FastQCFastQC Report
Fri 10 Feb 2017
ERR1631438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141280
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42022.9742355605889013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27531.94861268403171No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27501.9464892412231032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.690118912797282No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA7640.5407701019252549No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT4320.30577576443941107No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT4000.28312570781426954No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA3890.2753397508493771No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3690.26118346545866367No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT3430.24278029445073615No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.23924122310305776No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT3320.2349943374858437No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT3220.22791619479048697No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.2272083805209513No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA3170.2243771234428086No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA3140.2222536806342016No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT2840.20101925254813138No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2650.18757078142695355No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT2570.18190826727066817No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.17766138165345413No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC2460.17412231030577577No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC2430.17199886749716875No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.1705832389580974No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2350.16633635334088334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.1571347678369196No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA2180.15430351075877688No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.15288788221970553No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT2140.15147225368063422No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2110.14934881087202717No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA1980.14014722536806343No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT1960.13873159682899208No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1930.13660815402038506No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT1880.13306908267270667No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA1870.132361268403171No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT1860.13165345413363533No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1840.13023782559456398No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.1295300113250283No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT1830.1295300113250283No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1820.12882219705549264No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.1274065685164213No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.12315968289920724No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT1710.12103624009060021No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT1710.12103624009060021No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1630.11537372593431483No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT1590.11254246885617214No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1590.11254246885617214No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1570.1111268403171008No Hit
TATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATACA1560.11041902604756512No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT1530.10829558323895809No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACA1450.1026330690826727No Hit
GTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATAC1440.10192525481313704No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTC250.005480238229.63
CAACTGC250.005480238229.65
TATTGAC950.029.21052730
ATTGACT851.8189894E-1228.29411931
GATACAA405.90388E-527.751
ATACAAC358.830776E-426.4285722
AGGGCAA751.3520548E-824.66666810
TCGCTCT451.3152025E-424.6666664
GAGGGCA802.6831913E-823.1259
TACAACT400.001922858523.1253
AGAGGGC903.756213E-922.611118
ATTCCCA753.6929305E-722.2000036
GCAGTCG6050.022.016539
GGCAGTC6100.021.8360658
GGGCAGT6100.021.8360657
TGGGCAG6100.021.8360656
CAGTCGG6100.021.53278510
AGTCGGT6100.021.53278511
CGCTCTG450.003809466720.5555555
CTGATGG450.003809466720.5555559
Produced by FastQC (version 0.11.5)