##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631441.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513287
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.815387882412764	34.0	31.0	34.0	31.0	34.0
2	32.98481746079678	34.0	33.0	34.0	31.0	34.0
3	33.097709468581904	34.0	33.0	34.0	31.0	34.0
4	36.432333178124516	37.0	37.0	37.0	35.0	37.0
5	36.41018962101125	37.0	37.0	37.0	35.0	37.0
6	36.45257916136586	37.0	37.0	37.0	35.0	37.0
7	36.445247980174834	37.0	37.0	37.0	35.0	37.0
8	36.43486977071307	37.0	37.0	37.0	35.0	37.0
9	38.28873904852451	39.0	39.0	39.0	37.0	39.0
10	38.23939043848763	39.0	39.0	39.0	37.0	39.0
11	38.3036780592534	39.0	39.0	39.0	37.0	39.0
12	38.08191518585119	39.0	39.0	39.0	37.0	39.0
13	38.211684691020814	39.0	39.0	39.0	37.0	39.0
14	39.72890215415547	41.0	40.0	41.0	38.0	41.0
15	39.72900735845638	41.0	40.0	41.0	38.0	41.0
16	39.69758634058529	41.0	40.0	41.0	37.0	41.0
17	39.70068986746206	41.0	40.0	41.0	38.0	41.0
18	39.672019747236924	41.0	40.0	41.0	37.0	41.0
19	39.6801984075186	41.0	40.0	41.0	37.0	41.0
20	39.65030090378287	41.0	40.0	41.0	37.0	41.0
21	39.620483277386725	41.0	40.0	41.0	37.0	41.0
22	39.56784216237699	41.0	40.0	41.0	37.0	41.0
23	39.498181329353756	41.0	39.0	41.0	37.0	41.0
24	39.45104006140814	41.0	39.0	41.0	37.0	41.0
25	39.44001504031858	41.0	39.0	41.0	36.0	41.0
26	39.35744914638399	41.0	39.0	41.0	36.0	41.0
27	39.29154449654872	41.0	39.0	41.0	36.0	41.0
28	39.20109022827385	41.0	39.0	41.0	36.0	41.0
29	39.121497329953804	41.0	39.0	41.0	35.0	41.0
30	39.05901181989803	40.0	39.0	41.0	35.0	41.0
31	39.015698819568776	40.0	39.0	41.0	35.0	41.0
32	38.93015603356407	40.0	39.0	41.0	35.0	41.0
33	38.83083732882383	40.0	38.0	41.0	35.0	41.0
34	38.79053823689281	40.0	38.0	41.0	35.0	41.0
35	38.67722930835965	40.0	38.0	41.0	35.0	41.0
36	38.61618548687186	40.0	38.0	41.0	35.0	41.0
37	38.5517166809212	40.0	38.0	41.0	35.0	41.0
38	38.451934297965856	40.0	38.0	41.0	35.0	41.0
39	38.37028601932252	40.0	38.0	41.0	35.0	41.0
40	38.2783783731129	40.0	38.0	41.0	34.0	41.0
41	38.18758316497398	40.0	37.0	41.0	34.0	41.0
42	38.06644041247879	40.0	37.0	41.0	34.0	41.0
43	37.13006953224999	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	1.0
14	0.0
15	0.0
16	5.0
17	6.0
18	16.0
19	26.0
20	44.0
21	90.0
22	168.0
23	260.0
24	401.0
25	586.0
26	890.0
27	1151.0
28	1662.0
29	2389.0
30	3245.0
31	4300.0
32	5496.0
33	7570.0
34	11067.0
35	16467.0
36	26078.0
37	49081.0
38	109596.0
39	272688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30286954471865	17.89622569829355	12.165318817737445	26.63558593925036
2	19.7634072166254	20.259231190347702	32.67567657080737	27.30168502221954
3	20.08681303052678	21.30776738939424	29.116654035656463	29.488765544422517
4	14.869264173844263	15.5760812177203	33.139939254257364	36.41471535417807
5	16.378751069090004	34.55980767095212	32.365908351468086	16.695532908489792
6	34.87230340920382	33.6714157966206	15.803634224128022	15.652646570047557
7	30.540029262381474	29.440449495116766	20.162209446177286	19.857311796324474
8	28.7318790462256	30.951494972598177	19.5791048672575	20.737521113918724
9	27.60658267207235	13.944440439753977	19.03769236314187	39.4112845250318
10	19.033211439214316	24.71054205541929	30.47106199845311	25.78518450691329
11	37.544882297817786	20.93195424781847	19.9500474393468	21.57311601501694
12	23.5090699744977	24.83542345705229	26.61473990184828	25.040766666601726
13	31.27158879924876	17.841480497265664	24.128216767617335	26.75871393586824
14	24.134645919339473	19.980244970162893	23.034092038177473	32.85101707232017
15	27.27226678252128	25.911040022443583	20.508214702495874	26.308478492539262
16	26.8724904390721	24.601051653363516	22.50592748306503	26.020530424499356
17	25.775833013499273	24.98933345282464	23.27547746192676	25.959356071749333
18	25.489638350474493	23.044807291047697	25.23013440823555	26.23541995024226
19	26.347443048430996	23.95696754447317	24.432529949131773	25.263059457964065
20	26.704358380399267	22.831865993099377	24.137373438251895	26.32640218824946
21	27.900765069054934	23.60433831365299	23.221706374796167	25.273190242495915
22	27.586905571347025	23.069549784038948	23.009739190745137	26.333805453868887
23	26.003580842686453	23.186833097273066	24.05028765583387	26.759298404206618
24	25.606142372590774	23.951707329427784	24.142438830517822	26.29971146746362
25	26.184960850362465	22.813357829050805	23.914301355771723	27.087379964815007
26	25.728296255311356	24.262254839884896	24.19211863927978	25.817330265523964
27	26.91048088106654	23.04305388603257	23.366070054375037	26.680395178525856
28	25.38462887234627	23.66239550193167	24.66397941112867	26.288996214593396
29	25.069795260741067	23.792537118610056	24.950758542491823	26.186909078157054
30	24.654822740494108	24.502666149736893	25.052456033369246	25.790055076399753
31	26.009425526070213	24.06938028822082	23.685189767128332	26.236004418580638
32	24.731388092821366	23.63531513558691	24.450453644841968	27.182843126749752
33	24.4545449232106	23.28521860089969	25.24805810394575	27.01217837194396
34	25.807394303771574	22.63178299859533	25.28838641929369	26.272436278339406
35	25.72050334413301	22.82426790470049	25.80661501265374	25.64861373851276
36	24.211600917225645	23.40951553419432	25.754597330538274	26.62428621804176
37	24.89387029088989	22.017896420521073	26.259772797674596	26.82846049091444
38	24.123151375351412	21.695464720516984	27.591581318054033	26.589802586077578
39	24.181598209189012	21.26958212462034	27.655093544157555	26.89372612203309
40	23.371525092199878	21.826580451092664	28.418993662414984	26.382900794292475
41	22.289089729527536	21.627861216044824	29.808274902734727	26.274774151692913
42	23.042664240473655	21.658253569640376	29.334855548650168	25.9642266412358
43	21.597468862449272	21.008714422925188	29.76658282793057	27.627233886694967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	80.0
2	78.0
3	164.5
4	251.0
5	251.0
6	304.0
7	357.0
8	343.5
9	330.0
10	492.0
11	654.0
12	654.0
13	1035.5
14	1417.0
15	2106.0
16	2795.0
17	2576.5
18	2358.0
19	2358.0
20	2660.5
21	2963.0
22	2723.0
23	2483.0
24	2827.0
25	3171.0
26	3171.0
27	3698.5
28	4226.0
29	5347.0
30	6468.0
31	7847.0
32	9226.0
33	9226.0
34	10912.5
35	12599.0
36	15035.0
37	17471.0
38	19991.0
39	22511.0
40	22511.0
41	24427.5
42	26344.0
43	27336.0
44	28328.0
45	30364.5
46	32401.0
47	32401.0
48	33953.0
49	35505.0
50	37628.5
51	39752.0
52	41194.5
53	42637.0
54	42637.0
55	40651.0
56	38665.0
57	37452.0
58	36239.0
59	34350.0
60	32461.0
61	32461.0
62	30533.5
63	28606.0
64	25919.0
65	23232.0
66	20766.5
67	18301.0
68	18301.0
69	15857.5
70	13414.0
71	11789.5
72	10165.0
73	8097.5
74	6030.0
75	6030.0
76	4881.5
77	3733.0
78	3223.5
79	2714.0
80	2349.0
81	1984.0
82	1984.0
83	1662.0
84	1340.0
85	1121.5
86	903.0
87	813.5
88	724.0
89	724.0
90	505.5
91	287.0
92	171.5
93	56.0
94	37.5
95	19.0
96	19.0
97	11.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	513287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.42927542362274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.11850539985498	52.07129697349737
2	5.252310454312336	6.137773882907771
3	1.688180828209291	2.9591754772896066
4	0.8771394928968933	2.0500250006163747
5	0.5402258071337486	1.5782501237983346
6	0.3816822842913662	1.3380851587906628
7	0.29351658207434084	1.2004972850795423
8	0.21561327646036588	1.0078502012233947
9	0.1664156992166569	0.8751193851910326
>10	1.226018948525852	14.393953441979034
>50	0.14644138739391876	5.979952606671364
>100	0.0919437932325181	9.40937560754378
>500	0.0013373642652002634	0.5233508543912674
>1k	6.686821326001317E-4	0.4752940010205127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1335	0.26008841057731835	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.21391541184561463	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	918	0.17884731154305486	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	654	0.12741409776596718	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	599	0.11669884489574059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.9482277945866543E-4	0.0	0.0	0.0
6	0.0	1.9482277945866543E-4	0.0	0.0	0.0
7	0.0	1.9482277945866543E-4	0.0	0.0	0.0
8	0.0	1.9482277945866543E-4	0.0	0.0	0.0
9	0.0	1.9482277945866543E-4	0.0	0.0	0.0
10	0.0	1.9482277945866543E-4	0.0	0.0	0.0
11	0.0	1.9482277945866543E-4	0.0	0.0	0.0
12	0.0	1.9482277945866543E-4	0.0	0.0	0.0
13	0.0	1.9482277945866543E-4	0.0	0.0	0.0
14	0.0	1.9482277945866543E-4	0.0	1.9482277945866543E-4	0.0
15	0.0	1.9482277945866543E-4	0.0	5.844683383759963E-4	0.0
16	0.0	1.9482277945866543E-4	0.0	9.741138972933271E-4	0.0
17	0.0	1.9482277945866543E-4	0.0	0.0011689366767519925	0.0
18	0.0	3.8964555891733086E-4	0.0	0.001363759456210658	0.0
19	0.0	3.8964555891733086E-4	0.0	0.0015585822356693234	0.0
20	0.0	3.8964555891733086E-4	0.0	0.0015585822356693234	0.0
21	0.0	3.8964555891733086E-4	0.0	0.001753405015127989	0.0
22	0.0	3.8964555891733086E-4	0.0	0.003506810030255978	0.0
23	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.006039506163218628	0.0
24	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.010520430090767932	0.0
25	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.014611708459399906	0.0
26	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.021820151299370528	0.0
27	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.07169478284078888	0.0
28	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.24430776544116645	0.0
29	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.48881035366179154	0.0
30	1.9482277945866543E-4	3.8964555891733086E-4	0.0	0.8196194331826054	0.0
31	1.9482277945866543E-4	3.8964555891733086E-4	0.0	1.562868336817414	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	25	0.0054935445	29.6	5
CATAATA	40	0.0019297391	23.125	2
GGTATCA	600	0.0	21.891665	1
GTTCTAG	120	1.0913936E-11	21.583332	1
ATACTGA	45	0.0038229658	20.555557	6
ACCTTAC	45	0.0038229658	20.555557	22
GTGTAAG	55	5.1377655E-4	20.181818	1
CGCAGTA	55	5.1377655E-4	20.181818	2
CTAGATG	130	3.274181E-11	19.923077	4
ACTGATC	65	6.8926216E-5	19.923077	8
TGTCCGA	130	3.274181E-11	19.923077	12
ATACACA	205	0.0	19.85366	37
CTAAGGA	50	0.0070297075	18.5	4
CGGACCA	225	0.0	17.266666	9
GAGCGAA	215	0.0	17.209303	16
TCTGCAA	65	0.0015783238	17.076923	10
TATTTAA	65	0.0015783238	17.076923	2
GGATTAT	65	0.0015783238	17.076923	1
CCGCAGT	65	0.0015783238	17.076923	1
CGAACGA	110	7.786912E-7	16.818182	16
>>END_MODULE