FastQCFastQC Report
Fri 10 Feb 2017
ERR1631437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686627
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.22457607988034262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13610.19821533379840872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11890.1731653430465158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG250.005494822329.59999813
GGTATCA6350.026.2204741
CTTACCG400.001930400123.1259
ATACACA4100.019.8536637
GACGGAC1254.110916E-1019.247
CGTATTA500.00703206918.49999815
TATATAG500.00703206918.4999982
TCGCACG852.7205297E-517.41176422
TAAGTTA852.7205297E-517.4117645
TAGACTA650.001579057917.0769235
GTCCTAT1950.017.0769231
AGTACCG650.001579057917.0769235
GTATAGA1107.796334E-716.8181821
TATACAC4950.016.44444536
ACACTGG1151.240338E-616.0869566
GCGAAAG1504.658432E-916.03333318
CGGACCA1851.8189894E-1116.09
TCTAGCG1403.4655386E-815.85714228
TACGAGT700.002591068415.8571424
GTACTAT700.002591068415.8571421
Produced by FastQC (version 0.11.5)