##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631439.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1034967
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.891811043250655	34.0	33.0	34.0	31.0	34.0
2	33.05483073373354	34.0	33.0	34.0	31.0	34.0
3	33.17147406632289	34.0	33.0	34.0	31.0	34.0
4	36.47677945287145	37.0	37.0	37.0	35.0	37.0
5	36.44962882874526	37.0	37.0	37.0	35.0	37.0
6	36.4909538178512	37.0	37.0	37.0	35.0	37.0
7	36.49055573752593	37.0	37.0	37.0	35.0	37.0
8	36.48338739302799	37.0	37.0	37.0	35.0	37.0
9	38.343585834137706	39.0	39.0	39.0	37.0	39.0
10	38.30812383390002	39.0	39.0	39.0	37.0	39.0
11	38.361301374826446	39.0	39.0	39.0	37.0	39.0
12	38.1507284773331	39.0	39.0	39.0	37.0	39.0
13	38.2741691280978	39.0	39.0	39.0	37.0	39.0
14	39.823172139788035	41.0	40.0	41.0	38.0	41.0
15	39.81676227357974	41.0	40.0	41.0	38.0	41.0
16	39.808606457983686	41.0	40.0	41.0	38.0	41.0
17	39.80655228620816	41.0	40.0	41.0	38.0	41.0
18	39.79276440698109	41.0	40.0	41.0	38.0	41.0
19	39.79365332421227	41.0	40.0	41.0	38.0	41.0
20	39.74950602289735	41.0	40.0	41.0	38.0	41.0
21	39.720027788325616	41.0	40.0	41.0	38.0	41.0
22	39.674436962724414	41.0	40.0	41.0	37.0	41.0
23	39.62716492409903	41.0	40.0	41.0	37.0	41.0
24	39.59291165805286	41.0	40.0	41.0	37.0	41.0
25	39.595350383152315	41.0	40.0	41.0	37.0	41.0
26	39.53072610044571	41.0	40.0	41.0	37.0	41.0
27	39.467435193585885	41.0	39.0	41.0	37.0	41.0
28	39.41439099024414	41.0	39.0	41.0	36.0	41.0
29	39.370041750123434	41.0	39.0	41.0	36.0	41.0
30	39.33363382600605	41.0	39.0	41.0	36.0	41.0
31	39.291128122925656	41.0	39.0	41.0	36.0	41.0
32	39.22143218092944	41.0	39.0	41.0	35.0	41.0
33	39.14901151437679	41.0	39.0	41.0	35.0	41.0
34	39.11536792960548	41.0	39.0	41.0	35.0	41.0
35	39.048139699140165	41.0	39.0	41.0	35.0	41.0
36	39.000590356987225	40.0	39.0	41.0	35.0	41.0
37	38.95084964061656	40.0	39.0	41.0	35.0	41.0
38	38.87087897488519	40.0	39.0	41.0	35.0	41.0
39	38.81106160872762	40.0	38.0	41.0	35.0	41.0
40	38.740132777180335	40.0	38.0	41.0	35.0	41.0
41	38.67158469786959	40.0	38.0	41.0	35.0	41.0
42	38.566429654278835	40.0	38.0	41.0	35.0	41.0
43	37.67981297954427	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	0.0
14	0.0
15	2.0
16	3.0
17	6.0
18	16.0
19	29.0
20	48.0
21	124.0
22	183.0
23	323.0
24	549.0
25	834.0
26	1276.0
27	1828.0
28	2637.0
29	3793.0
30	5049.0
31	7228.0
32	9613.0
33	12835.0
34	19009.0
35	28052.0
36	45418.0
37	85459.0
38	206369.0
39	604281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84680864220792	17.658823904530287	11.531672024325413	26.962695428936385
2	19.266797878579702	19.914161514328477	33.69170224751127	27.12733835958055
3	20.283448651019793	20.661335095708367	28.884689077043035	30.170527176228806
4	15.079321369666859	14.603364165234254	32.72017368669726	37.59714077840163
5	16.669420377654554	34.437136643004074	32.10875322594827	16.784689753393103
6	36.41816598983349	32.98530291303974	15.063861939559425	15.53266915756734
7	31.356748572659804	29.060056987324234	19.722078095243617	19.861116344772345
8	28.326313785850175	31.6705750038407	19.54603383489522	20.457077375413903
9	27.593343555881493	13.713094233922435	18.870166874885864	39.82339533531021
10	18.373822546999083	25.291627655761005	30.990070214799122	25.344479582440794
11	37.967587372351005	20.60674398314149	20.083249031128528	21.342419613378976
12	23.906655961011317	24.368409814032717	27.08627424835768	24.638659976598287
13	31.36766679517318	18.03796642791509	24.024437494142326	26.5699292827694
14	24.12869202592933	19.332693699412637	23.15426482197017	33.384349452687864
15	26.587997491707466	26.094261942651308	20.863177280048543	26.45456328559268
16	26.786168061397124	24.419715797701762	22.46786612520013	26.326250015700985
17	25.3566538836504	25.154715077872048	23.641140248916148	25.847490789561405
18	26.03580597255758	23.156680357924454	24.90040745260477	25.907106216913196
19	26.195907695607684	23.67891923124119	24.39053612337398	25.73463694977714
20	26.19919282450552	23.449539937022145	24.429861048709768	25.921406189762575
21	27.44531951260282	23.479879068607985	24.30637885072664	24.768422568062558
22	27.22183412611223	23.23262480832722	23.346638105369543	26.198902960191
23	25.841983367585637	23.440360900395856	24.26396203936937	26.45369369264914
24	25.71801806241165	23.799116300326485	23.899989081777488	26.58287655548438
25	26.336202023832644	23.330695568071253	24.114488674518125	26.21861373357798
26	26.394271508173688	23.761337318001445	23.756602867531043	26.087788306293824
27	26.341999310122933	23.118031782655873	24.111976517125665	26.42799239009553
28	25.42390240461773	23.41852445536911	24.79586305650325	26.36171008350991
29	25.49888064063878	23.654570628821983	24.171012215848428	26.675536514690805
30	24.790935363156507	23.993422012489287	25.304864792790493	25.910777831563713
31	26.34914929654762	23.56567890570424	23.876896558054508	26.208275239693634
32	25.34554241826068	23.276104455504377	24.472181238628863	26.906171887606078
33	25.28612023378523	23.044985975398248	24.8661068420539	26.80278694876262
34	26.13474632524515	22.599947631180513	24.826105566650917	26.439200476923418
35	25.404964602736126	22.857540385345622	25.584873720611384	26.152621291306872
36	24.885527751126364	22.433758757525602	26.045178252060207	26.635535239287822
37	25.34612214688971	22.04592030470537	26.086725470473937	26.52123207793099
38	24.53073383016077	21.44986265262564	27.322610286125066	26.696793231088527
39	24.982052567859654	21.189178012439044	27.454401927790933	26.374367491910373
40	24.28232011262195	22.241385474126226	27.272367138275904	26.203927274975918
41	23.944338321898186	21.580494837033452	28.306892876777713	26.168273964290652
42	23.822305445487636	21.852290942609763	28.247277449425923	26.07812616247668
43	22.40042436135645	21.575084036495852	28.381967734236934	27.64252386791076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	338.0
1	263.5
2	189.0
3	373.0
4	557.0
5	557.0
6	599.0
7	641.0
8	566.0
9	491.0
10	717.5
11	944.0
12	944.0
13	1501.5
14	2059.0
15	2971.0
16	3883.0
17	3593.0
18	3303.0
19	3303.0
20	3788.0
21	4273.0
22	3832.5
23	3392.0
24	4039.0
25	4686.0
26	4686.0
27	5541.5
28	6397.0
29	8228.0
30	10059.0
31	12187.5
32	14316.0
33	14316.0
34	18582.0
35	22848.0
36	28125.5
37	33403.0
38	38545.0
39	43687.0
40	43687.0
41	47882.5
42	52078.0
43	55490.5
44	58903.0
45	65502.5
46	72102.0
47	72102.0
48	75748.0
49	79394.0
50	82160.0
51	84926.0
52	86004.5
53	87083.0
54	87083.0
55	85262.5
56	83442.0
57	80574.5
58	77707.0
59	74419.0
60	71131.0
61	71131.0
62	66143.5
63	61156.0
64	55347.5
65	49539.0
66	44084.5
67	38630.0
68	38630.0
69	31353.5
70	24077.0
71	20317.0
72	16557.0
73	12598.0
74	8639.0
75	8639.0
76	6569.0
77	4499.0
78	3708.5
79	2918.0
80	2634.5
81	2351.0
82	2351.0
83	1956.5
84	1562.0
85	1403.0
86	1244.0
87	1126.0
88	1008.0
89	1008.0
90	728.5
91	449.0
92	259.0
93	69.0
94	49.0
95	29.0
96	29.0
97	17.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1034967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.99723686743223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.48669132268162	39.706410442377
2	7.198980907571087	6.766644218344813
3	2.518927974052499	3.5514796394563937
4	1.301732359642139	2.4471129617641236
5	0.8325084236976286	1.956279779132504
6	0.5558721027255867	1.5674671727875213
7	0.40276461119899054	1.3250176684036745
8	0.30032091176548104	1.1291402421188421
9	0.24570581937796163	1.0392745133711325
>10	1.7538502869899553	16.45040922590636
>50	0.23386395969083595	7.637111245963207
>100	0.16279030679063416	14.189999593813923
>500	0.004958080399112503	1.5053809598875019
>1k	0.0010329334164817716	0.7282723366730829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2014	0.19459557647731762	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1661	0.16048820880279274	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1431	0.13826527802335728	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	1226	0.11845788319820826	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	1169	0.11295046122243513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.662143817145861E-5	0.0	0.0	0.0
6	0.0	9.662143817145861E-5	0.0	0.0	0.0
7	0.0	9.662143817145861E-5	0.0	0.0	0.0
8	0.0	9.662143817145861E-5	0.0	0.0	0.0
9	0.0	9.662143817145861E-5	0.0	9.662143817145861E-5	0.0
10	0.0	9.662143817145861E-5	0.0	1.9324287634291722E-4	9.662143817145861E-5
11	0.0	9.662143817145861E-5	0.0	2.8986431451437583E-4	1.9324287634291722E-4
12	0.0	9.662143817145861E-5	0.0	2.8986431451437583E-4	1.9324287634291722E-4
13	0.0	9.662143817145861E-5	0.0	2.8986431451437583E-4	1.9324287634291722E-4
14	0.0	9.662143817145861E-5	0.0	2.8986431451437583E-4	1.9324287634291722E-4
15	0.0	9.662143817145861E-5	0.0	3.8648575268583445E-4	1.9324287634291722E-4
16	0.0	9.662143817145861E-5	0.0	8.695929435431275E-4	1.9324287634291722E-4
17	0.0	9.662143817145861E-5	0.0	9.662143817145861E-4	1.9324287634291722E-4
18	0.0	9.662143817145861E-5	0.0	0.001256078696228962	1.9324287634291722E-4
19	0.0	9.662143817145861E-5	0.0	0.0015459430107433378	1.9324287634291722E-4
20	0.0	9.662143817145861E-5	0.0	0.0019324287634291722	1.9324287634291722E-4
21	0.0	9.662143817145861E-5	0.0	0.002512157392457924	1.9324287634291722E-4
22	0.0	9.662143817145861E-5	0.0	0.00347837177417251	1.9324287634291722E-4
23	0.0	9.662143817145861E-5	0.0	0.005024314784915848	1.9324287634291722E-4
24	0.0	9.662143817145861E-5	0.0	0.008599307997259817	1.9324287634291722E-4
25	0.0	9.662143817145861E-5	0.0	0.01178781545691795	1.9324287634291722E-4
26	9.662143817145861E-5	9.662143817145861E-5	0.0	0.019710773386977555	1.9324287634291722E-4
27	9.662143817145861E-5	9.662143817145861E-5	0.0	0.06087150604801892	1.9324287634291722E-4
28	9.662143817145861E-5	9.662143817145861E-5	0.0	0.22309890073789793	1.9324287634291722E-4
29	9.662143817145861E-5	9.662143817145861E-5	0.0	0.4395309222419652	1.9324287634291722E-4
30	9.662143817145861E-5	9.662143817145861E-5	0.0	0.7206026858827382	1.9324287634291722E-4
31	9.662143817145861E-5	9.662143817145861E-5	0.0	1.3828460231099156	2.8986431451437583E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAA	45	0.0038255549	20.555555	29
TAAGCTA	45	0.0038255549	20.555555	33
CTAAACA	55	5.142619E-4	20.181818	4
CAGTACT	95	1.6757076E-7	19.473684	4
GGTATCA	830	0.0	19.391567	1
GTCTAGA	330	0.0	18.5	1
AGTGCGC	90	2.1517953E-6	18.5	29
GCCTAAA	50	0.0070344196	18.5	1
TAGGCCG	80	1.6164166E-5	18.5	5
TCTAGAC	355	0.0	18.239437	3
GTGTAAG	145	1.546141E-10	17.86207	1
AAGACGG	280	0.0	17.839285	5
CTAGACA	375	0.0	17.759998	4
CGAGCCG	315	0.0	17.619047	15
GTGCGCT	95	3.6056845E-6	17.526316	30
GTTCTAG	375	0.0	17.266666	1
TACTATA	75	2.0670157E-4	17.266666	2
ACATGTA	260	0.0	17.076925	8
GCACTAT	65	0.0015797897	17.076925	13
AACTATG	185	1.8189894E-12	17.000002	7
>>END_MODULE