FastQCFastQC Report
Fri 10 Feb 2017
ERR1631441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513287
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.26008841057731835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10980.21391541184561463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9180.17884731154305486No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6540.12741409776596718No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5990.11669884489574059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG250.005493544529.65
CATAATA400.001929739123.1252
GGTATCA6000.021.8916651
GTTCTAG1201.0913936E-1121.5833321
ATACTGA450.003822965820.5555576
ACCTTAC450.003822965820.55555722
GTGTAAG555.1377655E-420.1818181
CGCAGTA555.1377655E-420.1818182
CTAGATG1303.274181E-1119.9230774
ACTGATC656.8926216E-519.9230778
TGTCCGA1303.274181E-1119.92307712
ATACACA2050.019.8536637
CTAAGGA500.007029707518.54
CGGACCA2250.017.2666669
GAGCGAA2150.017.20930316
TCTGCAA650.001578323817.07692310
TATTTAA650.001578323817.0769232
GGATTAT650.001578323817.0769231
CCGCAGT650.001578323817.0769231
CGAACGA1107.786912E-716.81818216
Produced by FastQC (version 0.11.5)