##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631443.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21082
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87496442462764	34.0	33.0	34.0	31.0	34.0
2	32.97486007020207	34.0	33.0	34.0	31.0	34.0
3	33.042500711507444	34.0	33.0	34.0	31.0	34.0
4	36.34674129589223	37.0	37.0	37.0	35.0	37.0
5	36.33355469120577	37.0	37.0	37.0	35.0	37.0
6	36.40646048761977	37.0	37.0	37.0	35.0	37.0
7	36.41404990038896	37.0	37.0	37.0	35.0	37.0
8	36.45579167061948	37.0	37.0	37.0	35.0	37.0
9	38.255099136704295	39.0	39.0	39.0	37.0	39.0
10	38.2282515890333	39.0	39.0	39.0	37.0	39.0
11	38.29978180438289	39.0	39.0	39.0	37.0	39.0
12	38.23489232520634	39.0	39.0	39.0	37.0	39.0
13	38.28702210416469	39.0	39.0	39.0	37.0	39.0
14	39.729627170097714	41.0	40.0	41.0	38.0	41.0
15	39.67915757518262	41.0	40.0	41.0	37.0	41.0
16	39.59875723365904	41.0	39.0	41.0	37.0	41.0
17	39.648230718148184	41.0	39.0	41.0	37.0	41.0
18	39.67920500901243	41.0	40.0	41.0	37.0	41.0
19	39.682667678588366	41.0	40.0	41.0	37.0	41.0
20	39.663409543686555	41.0	40.0	41.0	37.0	41.0
21	39.639408025804	41.0	40.0	41.0	37.0	41.0
22	39.53695095341998	41.0	40.0	41.0	37.0	41.0
23	39.461958068494454	41.0	40.0	41.0	36.0	41.0
24	39.41072953230244	41.0	39.0	41.0	36.0	41.0
25	39.38449862441894	41.0	39.0	41.0	36.0	41.0
26	39.184659899440284	41.0	39.0	41.0	35.0	41.0
27	39.02594630490466	41.0	39.0	41.0	35.0	41.0
28	38.897732662935205	41.0	38.0	41.0	35.0	41.0
29	38.76197704202637	40.0	38.0	41.0	35.0	41.0
30	38.56280239066502	40.0	38.0	41.0	35.0	41.0
31	38.38758182335642	40.0	37.0	41.0	35.0	41.0
32	38.218907124561234	40.0	37.0	41.0	35.0	41.0
33	38.04197893937957	40.0	36.0	41.0	35.0	41.0
34	37.82957024950194	40.0	35.0	41.0	34.0	41.0
35	37.68717389242008	40.0	35.0	41.0	34.0	41.0
36	37.43895266103785	40.0	35.0	41.0	33.0	41.0
37	37.224504316478516	40.0	35.0	41.0	33.0	41.0
38	36.95934920785504	39.0	35.0	41.0	33.0	41.0
39	36.71553932264491	39.0	35.0	41.0	33.0	41.0
40	36.43567972678114	39.0	35.0	41.0	32.0	41.0
41	36.11962811877431	39.0	35.0	41.0	31.0	41.0
42	35.76178730670714	39.0	35.0	41.0	29.0	41.0
43	34.804382885874205	37.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.0
21	7.0
22	10.0
23	18.0
24	37.0
25	38.0
26	64.0
27	89.0
28	141.0
29	172.0
30	243.0
31	287.0
32	338.0
33	418.0
34	562.0
35	812.0
36	1287.0
37	3026.0
38	4380.0
39	9149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.95389431742719	16.388388198463144	13.347879707807609	24.30983777630206
2	20.979982923821268	19.974385731903993	31.391708566549664	27.653922777725075
3	22.255952945640832	21.781614647566645	27.929038990608102	28.03339341618442
4	15.933023432311924	16.687221326249883	29.347310501849922	38.03244473958827
5	16.260316857983113	35.40935395123802	29.79318850203965	18.53714068873921
6	32.881130822502605	29.94497675742339	17.616924390475287	19.55696802959871
7	30.865193055687318	30.29124371501755	18.148183284318375	20.695379944976757
8	32.66767858836923	28.891945735698698	17.820889858647188	20.61948581728489
9	28.996300161275023	12.204724409448819	18.56560098662366	40.2333744426525
10	22.085191158334126	25.064035670240013	25.362868798026756	27.487904373399108
11	35.6560098662366	22.127881605160802	17.408215539322644	24.807892989279953
12	23.489232520633717	27.212788160516084	24.466369414666538	24.83160990418366
13	34.77848401479935	17.27065743288113	24.219713499667964	23.731145052651552
14	24.855326819087374	19.922208519115834	23.982544350630867	31.239920311165925
15	30.988521013186602	22.711317711792052	20.628972583246373	25.671188691774976
16	26.17873067071435	23.503462669575942	24.660848116876956	25.656958542832747
17	23.228346456692915	27.117920500901242	24.56123707428138	25.092495968124467
18	24.05843847832274	21.914429371027417	27.473674224456886	26.55345792619296
19	24.59444075514657	25.42927615975714	27.70135660753249	22.2749264775638
20	26.131296840906938	21.985580115738546	28.934636182525374	22.948486860829142
21	27.82468456503178	21.501755051702876	26.743193245422635	23.93036713784271
22	27.174841096670143	22.014040413622997	25.20159377668153	25.609524713025326
23	25.173133478797077	22.943743477848404	26.482307181481836	25.40081586187269
24	24.41893558485912	23.631534010055972	27.59700218195617	24.352528223128736
25	24.56123707428138	23.08604496727066	26.07437624513803	26.27834171330993
26	25.642728393890522	23.147708946020302	26.107579926003226	25.101982734085947
27	24.380988521013187	23.384878095057395	25.728109287543877	26.506024096385545
28	24.09164215918793	23.076558201309176	27.151124181766434	25.680675457736456
29	23.256806754577365	23.901906839958258	28.355943458874872	24.48534294658951
30	23.07181481832843	23.906650222939	29.280903140119534	23.740631818613032
31	23.935110520823454	24.8695569680296	26.719476330518926	24.475856180628025
32	22.981690541694334	24.83160990418366	28.820794990987576	23.365904563134425
33	24.148562754956835	23.508206052556684	28.0191632672422	24.324067925244282
34	24.23868703159093	23.797552414381936	25.9795085855232	25.984251968503933
35	23.32321411630775	24.33829807418651	27.52585143724504	24.812636372260695
36	22.51683900958163	24.001517882553838	28.07134048003036	25.41030262783417
37	22.995920690636563	24.224456882648703	27.781994118205105	24.99762830850963
38	22.417227966986054	22.858362584195046	29.45166492742624	25.27274452139266
39	23.45602883976852	22.569016222369793	28.986813395313536	24.988141542548146
40	22.213262498814153	22.45043164785125	29.048477374063182	26.28782847927142
41	20.524618157670048	22.668627264965373	30.53789963001613	26.268854947348448
42	21.041646902570914	22.08044777535338	30.253296651171617	26.624608670904088
43	20.65268949815008	23.512949435537426	29.940233374442656	25.89412769186984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	19.0
4	36.0
5	36.0
6	52.0
7	68.0
8	72.0
9	76.0
10	112.5
11	149.0
12	149.0
13	268.0
14	387.0
15	507.5
16	628.0
17	559.5
18	491.0
19	491.0
20	543.5
21	596.0
22	406.5
23	217.0
24	194.0
25	171.0
26	171.0
27	186.5
28	202.0
29	210.5
30	219.0
31	238.0
32	257.0
33	257.0
34	263.0
35	269.0
36	287.0
37	305.0
38	358.5
39	412.0
40	412.0
41	501.5
42	591.0
43	683.0
44	775.0
45	959.5
46	1144.0
47	1144.0
48	1385.5
49	1627.0
50	1626.0
51	1625.0
52	1440.5
53	1256.0
54	1256.0
55	1165.0
56	1074.0
57	1085.0
58	1096.0
59	1130.5
60	1165.0
61	1165.0
62	1217.0
63	1269.0
64	1223.0
65	1177.0
66	1122.0
67	1067.0
68	1067.0
69	1011.5
70	956.0
71	839.0
72	722.0
73	597.5
74	473.0
75	473.0
76	387.5
77	302.0
78	225.5
79	149.0
80	113.5
81	78.0
82	78.0
83	53.5
84	29.0
85	21.5
86	14.0
87	9.0
88	4.0
89	4.0
90	2.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	21082.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.38781899250546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.9605043468248	77.41201024570724
2	3.690483044504577	6.081016981311071
3	0.8866371120962635	2.1914429371027415
4	0.4951350106511601	1.6317237453752016
5	0.2015084345673326	0.8300920216298264
6	0.16120674765386608	0.7968883407646333
7	0.10939029305083771	0.6308699364386681
8	0.09787552536127583	0.6451000853808937
9	0.04030168691346652	0.2988331277867375
>10	0.32241349530773217	4.667488853049996
>50	0.011514767689561863	0.6403567024001517
>100	0.023029535379123726	4.174177023052842
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	1.366094298453657	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	276	1.3091737026847547	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.9391898301868894	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.5597191917275401	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	82	0.38895740442083293	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.25139929797931887	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	49	0.23242576605635137	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	37	0.175505170287449	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	37	0.175505170287449	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.16601840432596526	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.16127502134522342	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	32	0.1517882553837397	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	32	0.1517882553837397	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29	0.1375581064415141	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	27	0.12807134048003038	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	26	0.1233279574992885	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26	0.1233279574992885	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	25	0.11858457451854663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.004743382980741865	0.0
6	0.0	0.0	0.0	0.004743382980741865	0.0
7	0.0	0.0	0.0	0.004743382980741865	0.0
8	0.0	0.0	0.0	0.004743382980741865	0.0
9	0.0	0.0	0.0	0.004743382980741865	0.0
10	0.0	0.0	0.0	0.014230148942225596	0.0
11	0.0	0.0	0.0	0.01897353192296746	0.0
12	0.0	0.0	0.0	0.01897353192296746	0.0
13	0.0	0.0	0.0	0.01897353192296746	0.0
14	0.0	0.0	0.0	0.033203680865193055	0.0
15	0.0	0.0	0.0	0.04743382980741865	0.0
16	0.0	0.0	0.0	0.056920595768902384	0.0
17	0.0	0.0	0.0	0.056920595768902384	0.0
18	0.0	0.0	0.0	0.056920595768902384	0.0
19	0.0	0.0	0.0	0.061663978749644244	0.0
20	0.0	0.0	0.0	0.06640736173038611	0.0
21	0.0	0.0	0.0	0.08063751067261171	0.0
22	0.0	0.0	0.0	0.09961104259557917	0.0
23	0.0	0.0	0.0	0.11858457451854663	0.0
24	0.0	0.0	0.0	0.1375581064415141	0.0
25	0.0	0.0	0.0	0.14704487240299782	0.0
26	0.0	0.0	0.0	0.16127502134522342	0.0
27	0.0	0.0	0.0	0.2608860639408026	0.0
28	0.0	0.0	0.0	0.4648515321127028	0.0
29	0.0	0.0	0.0	0.7447111279764729	0.0
30	0.0	0.0	0.0	1.157385447301015	0.0
31	0.0	0.0	0.0	1.826202447585618	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCGC	50	0.0068135313	18.5	21
CGCCTTA	50	0.0068135313	18.5	25
CTCTTAT	75	1.9485407E-4	17.266666	37
ATGGGCA	120	1.7259063E-6	15.416666	5
GGTATCA	165	1.3624231E-8	14.575757	1
CTCTATG	100	0.0017308715	12.950001	1
TCTATGG	115	3.324637E-4	12.869565	2
TATGGGC	130	6.386633E-5	12.807693	4
CTATGGG	125	6.7154283E-4	11.84	3
TCTCTTA	110	0.0035403261	11.772727	36
GTATCAA	210	3.2023308E-7	11.452382	2
AACGCAG	250	2.1367669E-8	11.099999	5
TCAACGC	260	3.8113285E-8	10.673077	3
TATCAAC	260	3.8113285E-8	10.673077	1
ATCAACG	260	3.8113285E-8	10.673077	2
CAACGCA	265	5.0446033E-8	10.471698	4
AGAGTAC	340	1.9566505E-7	8.705882	5
CGCAGAG	320	7.881408E-7	8.671875	2
GAGTACA	200	0.0033965479	8.325001	6
ACGCAGA	335	1.5244259E-6	8.283582	1
>>END_MODULE