FastQCFastQC Report
Fri 10 Feb 2017
ERR1631445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941895
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.1516092558087685No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT12810.13600242065198348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12080.12825208754691342No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA11510.12220045758816005No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11280.11975857181532974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9730.10330238508538638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.025.2539671
CTAGAAC3100.023.8709683
TCTAATA400.001930929723.1252
TAGAACT3400.022.8529424
GTCTAGA3200.022.5468751
TGGAACG450.003825303420.5555555
CATACAC759.262858E-619.7333343
ACTGATC3100.019.6935488
CAAGACG1900.019.4736844
TGGACTA500.007033961418.55
ACGGACC1601.8189894E-1218.58
TCTAGAC2450.018.1224483
GTATTAA1156.4012056E-817.6956521
AGAACTG4400.017.659095
AACGGAG3300.017.37878825
CTTATAC9750.017.26666637
TACTATA752.066767E-417.2666662
CTAGACA2500.017.024
ACGGAGT3400.016.86764726
AAGACGG1801.0913936E-1116.4444455
Produced by FastQC (version 0.11.5)