 41.0 31 38.66343298356761 40.0 38.0 41.0 35.0 41.0 32 38.49443179568402 40.0 37.0 41.0 35.0 41.0 33 38.34970797861809 40.0 37.0 41.0 35.0 41.0 34 38.24260047515344 40.0 37.0 41.0 35.0 41.0 35 38.070010888932885 40.0 36.0 41.0 35.0 41.0 36 37.91246782815284 40.0 36.0 41.0 35.0 41.0 37 37.7376509602059 40.0 35.0 41.0 34.0 41.0 38 37.56751138388438 40.0 35.0 41.0 34.0 41.0 39 37.32493565630568 40.0 35.0 41.0 33.0 41.0 40 37.099411007721244 40.0 35.0 41.0 33.0 41.0 41 36.829786180954265 40.0 35.0 41.0 33.0 41.0 42 36.538136012670755 40.0 35.0 41.0 31.0 41.0 43 35.67833102355969 39.0 35.0 41.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 2.0 17 0.0 18 2.0 19 1.0 20 7.0 21 6.0 22 11.0 23 20.0 24 39.0 25 38.0 26 70.0 27 139.0 28 198.0 29 320.0 30 369.0 31 512.0 32 573.0 33 739.0 34 1034.0 35 1416.0 36 2150.0 37 5344.0 38 7864.0 39 19553.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.612452979607994 16.6056226489804 14.640665214808948 24.141259156602654 2 19.60997822213423 21.062660859235795 33.32260938428034 26.004751534349634 3 19.8871510591962 24.170956246287865 29.560978024153634 26.380914670362305 4 13.034547614333794 18.355276182934073 32.78063749752525 35.829538705206886 5 13.183033062759849 40.13066719461493 30.98148881409622 15.704810928529003 6 28.79132844981192 33.948723025143536 19.760938428034052 17.49901009701049 7 28.97198574539695 32.72371807562859 19.18679469411998 19.117501484854486 8 33.2953870520689 29.06355177192635 17.766283904177392 19.87477727182736 9 29.711938230053452 13.168184517917245 18.496337358938824 38.623539893090474 10 21.339833696297763 27.15303900217779 25.88348841813502 25.623638883389425 11 33.89675311819442 25.747376757077806 17.34062561868937 23.015244506038407 12 23.841813502276775 30.016333399326868 25.019798059790137 21.122055038606216 13 33.34240744407048 19.248663630964167 24.821817461888735 22.587111463076617 14 23.28746782815284 21.824886161156208 25.769649574341713 29.117996436349237 15 29.751534349633737 25.836468026133442 21.973371609582262 22.438626014650566 16 23.705701841219557 25.648386458127103 26.24480300930509 24.40110869134825 17 20.723619085329638 29.573351811522468 25.92555929518907 23.77746980795882 18 23.08701247277767 23.332013462680656 28.630469214017022 24.95050485052465 19 22.609384280340528 27.80390021777866 29.42486636309642 20.161849138784397 20 24.445654325876063 23.468125123737874 30.699366462086715 21.386854088299348 21 26.6481884775292 23.3666600673134 28.94476341318551 21.040388041971887 22 26.24480300930509 23.13650762225302 26.87834092258959 23.74034844585231 23 22.928627994456544 25.190556325480102 27.87319342704415 24.007622253019203 24 20.99584240744407 25.692932092654917 29.73421104731736 23.577014452583647 25 21.889229855474163 24.740150465254406 28.33844783211245 25.032171847158978 26 23.633933874480302 24.86883785389032 28.197386656107703 23.29984161552168 27 22.376757077806374 24.485250445456344 27.685111859037814 25.452880617699464 28 20.94634725796872 25.48010294991091 29.810928529004162 23.762621263116216 29 21.334884181350226 25.28707186695704 30.936943179568406 22.44110077212433 30 20.24351613541873 26.79172441100772 31.90457335181152 21.060186101762028 31 21.75064343694318 27.341120570184124 28.464660463274598 22.4435755295981 32 20.83250841417541 26.749653533953673 31.288358740843396 21.12947931102752 33 20.886953078598296 25.35389031874876 31.181944169471393 22.577212433181547 34 22.42130271233419 26.14828746782815 28.04890120768165 23.38150861215601 35 20.93397347059988 26.97485646406652 29.216986735299937 22.874183330033656 36 18.996238368639872 27.25945357354979 29.843100376163136 23.9012076816472 37 20.238566620471193 26.581370025737478 30.320728568600273 22.859334785191052 38 20.619679271431398 23.690853296376954 31.535834488220154 24.15363294397149 39 20.72114432785587 24.24519897050089 32.04068501286874 22.9929716887745 40 20.090081172045142 24.10661255196991 30.931993664620865 24.871312611364086 41 17.944466442288658 25.00247475747377 32.72124331815482 24.331815482082757 42 18.251336369035833 24.472876658087507 32.16689764403088 25.108889328845773 43 16.957038210255394 25.044545634527815 33.30033656701643 24.698079588200354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 5.5 2 9.0 3 25.5 4 42.0 5 42.0 6 76.0 7 110.0 8 111.0 9 112.0 10 163.5 11 215.0 12 215.0 13 414.0 14 613.0 15 1012.5 16 1412.0 17 1227.0 18 1042.0 19 1042.0 20 1184.0 21 1326.0 22 901.0 23 476.0 24 414.5 25 353.0 26 353.0 27 363.5 28 374.0 29 454.5 30 535.0 31 605.5 32 676.0 33 676.0 34 761.0 35 846.0 36 971.5 37 1097.0 38 1249.0 39 1401.0 40 1401.0 41 1566.5 42 1732.0 43 1993.0 44 2254.0 45 2603.5 46 2953.0 47 2953.0 48 3647.0 49 4341.0 50 4059.5 51 3778.0 52 3367.0 53 2956.0 54 2956.0 55 2518.5 56 2081.0 57 1836.0 58 1591.0 59 1576.5 60 1562.0 61 1562.0 62 1597.5 63 1633.0 64 1489.5 65 1346.0 66 1245.0 67 1144.0 68 1144.0 69 978.0 70 812.0 71 709.0 72 606.0 73 476.5 74 347.0 75 347.0 76 290.5 77 234.0 78 192.0 79 150.0 80 119.5 81 89.0 82 89.0 83 78.5 84 68.0 85 55.5 86 43.0 87 34.0 88 25.0 89 25.0 90 18.5 91 12.0 92 11.0 93 10.0 94 5.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 40408.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.15808750742428 #Duplication Level Percentage of deduplicated Percentage of total 1 85.34233365477338 54.75400910710751 2 7.583413693346191 9.730746386854088 3 2.889103182256509 5.560780043555732 4 1.3461909353905497 3.4547614333795287 5 0.7791706846673095 2.4995050485052466 6 0.4513018322082931 1.7372797465848349 7 0.3240115718418515 1.4551573945753316 8 0.2391513982642237 1.2274797069887151 9 0.13886210221793635 0.801821421500693 >10 0.802314368370299 9.258067709364482 >50 0.08100289296046287 3.49435755295981 >100 0.011571841851494695 1.2324292219362503 >500 0.011571841851494695 4.793605226687784 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 1.846169075430608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 657 1.6259156602652942 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 534 1.3215204909918827 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.5642447040190062 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA 161 0.3984359532765789 No Hit CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.2697485646406652 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT 87 0.21530390021777865 No Hit CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA 85 0.2103543852702435 No Hit TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT 83 0.2054048703227084 No Hit CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.20293011284894083 No Hit GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC 78 0.19303108295387053 No Hit GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA 75 0.1856068105325678 No Hit CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA 71 0.1757077806374975 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 71 0.1757077806374975 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC 68 0.1682835082161948 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT 65 0.16085923579489209 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65 0.16085923579489209 No Hit TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA 64 0.15838447832112454 No Hit CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA 64 0.15838447832112454 No Hit GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC 63 0.15590972084735696 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA 62 0.1534349633735894 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.1460106909522867 No Hit TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT 59 0.1460106909522867 No Hit GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.13858641853098397 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG 52 0.12868738863591367 No Hit CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT 52 0.12868738863591367 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC 51 0.12621263116214612 No Hit GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT 50 0.12373787368837855 No Hit GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT 49 0.12126311621461097 No Hit ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG 47 0.11631360126707582 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.10641457137200554 No Hit GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC 41 0.10146505642447042 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.002474757473767571 0.0 17 0.0 0.0 0.0 0.002474757473767571 0.0 18 0.0 0.0 0.0 0.002474757473767571 0.0 19 0.0 0.0 0.0 0.007424272421302712 0.0 20 0.0 0.0 0.0 0.007424272421302712 0.0 21 0.0 0.0 0.0 0.009899029895070284 0.0 22 0.0 0.0 0.0 0.019798059790140567 0.0 23 0.0 0.0 0.0 0.03217184715897842 0.0 24 0.0 0.0 0.0 0.039596119580281135 0.0 25 0.0 0.0 0.0 0.047020392001583845 0.0 26 0.0 0.0 0.0 0.05691942189665413 0.0 27 0.0 0.0 0.0 0.11383884379330826 0.0 28 0.0 0.0 0.0 0.2722233221144328 0.0 29 0.0 0.0 0.0 0.5271233419124925 0.0 30 0.0 0.0 0.0 0.8735893882399525 0.0 31 0.0 0.0 0.0 1.5887942981587804 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTCC 25 0.0054345755 29.599998 19 GGTCGGC 25 0.0054345755 29.599998 15 TGGGCGA 40 0.0018992951 23.125 6 GCAGTCG 100 4.9112714E-10 22.199999 9 CAGTCGG 105 9.0221874E-10 21.142859 10 GGCAGTC 105 9.0221874E-10 21.142859 8 TCGGTGA 100 1.1999873E-8 20.349998 13 AGTCGGT 100 1.1999873E-8 20.349998 11 GGGCAGT 110 1.6116246E-9 20.181818 7 CTTATAC 55 5.026045E-4 20.181818 37 AGCAGTG 65 6.693926E-5 19.923077 25 CGGTGAT 105 2.1027518E-8 19.380953 14 GTCGGTG 105 2.1027518E-8 19.380953 12 TGGGCAG 125 3.7289283E-10 19.24 6 GCAGTGG 60 9.0283004E-4 18.5 26 ATTCCTC 120 4.7530193E-9 18.5 19 AAGCAGT 70 1.18267824E-4 18.5 24 CTCGCCT 100 2.7084934E-7 18.499998 23 GGTATCA 315 0.0 18.20635 1 GGTGATT 125 7.885319E-9 17.76 15 >>END_MODULE