FastQCFastQC Report
Fri 10 Feb 2017
ERR1631444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136234
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19921.4621900553459488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13600.9982823671036599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13140.9645169340986831No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA10080.7399034015003597No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT8730.6408091959422758No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT8080.5930971710439391No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT7280.5343746788613709No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT6440.47271606206967426No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC5650.4147276010393881No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC5640.413993569887106No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA5410.39711085338461766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.38169619918669345No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT4590.3369202988974852No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT4420.32444176930868945No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA4400.32297370700412525No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA4230.3104951774153295No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA4200.3082930839584832No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT4040.29654858552196955No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT3980.29214439860827696No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA3810.2796658690194812No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3780.27746377556263485No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA3770.27672974441035275No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA3680.27012346403981385No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT3640.26718733943068546No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT3500.256910903298736No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA3490.2561768721464539No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA3260.23929415564396553No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT3220.23635803103483713No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTA3210.235623999882555No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2890.2121350030095277No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT2880.21140097185724563No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT2850.2091988784003993No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT2850.2091988784003993No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT2760.2025925980298604No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC2720.19965647342073198No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT2710.19892244226844988No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC2670.19598631765932145No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA2640.19378422420247515No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT2610.19158213074562883No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG2610.19158213074562883No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.1827737569182436No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC2380.17469941424314048No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTT2380.17469941424314048No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA2360.17323135193857628No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATA2340.17176328963401205No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2320.17029522732944785No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTT2320.17029522732944785No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT2190.16075282234978053No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT2170.1592847600452163No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC2140.15708266658837003No Hit
TATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATACA2100.1541465419792416No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT2060.15121041737011318No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA2040.14974235506554898No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA2030.14900832391326688No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA2020.14827429276098478No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT2010.14754026160870268No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT1970.14460413699957428No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCT1970.14460413699957428No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT1910.14019995008588162No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1890.13873188778131743No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACA1850.13579576317218903No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT1850.13579576317218903No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATAC1820.13359366971534273No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.13285963856306063No Hit
GGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTTATACAC1810.13285963856306063No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1770.1299235139539322No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT1760.1291894828016501No Hit
GTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATAC1750.128455451649368No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCCTGTCTCTTATACAC1720.1262533581925217No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT1690.12405126473567538No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.12111514012654698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.11964707782198276No Hit
GCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTC1570.11524289090829015No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGT1570.11524289090829015No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1550.11377482860372595No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.11230676629916173No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCCTGTCTC1490.10937064169003333No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1470.10790257938546911No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.1034983924717765No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1400.1027643613194944No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCT1400.1027643613194944No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT1390.1020303301672123No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT1380.1012962990149302No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA200.001836245637.010
CGTTATA200.001836245637.02
AAATTCC200.001836245637.015
TCCTAAG200.001836245637.02
CCTAAGG200.001836245637.03
GCGAAAT200.001836245637.012
CTAAGGT200.001836245637.04
TTCCTTG200.001836245637.018
CTGTGGT200.001836245637.025
AAGGTAG200.001836245637.06
GCGTTAT200.001836245637.01
TGTGGTA200.001836245637.026
AGGTAGC200.001836245637.07
AGCTGTG200.001836245637.023
GTTATAC200.001836245637.03
TAAGGTA200.001836245637.05
TATTGAC1650.033.63636430
ATTGACT1650.033.63636431
GAAATTC250.005479558429.614
TAAGTTC250.005479558429.630
Produced by FastQC (version 0.11.5)