FastQCFastQC Report
Fri 10 Feb 2017
ERR1631439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034967
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.19459557647731762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16610.16048820880279274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14310.13826527802335728No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT12260.11845788319820826No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA11690.11295046122243513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAA450.003825554920.55555529
TAAGCTA450.003825554920.55555533
CTAAACA555.142619E-420.1818184
CAGTACT951.6757076E-719.4736844
GGTATCA8300.019.3915671
GTCTAGA3300.018.51
AGTGCGC902.1517953E-618.529
GCCTAAA500.007034419618.51
TAGGCCG801.6164166E-518.55
TCTAGAC3550.018.2394373
GTGTAAG1451.546141E-1017.862071
AAGACGG2800.017.8392855
CTAGACA3750.017.7599984
CGAGCCG3150.017.61904715
GTGCGCT953.6056845E-617.52631630
GTTCTAG3750.017.2666661
TACTATA752.0670157E-417.2666662
ACATGTA2600.017.0769258
GCACTAT650.001579789717.07692513
AACTATG1851.8189894E-1217.0000027
Produced by FastQC (version 0.11.5)