##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631440.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256554
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9658590394225	31.0	31.0	33.0	28.0	34.0
2	31.1104134022467	31.0	31.0	34.0	28.0	34.0
3	31.025791841093884	31.0	31.0	34.0	27.0	34.0
4	35.05963266992524	35.0	35.0	37.0	32.0	37.0
5	34.112732602103264	35.0	35.0	37.0	30.0	37.0
6	34.048079546606175	35.0	33.0	37.0	30.0	37.0
7	34.085787007803425	35.0	33.0	37.0	30.0	37.0
8	34.05325194695853	35.0	33.0	37.0	30.0	37.0
9	35.45357702471994	37.0	34.0	39.0	30.0	39.0
10	35.21315590479977	37.0	34.0	39.0	30.0	39.0
11	35.349505367291094	37.0	34.0	39.0	30.0	39.0
12	35.06411125922808	37.0	34.0	39.0	29.0	39.0
13	35.19495700710182	37.0	34.0	39.0	30.0	39.0
14	35.87505554386211	38.0	34.0	40.0	29.0	41.0
15	35.73617250169555	38.0	34.0	40.0	29.0	41.0
16	35.5972855617141	38.0	34.0	40.0	28.0	41.0
17	35.60896731292438	38.0	34.0	40.0	29.0	40.0
18	35.86199396618256	38.0	34.0	40.0	29.0	41.0
19	35.89063510995736	38.0	34.0	40.0	29.0	41.0
20	35.95654326184741	38.0	34.0	40.0	29.0	41.0
21	35.92833477552484	38.0	34.0	40.0	29.0	41.0
22	35.83030473116771	38.0	34.0	40.0	29.0	41.0
23	35.75208338205602	38.0	34.0	40.0	29.0	41.0
24	35.67735837289615	38.0	34.0	40.0	29.0	41.0
25	35.64425423107806	38.0	34.0	40.0	29.0	41.0
26	35.3974991619698	38.0	34.0	40.0	27.0	41.0
27	35.241239661046016	37.0	33.0	40.0	27.0	41.0
28	34.997591150401085	37.0	33.0	39.0	27.0	41.0
29	34.68052339858276	37.0	33.0	39.0	26.0	40.0
30	34.37567139861394	37.0	33.0	39.0	25.0	40.0
31	34.08953670572277	36.0	33.0	39.0	25.0	40.0
32	33.80445052503566	35.0	32.0	38.0	24.0	40.0
33	33.50049502249039	35.0	32.0	38.0	24.0	40.0
34	33.34736546691924	35.0	31.0	38.0	23.0	40.0
35	33.15335952664936	35.0	31.0	38.0	22.0	40.0
36	32.918231639342984	35.0	31.0	38.0	21.0	40.0
37	32.69781020759762	35.0	31.0	38.0	21.0	40.0
38	32.454036966876366	35.0	31.0	38.0	20.0	40.0
39	32.06663704327354	35.0	30.0	38.0	19.0	40.0
40	31.801285499349063	35.0	30.0	38.0	18.0	40.0
41	31.342578950240494	35.0	29.0	38.0	15.0	40.0
42	31.01113215931149	34.0	28.0	38.0	14.0	40.0
43	29.946592140446064	33.0	26.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	5.0
14	7.0
15	10.0
16	19.0
17	44.0
18	87.0
19	178.0
20	281.0
21	485.0
22	793.0
23	1264.0
24	1843.0
25	2720.0
26	3900.0
27	5442.0
28	7473.0
29	9683.0
30	12328.0
31	15427.0
32	19153.0
33	23334.0
34	27920.0
35	32319.0
36	34557.0
37	30823.0
38	19799.0
39	6654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.60734192411734	13.383537189051816	16.239466155273355	23.769654731557488
2	21.504244720409737	21.46955416793346	32.07862672185973	24.94757438979708
3	20.47872962417269	22.866920804197164	30.018631555150183	26.63571801647996
4	13.999391940877945	19.462179502171082	30.660211885217148	35.878216671733824
5	13.928451709971391	38.639038954761965	29.185278732742425	18.247230602524226
6	26.894143143353837	31.990925886947778	22.06397093789222	19.05096003180617
7	30.02915565533962	32.08954060353766	16.89118080404125	20.990122937081473
8	35.7554355028571	28.477825331119373	16.159560950131358	19.60717821589217
9	32.320681026216704	13.286091816927431	16.102652852810714	38.29057430404515
10	21.524123576323113	26.860621935343048	21.45162421946257	30.163630268871273
11	33.98037060423926	26.813458375234845	14.606281718468628	24.599889302057267
12	26.162913070932436	29.9999220437023	22.646304481707556	21.19086040365771
13	36.24110323752504	18.633893839113792	24.102528122734395	21.02247480062677
14	25.620727020432344	20.903591446635016	24.87117721805156	28.60450431488108
15	32.970446767542114	24.01404772484545	20.354779110830467	22.660726396781964
16	24.44631539558923	25.16234398995923	25.57824083818611	24.813099776265428
17	18.950006626285305	29.928202249818753	27.05239442768384	24.069396696212102
18	23.06337067439993	21.986404421681204	29.726295438777022	25.223929465141843
19	21.816459692696274	28.511736320618663	31.480312137015986	18.191491849669074
20	25.03059784684706	21.744739898812725	32.795045097718216	20.429617156621998
21	28.150408880781434	19.891718702495382	30.90967203785558	21.048200378867605
22	26.617398286520576	21.10510847618825	27.31120933604621	24.966283901244964
23	22.61044458476578	24.029249202896857	27.811688767277065	25.5486174450603
24	21.476180453237916	23.57125595391224	32.84727581717689	22.10528777567296
25	20.73442628062708	23.182254028391682	29.62300334432517	26.460316346656064
26	24.80569392798397	21.938851080084504	28.78107532917047	24.474379662761056
27	22.690349789907778	21.985235077215712	28.167559266275326	27.15685586660118
28	20.87591696095169	22.844703259352805	31.262424284945862	25.01695549474964
29	20.68804228349587	23.53578583845896	33.47755248407743	22.29861939396774
30	20.060104305526323	25.016565713261148	35.01835870810823	19.904971273104298
31	20.758982514402426	26.479415639592446	30.527686179127983	22.23391566687715
32	19.695268832292616	26.05065600224514	34.47227484272317	19.781800322739073
33	21.80554581101834	23.418071828932703	34.39977548586263	20.376606874186333
34	22.2576923376755	25.458188139728865	28.57916851812874	23.704951004466896
35	20.84902203824536	26.569844944923876	29.485410478885537	23.09572253794523
36	17.71868690412155	26.182012363868818	31.585553138910328	24.513747593099307
37	19.115663758896762	26.36248119304318	31.93323822665014	22.588616821409918
38	19.86170552788107	22.302906990341214	33.616704475471046	24.218683006306666
39	20.294752761601845	22.20663096268232	34.75798467379187	22.740631601923962
40	19.844555142387176	21.524123576323113	31.96052293084497	26.67079835044474
41	16.11785433086212	23.19394747304661	35.67981789408857	25.0083803020027
42	16.76021422390608	21.916633535240145	34.158500744482645	27.164651496371135
43	15.198749580984899	24.601838209499753	36.49874880142192	23.700663408093423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	8.0
2	13.0
3	98.0
4	183.0
5	183.0
6	349.0
7	515.0
8	653.5
9	792.0
10	1171.5
11	1551.0
12	1551.0
13	3021.0
14	4491.0
15	8475.0
16	12459.0
17	11453.5
18	10448.0
19	10448.0
20	11206.0
21	11964.0
22	8482.5
23	5001.0
24	4014.0
25	3027.0
26	3027.0
27	2690.5
28	2354.0
29	2356.5
30	2359.0
31	2386.0
32	2413.0
33	2413.0
34	2329.5
35	2246.0
36	2160.0
37	2074.0
38	2479.0
39	2884.0
40	2884.0
41	3875.5
42	4867.0
43	6838.0
44	8809.0
45	12060.0
46	15311.0
47	15311.0
48	22837.5
49	30364.0
50	29477.0
51	28590.0
52	23351.5
53	18113.0
54	18113.0
55	14509.5
56	10906.0
57	10750.5
58	10595.0
59	10406.0
60	10217.0
61	10217.0
62	10325.0
63	10433.0
64	10176.0
65	9919.0
66	9682.0
67	9445.0
68	9445.0
69	8766.0
70	8087.0
71	7140.0
72	6193.0
73	5314.0
74	4435.0
75	4435.0
76	3611.5
77	2788.0
78	2131.0
79	1474.0
80	1099.0
81	724.0
82	724.0
83	537.5
84	351.0
85	232.0
86	113.0
87	69.5
88	26.0
89	26.0
90	20.0
91	14.0
92	8.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	256554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.46541634343674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.91326195878744	40.77908748296592
2	7.717506599770256	7.326293277826321
3	2.0570940145426397	2.929224715735743
4	1.039811432406571	1.9742033023137289
5	0.6325608817763311	1.501238280804251
6	0.4443851308679308	1.2655755152087373
7	0.2943425182538023	0.9779763123544647
8	0.2140044530235893	0.8126248369691293
9	0.17138504449895114	0.7321376242963034
>10	1.2432282232165657	11.098632565429531
>50	0.1406676490383366	4.638528924319518
>100	0.11094913163587111	10.713520683286387
>500	0.014859258701232739	5.196657037159585
>1k	0.0029718517402465474	1.8309611384997668
>5k	0.0029718517402465474	8.223338302830618
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6889	2.68520467425961	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5502	2.144577749713511	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5098	1.987106028360501	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1449	0.5647933768329475	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1418	0.5527101506895234	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	1027	0.4003055886869821	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	977	0.38081651426210467	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	948	0.3695128510956758	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	926	0.3609376583487297	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	851	0.33170404671141357	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	850	0.331314265222916	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	833	0.3246879799184577	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	771	0.3005215276316097	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	703	0.2740163864137764	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	678	0.2642718492013377	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	670	0.26115359729335735	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	623	0.24283386733397258	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	609	0.23737692649500688	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	576	0.2245141373745878	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	529	0.20619440741520303	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	508	0.19800899615675452	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	497	0.1937213997832815	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	492	0.19177249234079374	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	484	0.18865424043281337	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	446	0.17384254386990655	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	445	0.17345276238140897	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	437	0.1703345104734286	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	430	0.16760604005394575	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	407	0.15864106581850215	No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	404	0.15747172135300952	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	392	0.15279434349103893	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	385	0.15006587307155608	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	359	0.13993155437061983	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	356	0.13876220990512717	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	355	0.13837242841662964	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	336	0.13096658013517623	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	326	0.12706876525020075	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	309	0.12044247994574242	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	308	0.12005269845724487	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	296	0.11537532059527429	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	295	0.11498553910677674	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	286	0.11147750571029881	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	278	0.10835925380231841	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	276	0.10757969082532332	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	269	0.10485122040584048	ABI Solid3 Adapter B (100% over 21bp)
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	267	0.10407165742884539	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	263	0.10251253147485519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	3.8978148849754825E-4	0.0	0.0
10	0.0	0.0	3.8978148849754825E-4	0.0	3.8978148849754825E-4
11	0.0	0.0	3.8978148849754825E-4	3.8978148849754825E-4	3.8978148849754825E-4
12	0.0	0.0	3.8978148849754825E-4	3.8978148849754825E-4	3.8978148849754825E-4
13	0.0	0.0	3.8978148849754825E-4	3.8978148849754825E-4	3.8978148849754825E-4
14	0.0	0.0	3.8978148849754825E-4	7.795629769950965E-4	3.8978148849754825E-4
15	0.0	0.0	3.8978148849754825E-4	0.0019489074424877413	3.8978148849754825E-4
16	0.0	0.0	7.795629769950965E-4	0.003118251907980386	3.8978148849754825E-4
17	0.0	0.0	7.795629769950965E-4	0.004677377861970579	3.8978148849754825E-4
18	0.0	0.0	7.795629769950965E-4	0.0058467223274632244	3.8978148849754825E-4
19	0.0	0.0	7.795629769950965E-4	0.0074058482814534175	7.795629769950965E-4
20	0.0	0.0	7.795629769950965E-4	0.008575192746946063	7.795629769950965E-4
21	0.0	0.0	7.795629769950965E-4	0.011693444654926449	7.795629769950965E-4
22	0.0	0.0	7.795629769950965E-4	0.019878855913374963	7.795629769950965E-4
23	0.0	0.0	7.795629769950965E-4	0.030792737591306314	7.795629769950965E-4
24	0.0	0.0	7.795629769950965E-4	0.04638399713120824	7.795629769950965E-4
25	0.0	0.0	7.795629769950965E-4	0.053789845412661665	7.795629769950965E-4
26	0.0	0.0	7.795629769950965E-4	0.06509350857909056	7.795629769950965E-4
27	0.0	0.0	7.795629769950965E-4	0.10290231296335274	7.795629769950965E-4
28	0.0	0.0	7.795629769950965E-4	0.23698714500650936	7.795629769950965E-4
29	0.0	7.795629769950965E-4	7.795629769950965E-4	0.516070690770754	7.795629769950965E-4
30	0.0	7.795629769950965E-4	7.795629769950965E-4	0.8692127193495327	7.795629769950965E-4
31	0.0	7.795629769950965E-4	7.795629769950965E-4	1.7777933690373178	7.795629769950965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTATC	60	0.0	37.0	1
TAATCCT	20	0.0018392724	37.0	4
GCTCTAT	75	0.0	32.066666	1
AGTATCA	75	1.4551915E-11	29.6	2
GGAGTAC	50	2.7068745E-7	29.599998	1
ATTGACT	220	0.0	26.90909	31
TATTGAC	235	0.0	25.978724	30
ATATGCT	50	9.0551985E-6	25.899998	35
GATACAC	45	1.319302E-4	24.666666	3
ATGCCCC	235	0.0	23.617022	37
TATCACT	110	3.6379788E-12	23.545454	33
TTATTGC	55	1.8952E-5	23.545454	29
TTGACTG	270	0.0	23.296297	32
CTCCGAG	40	0.0019271227	23.125	9
GGGTTGA	40	0.0019271227	23.125	1
TCACTGT	115	5.456968E-12	22.52174	35
TATTGCT	50	2.6941826E-4	22.199999	30
GGGTATC	195	0.0	21.820513	1
TGACTGT	290	0.0	21.689655	33
ATCACTG	120	1.0913936E-11	21.583332	34
>>END_MODULE