FastQCFastQC Report
Fri 10 Feb 2017
ERR1631440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256554
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68892.68520467425961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55022.144577749713511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50981.987106028360501No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.5647933768329475No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA14180.5527101506895234No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT10270.4003055886869821No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC9770.38081651426210467No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT9480.3695128510956758No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC9260.3609376583487297No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8510.33170404671141357No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT8500.331314265222916No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT8330.3246879799184577No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA7710.3005215276316097No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA7030.2740163864137764No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA6780.2642718492013377No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA6700.26115359729335735No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT6230.24283386733397258No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA6090.23737692649500688No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.2245141373745878No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.20619440741520303No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT5080.19800899615675452No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4970.1937213997832815No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.19177249234079374No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT4840.18865424043281337No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.17384254386990655No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT4450.17345276238140897No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG4370.1703345104734286No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT4300.16760604005394575No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4070.15864106581850215No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT4040.15747172135300952No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC3920.15279434349103893No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC3850.15006587307155608No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA3590.13993155437061983No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG3560.13876220990512717No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA3550.13837242841662964No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA3360.13096658013517623No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT3260.12706876525020075No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT3090.12044247994574242No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC3080.12005269845724487No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA2960.11537532059527429No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.11498553910677674No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT2860.11147750571029881No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2780.10835925380231841No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT2760.10757969082532332No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA2690.10485122040584048ABI Solid3 Adapter B (100% over 21bp)
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.10407165742884539No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT2630.10251253147485519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATC600.037.01
TAATCCT200.001839272437.04
GCTCTAT750.032.0666661
AGTATCA751.4551915E-1129.62
GGAGTAC502.7068745E-729.5999981
ATTGACT2200.026.9090931
TATTGAC2350.025.97872430
ATATGCT509.0551985E-625.89999835
GATACAC451.319302E-424.6666663
ATGCCCC2350.023.61702237
TATCACT1103.6379788E-1223.54545433
TTATTGC551.8952E-523.54545429
TTGACTG2700.023.29629732
CTCCGAG400.001927122723.1259
GGGTTGA400.001927122723.1251
TCACTGT1155.456968E-1222.5217435
TATTGCT502.6941826E-422.19999930
GGGTATC1950.021.8205131
TGACTGT2900.021.68965533
ATCACTG1201.0913936E-1121.58333234
Produced by FastQC (version 0.11.5)