FastQCFastQC Report
Fri 10 Feb 2017
ERR1631442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56341
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27794.932464812481141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22103.922543085852221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18133.2179052554977723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8041.4270247244457854No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4140.7348112387071581No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.5786194778225449No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2910.5164977547434373No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2590.4597007507853961No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.44727640616957454No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.4277524360589978No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2150.38160487034308943No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.34965655561669123No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1870.33190749187980334No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1820.3230329600113594No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.31948314726398186No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.30705880264816027No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1700.30173408352709397No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1630.28930973891127243No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1600.2839850197902061No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1600.2839850197902061No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1590.2822101134165173No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.2786603006691397No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1550.2751104879217621No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1540.27333558154807336No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1520.26978576880069577No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.2218632967110985No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.2200883903374097No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1210.21476367121634332No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT1130.20056442022683305No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1120.19878951385314422No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1090.19346479473207787No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1060.1881400756110115No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA970.17216591824781244No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.1650662927530573No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT920.1632913863793685No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG910.1615164800056797No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA880.15619176088461334No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA870.15441685451092454No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.15264194813723575No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT850.15086704176354698No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA850.15086704176354698No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT840.1490921353898582No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG840.1490921353898582No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT830.1473172290161694No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.14199250989510304No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT770.13666779077403668No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG770.13666779077403668No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT750.1331179780266591No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC750.1331179780266591No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC690.12246853978452636No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT640.11359400791608242No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11359400791608242No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT630.11181910154239363No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA630.11181910154239363No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT610.10826928879501606No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA610.10826928879501606ABI Solid3 Adapter B (95% over 23bp)
GTACATGGGCCTCTCTATGGGCATATCAATAAGCGGAGGAATC600.10649438242132728No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA600.10649438242132728No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA600.10649438242132728No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT590.1047194760476385No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT580.1029445696739497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC580.1029445696739497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC580.1029445696739497No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT580.1029445696739497No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT570.10116966330026092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAC200.001827109537.030
ATTGACT200.001827109537.031
TCACTGT200.001827109537.035
TGACTGT251.2187757E-436.99999633
GACTGTC251.2187757E-436.99999634
AGGGCAA303.556555E-430.83333410
TTGACTG303.556555E-430.83333432
TATCACT250.005452607329.59999833
ATCACTG250.005452607329.59999834
CAGCGTC250.005452607329.5999982
AGCGTCA250.005452607329.5999983
GAGGGCA250.005452607329.5999989
ATGCCCC405.841913E-527.75000237
GGGCAAA358.764624E-426.42857211
ACCGACC358.764624E-426.42857232
TCCTCCG802.6111593E-823.12500221
CCGCTTA802.6111593E-823.12500225
AGAGGGC400.001908590423.1250028
TCCGCTT802.6111593E-823.12500224
CTCCGCT802.6111593E-823.12500223
Produced by FastQC (version 0.11.5)