##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631438.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141280
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7804855605889	34.0	31.0	34.0	31.0	34.0
2	32.880436013590035	34.0	33.0	34.0	31.0	34.0
3	32.87676953567384	34.0	33.0	34.0	31.0	34.0
4	36.32352066817667	37.0	37.0	37.0	35.0	37.0
5	36.357934597961496	37.0	37.0	37.0	35.0	37.0
6	36.46870753114383	37.0	37.0	37.0	35.0	37.0
7	36.465437429218575	37.0	37.0	37.0	35.0	37.0
8	36.49263165345413	37.0	37.0	37.0	35.0	37.0
9	38.31911098527746	39.0	39.0	39.0	37.0	39.0
10	38.30162797281993	39.0	39.0	39.0	37.0	39.0
11	38.359576727066816	39.0	39.0	39.0	37.0	39.0
12	38.148945356738395	39.0	39.0	39.0	37.0	39.0
13	38.2786593997735	39.0	39.0	39.0	37.0	39.0
14	39.60890430351076	41.0	39.0	41.0	37.0	41.0
15	39.534944790486975	41.0	39.0	41.0	37.0	41.0
16	39.4681412797282	40.0	39.0	41.0	37.0	41.0
17	39.54719705549264	40.0	39.0	41.0	37.0	41.0
18	39.75018403171008	41.0	40.0	41.0	38.0	41.0
19	39.8089963193658	41.0	40.0	41.0	38.0	41.0
20	39.84468431483579	41.0	40.0	41.0	38.0	41.0
21	39.81599660249151	41.0	40.0	41.0	38.0	41.0
22	39.7347536806342	41.0	40.0	41.0	37.0	41.0
23	39.644684314835786	41.0	40.0	41.0	37.0	41.0
24	39.61754671574179	41.0	40.0	41.0	37.0	41.0
25	39.58960220838052	41.0	40.0	41.0	36.0	41.0
26	39.475233578708945	41.0	39.0	41.0	36.0	41.0
27	39.344889580973955	41.0	39.0	41.0	35.0	41.0
28	39.188774065685166	41.0	39.0	41.0	35.0	41.0
29	38.98522791619479	40.0	38.0	41.0	35.0	41.0
30	38.752937429218576	40.0	38.0	41.0	35.0	41.0
31	38.46716449603624	40.0	37.0	41.0	35.0	41.0
32	38.25042468856172	40.0	37.0	41.0	35.0	41.0
33	38.030839467723666	40.0	35.0	41.0	35.0	41.0
34	37.867766138165344	40.0	35.0	41.0	35.0	41.0
35	37.66405719139298	40.0	35.0	41.0	35.0	41.0
36	37.48271517553794	40.0	35.0	41.0	34.0	41.0
37	37.28453425821065	40.0	35.0	41.0	33.0	41.0
38	37.07394535673839	40.0	35.0	41.0	33.0	41.0
39	36.78199320498301	40.0	35.0	41.0	33.0	41.0
40	36.526642129105326	40.0	35.0	41.0	32.0	41.0
41	36.21057474518686	40.0	35.0	41.0	30.0	41.0
42	35.87127689694224	40.0	35.0	41.0	25.0	41.0
43	34.925247734994336	39.0	35.0	41.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	2.0
14	5.0
15	3.0
16	4.0
17	3.0
18	5.0
19	6.0
20	12.0
21	22.0
22	47.0
23	67.0
24	124.0
25	211.0
26	341.0
27	516.0
28	911.0
29	1234.0
30	1654.0
31	1938.0
32	2160.0
33	2809.0
34	3727.0
35	5037.0
36	8111.0
37	21029.0
38	26817.0
39	64480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.4503114382786	14.84144960362401	18.881653454133634	19.82658550396376
2	19.265996602491505	23.398924122310305	35.44309173272933	21.891987542468854
3	18.70753114382786	26.127548131370325	33.02661381653454	22.13830690826727
4	11.416336353340883	20.380804077010193	32.34569648924122	35.8571630804077
5	11.289637599093998	41.21531710079275	30.435305775764437	17.05973952434881
6	24.396942242355607	35.26613816534541	20.369479048697624	19.96744054360136
7	25.066534541336356	35.185447338618346	18.05634201585504	21.69167610419026
8	33.57375424688562	29.73456964892412	17.91548697621744	18.77618912797282
9	30.03114382785957	12.438420158550397	17.335079275198186	40.195356738391844
10	20.32701019252548	29.393403171007925	22.54459229898075	27.73499433748584
11	31.560022650056624	26.58691959229898	15.27675537938845	26.576302378255946
12	23.019535673839183	35.85362400906002	23.75849377123443	17.368346545866366
13	34.837910532276325	20.016987542468854	26.0971121177803	19.04798980747452
14	24.067100792751983	22.272083805209515	28.735843714609288	24.924971687429217
15	29.142129105322763	26.061013590033976	23.804501698754247	20.992355605889017
16	19.289354473386183	28.00537938844847	27.687570781426952	25.01769535673839
17	15.014156285390715	33.59357304643262	27.326585503963756	24.065685164212912
18	18.96446772366931	24.12514156285391	33.247451868629675	23.662938844847112
19	20.828850509626275	29.098952434881088	33.59852774631937	16.47366930917327
20	24.890996602491505	22.722253680634203	35.56908267270668	16.81766704416761
21	29.859852774631936	21.269110985277464	30.947055492638732	17.923980747451868
22	26.26911098527746	23.207814269535675	28.26868629671574	22.254388448471122
23	22.044875424688563	25.633493771234427	28.991364665911668	23.330266138165346
24	19.059314835787088	26.41492072480181	34.74943374858437	19.776330690826725
25	18.382644394110986	25.76727066817667	32.09159116647792	23.75849377123443
26	23.51429784824462	23.97579275198188	30.901047565118912	21.608861834654586
27	21.51967723669309	25.12599093997735	30.035390713476783	23.318941109852776
28	18.81511891279728	24.731030577576444	35.06228765571914	21.391562853907136
29	19.82800113250283	25.606596828992075	35.60942808607022	18.95597395243488
30	19.152038505096264	25.80407701019253	36.332814269535675	18.71107021517554
31	19.517978482446203	27.5615798414496	32.79515855039637	20.125283125707814
32	18.169592298980746	26.77165911664779	36.893403171007925	18.165345413363536
33	19.164779161947905	25.15713476783692	37.40656851642129	18.271517553793885
34	19.556200453001132	25.621460928652322	32.49575311438279	22.326585503963763
35	16.859428086070217	26.787938844847115	33.4187429218573	22.93389014722537
36	14.974518686296715	25.780719139297847	34.37995469988675	24.864807474518685
37	15.557049830124575	25.734711211778027	36.00934314835787	22.698895809739525
38	16.404303510758776	22.45753114382786	37.58989241223103	23.548272933182332
39	16.14028878822197	23.030860702151756	39.61636466591166	21.21248584371461
40	15.359569648924124	23.48032276330691	37.010900339750854	24.14920724801812
41	12.597678369195922	25.400622876557193	39.1350509626274	22.866647791619478
42	12.310305775764439	24.825169875424688	37.58210645526614	25.282417893544736
43	11.114807474518686	27.78949603624009	38.23754246885617	22.85815402038505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	18.0
2	32.0
3	208.0
4	384.0
5	384.0
6	550.0
7	716.0
8	744.0
9	772.0
10	1131.5
11	1491.0
12	1491.0
13	2447.5
14	3404.0
15	5520.0
16	7636.0
17	6630.5
18	5625.0
19	5625.0
20	5701.5
21	5778.0
22	3991.0
23	2204.0
24	1919.0
25	1634.0
26	1634.0
27	1556.0
28	1478.0
29	1543.5
30	1609.0
31	1717.0
32	1825.0
33	1825.0
34	1948.5
35	2072.0
36	2261.0
37	2450.0
38	3026.0
39	3602.0
40	3602.0
41	4807.0
42	6012.0
43	7690.5
44	9369.0
45	11556.0
46	13743.0
47	13743.0
48	15704.0
49	17665.0
50	15918.0
51	14171.0
52	11525.5
53	8880.0
54	8880.0
55	7515.0
56	6150.0
57	5553.5
58	4957.0
59	4541.0
60	4125.0
61	4125.0
62	3795.0
63	3465.0
64	3146.5
65	2828.0
66	2571.0
67	2314.0
68	2314.0
69	2019.5
70	1725.0
71	1514.0
72	1303.0
73	1087.5
74	872.0
75	872.0
76	689.5
77	507.0
78	385.0
79	263.0
80	198.5
81	134.0
82	134.0
83	93.0
84	52.0
85	34.0
86	16.0
87	13.5
88	11.0
89	11.0
90	6.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	141280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.014014722536814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5487256371814	53.4364382785957
2	4.723238380809596	5.574745186862967
3	1.5772113943028485	2.792327293318233
4	0.7580209895052474	1.7893544733861833
5	0.46296851574212894	1.3660815402038504
6	0.30704647676161917	1.087202718006795
7	0.23508245877061468	0.9711211778029446
8	0.1823088455772114	0.8607021517553795
9	0.13193403298350825	0.7007361268403172
>10	0.8947526236881559	10.2519818799547
>50	0.09355322338830585	3.7783125707814267
>100	0.07916041979010495	9.290770101925256
>500	0.0023988005997001498	1.2308890147225369
>1k	0.003598200899550225	6.869337485843714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4202	2.9742355605889013	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2753	1.94861268403171	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2750	1.9464892412231032	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	975	0.690118912797282	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	764	0.5407701019252549	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	432	0.30577576443941107	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	400	0.28312570781426954	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	389	0.2753397508493771	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	369	0.26118346545866367	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	343	0.24278029445073615	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.23924122310305776	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	332	0.2349943374858437	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	322	0.22791619479048697	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	321	0.2272083805209513	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	317	0.2243771234428086	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	314	0.2222536806342016	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	284	0.20101925254813138	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	265	0.18757078142695355	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	257	0.18190826727066817	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	251	0.17766138165345413	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	246	0.17412231030577577	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	243	0.17199886749716875	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.1705832389580974	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	235	0.16633635334088334	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.1571347678369196	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	218	0.15430351075877688	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	216	0.15288788221970553	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	214	0.15147225368063422	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	211	0.14934881087202717	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	198	0.14014722536806343	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	196	0.13873159682899208	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	193	0.13660815402038506	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	188	0.13306908267270667	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	187	0.132361268403171	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	186	0.13165345413363533	No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	184	0.13023782559456398	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.1295300113250283	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	183	0.1295300113250283	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	182	0.12882219705549264	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	180	0.1274065685164213	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.12315968289920724	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	171	0.12103624009060021	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	171	0.12103624009060021	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	163	0.11537372593431483	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	159	0.11254246885617214	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	159	0.11254246885617214	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	157	0.1111268403171008	No Hit
TATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATACA	156	0.11041902604756512	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	153	0.10829558323895809	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACA	145	0.1026330690826727	No Hit
GTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATAC	144	0.10192525481313704	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.010617214043035108	0.0
4	0.0	0.0	0.0	0.010617214043035108	0.0
5	0.0	0.0	0.0	0.011325028312570781	0.0
6	0.0	0.0	0.0	0.011325028312570781	0.0
7	0.0	0.0	0.0	0.011325028312570781	0.0
8	0.0	0.0	0.0	0.02194224235560589	0.0
9	0.0	0.0	0.0	0.023357870894677238	0.0
10	0.0	0.0	0.0	0.036098527746319366	0.0
11	0.0	0.0	0.0	0.048839184597961494	0.0
12	0.0	0.0	0.0	0.05167044167610419	0.0
13	0.0	0.0	0.0	0.05450169875424689	0.0
14	0.0	0.0	0.0	0.05733295583238958	0.0
15	0.0	0.0	0.0	0.06511891279728199	0.0
16	0.0	0.0	0.0	0.0785673839184598	0.0
17	0.0	0.0	0.0	0.08989241223103057	0.0
18	0.0	0.0	0.0	0.09130804077010192	0.0
19	0.0	0.0	0.0	0.10050962627406569	0.0
20	0.0	0.0	0.0	0.10758776896942242	0.0
21	0.0	0.0	0.0	0.1245753114382786	0.0
22	0.0	0.0	0.0	0.1543035107587769	0.0
23	0.0	0.0	0.0	0.19323329558323896	0.0
24	0.0	0.0	0.0	0.2746319365798415	0.0
25	0.0	0.0	0.0	0.30152887882219703	0.0
26	0.0	0.0	0.0	0.34541336353340885	0.0
27	0.0	0.0	0.0	0.45937146092865233	0.0
28	0.0	0.0	0.0	0.9215741789354474	0.0
29	0.0	0.0	0.0	1.8374858437146093	0.0
30	0.0	0.0	0.0	3.6077293318233297	0.0
31	0.0	0.0	0.0	6.214609286523216	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCTC	25	0.0054802382	29.6	3
CAACTGC	25	0.0054802382	29.6	5
TATTGAC	95	0.0	29.210527	30
ATTGACT	85	1.8189894E-12	28.294119	31
GATACAA	40	5.90388E-5	27.75	1
ATACAAC	35	8.830776E-4	26.428572	2
AGGGCAA	75	1.3520548E-8	24.666668	10
TCGCTCT	45	1.3152025E-4	24.666666	4
GAGGGCA	80	2.6831913E-8	23.125	9
TACAACT	40	0.0019228585	23.125	3
AGAGGGC	90	3.756213E-9	22.61111	8
ATTCCCA	75	3.6929305E-7	22.200003	6
GCAGTCG	605	0.0	22.01653	9
GGCAGTC	610	0.0	21.836065	8
GGGCAGT	610	0.0	21.836065	7
TGGGCAG	610	0.0	21.836065	6
CAGTCGG	610	0.0	21.532785	10
AGTCGGT	610	0.0	21.532785	11
CGCTCTG	45	0.0038094667	20.555555	5
CTGATGG	45	0.0038094667	20.555555	9
>>END_MODULE