FastQCFastQC Report
Fri 10 Feb 2017
ERR1631443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21082
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2881.366094298453657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2761.3091737026847547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1980.9391898301868894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.5597191917275401No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA820.38895740442083293No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.25139929797931887No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA490.23242576605635137No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT370.175505170287449No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA370.175505170287449No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.16601840432596526No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.16127502134522342No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC320.1517882553837397No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC320.1517882553837397No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1375581064415141No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT270.12807134048003038No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA260.1233279574992885No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1233279574992885No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT250.11858457451854663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGC500.006813531318.521
CGCCTTA500.006813531318.525
CTCTTAT751.9485407E-417.26666637
ATGGGCA1201.7259063E-615.4166665
GGTATCA1651.3624231E-814.5757571
CTCTATG1000.001730871512.9500011
TCTATGG1153.324637E-412.8695652
TATGGGC1306.386633E-512.8076934
CTATGGG1256.7154283E-411.843
TCTCTTA1100.003540326111.77272736
GTATCAA2103.2023308E-711.4523822
AACGCAG2502.1367669E-811.0999995
TCAACGC2603.8113285E-810.6730773
TATCAAC2603.8113285E-810.6730771
ATCAACG2603.8113285E-810.6730772
CAACGCA2655.0446033E-810.4716984
AGAGTAC3401.9566505E-78.7058825
CGCAGAG3207.881408E-78.6718752
GAGTACA2000.00339654798.3250016
ACGCAGA3351.5244259E-68.2835821
Produced by FastQC (version 0.11.5)