##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631437.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686627
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82295190838694	34.0	31.0	34.0	31.0	34.0
2	32.99092520393168	34.0	33.0	34.0	31.0	34.0
3	33.10601389109371	34.0	33.0	34.0	31.0	34.0
4	36.42443277063092	37.0	37.0	37.0	35.0	37.0
5	36.40100520369866	37.0	37.0	37.0	35.0	37.0
6	36.43932003839057	37.0	37.0	37.0	35.0	37.0
7	36.446951547201024	37.0	37.0	37.0	35.0	37.0
8	36.44279790919961	37.0	37.0	37.0	35.0	37.0
9	38.29670257650806	39.0	39.0	39.0	37.0	39.0
10	38.256919695846506	39.0	39.0	39.0	37.0	39.0
11	38.309993635554676	39.0	39.0	39.0	37.0	39.0
12	38.095316671205765	39.0	39.0	39.0	37.0	39.0
13	38.225476131873634	39.0	39.0	39.0	37.0	39.0
14	39.754362994755525	41.0	40.0	41.0	38.0	41.0
15	39.742857475747385	41.0	40.0	41.0	38.0	41.0
16	39.720567353162636	41.0	40.0	41.0	38.0	41.0
17	39.71618360478105	41.0	40.0	41.0	38.0	41.0
18	39.69939574179285	41.0	40.0	41.0	37.0	41.0
19	39.71385046029358	41.0	40.0	41.0	38.0	41.0
20	39.66443352795623	41.0	40.0	41.0	37.0	41.0
21	39.64412847149908	41.0	40.0	41.0	37.0	41.0
22	39.58594695518819	41.0	40.0	41.0	37.0	41.0
23	39.53402211098602	41.0	40.0	41.0	37.0	41.0
24	39.501483338115165	41.0	39.0	41.0	37.0	41.0
25	39.506430711288665	41.0	39.0	41.0	37.0	41.0
26	39.4148089137188	41.0	39.0	41.0	36.0	41.0
27	39.36138835204558	41.0	39.0	41.0	36.0	41.0
28	39.31288021007039	41.0	39.0	41.0	36.0	41.0
29	39.253134525732314	41.0	39.0	41.0	36.0	41.0
30	39.21509203687009	41.0	39.0	41.0	36.0	41.0
31	39.165442081362954	41.0	39.0	41.0	35.0	41.0
32	39.103272956059115	41.0	39.0	41.0	35.0	41.0
33	39.02311735483749	40.0	39.0	41.0	35.0	41.0
34	38.985762284326135	40.0	39.0	41.0	35.0	41.0
35	38.908255865266	40.0	39.0	41.0	35.0	41.0
36	38.86548300605715	40.0	39.0	41.0	35.0	41.0
37	38.80938850351064	40.0	38.0	41.0	35.0	41.0
38	38.72949359696021	40.0	38.0	41.0	35.0	41.0
39	38.65771372229755	40.0	38.0	41.0	35.0	41.0
40	38.583222040496516	40.0	38.0	41.0	35.0	41.0
41	38.504247575466735	40.0	38.0	41.0	35.0	41.0
42	38.413027742864756	40.0	38.0	41.0	35.0	41.0
43	37.52940097025022	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	2.0
15	2.0
16	3.0
17	7.0
18	11.0
19	27.0
20	47.0
21	99.0
22	134.0
23	257.0
24	406.0
25	682.0
26	1071.0
27	1414.0
28	2171.0
29	2910.0
30	4063.0
31	5365.0
32	6999.0
33	9653.0
34	14065.0
35	20083.0
36	31586.0
37	58767.0
38	140422.0
39	386379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.189679403810224	18.50422427314976	12.305371038424065	26.000725284615957
2	19.278589394241706	20.29952215686246	33.7544256197324	26.66746282916343
3	19.653902337076754	22.429936486622285	29.159208711571203	28.756952464729757
4	14.455155419172272	15.908928719668758	34.31673965631995	35.31917620483902
5	15.724549136576336	35.12707772924747	32.934038422607905	16.21433471156829
6	33.619709099700415	34.83099266413934	16.172099262044746	15.377198974115494
7	30.020520602889196	29.883036932716017	20.95606493773184	19.140377526662945
8	28.076670448438527	32.073600368176606	19.84949616021508	20.000233023169788
9	27.31395648583583	14.489963255158914	19.598850613215035	38.597229645790215
10	18.593501275073656	25.593371655935464	31.319916053403084	24.493211015587793
11	36.54749958856846	21.576634766765654	20.844650734678364	21.03121490998752
12	22.912148808596225	25.489239426937772	27.575524993919554	24.023086770546453
13	30.759495330070035	18.999107229980762	24.264848309198445	25.976549130750758
14	23.680397071481313	19.954065307656123	24.435100862622647	31.930436758239917
15	26.232292059589852	26.522405905972242	21.578673719501275	25.66662831493664
16	25.798286405865195	25.0671762106646	23.56475932347548	25.56977805999473
17	24.52277583025427	25.786198328932592	24.500201710681345	25.19082413013179
18	24.604479579160156	24.422721506727814	25.734205034174305	25.238593879937728
19	25.447440895857575	24.523649667140965	25.582157415889554	24.4467520211119
20	25.60458589598137	24.318880556692353	25.186309306217204	24.890224241109074
21	26.506094284087283	24.3314055520683	24.464665677289123	24.697834486555294
22	26.508424515785133	24.078138494408172	24.58452697024731	24.828910019559384
23	25.434770261000516	24.201786413875364	24.910031210540804	25.45341211458332
24	24.916002429266545	24.828764380078265	24.568797906286818	25.68643528436837
25	25.474238560382855	23.840309221746306	25.021882332037627	25.66356988583321
26	24.91687626615324	24.708757447638966	25.14247182240139	25.231894463806405
27	25.27602322658445	24.42869272545356	24.715748142732515	25.579535905229477
28	24.74516731791788	24.42592557531236	25.824938430909363	25.0039686758604
29	24.68720280443385	24.4653938746947	25.835424473549683	25.011978847321764
30	24.478355788513998	24.926051553463523	25.27849909776341	25.317093560259064
31	25.076642776937113	24.496415084172337	25.05435993632642	25.372582202564132
32	24.28407272070571	24.26266371698171	25.465354552034803	25.987909010277775
33	24.030805663045584	24.05600129327859	26.033348528385865	25.879844515289964
34	25.04562156745948	23.3509605651977	26.014706674803058	25.588711192539765
35	24.689387396650584	23.466015755279066	26.54512566502628	25.29947118304407
36	24.087459421199576	23.71709822072246	26.11592611417844	26.079516243899526
37	24.043185018940413	22.728060504466036	26.93427435856732	26.294480118026236
38	23.494124175134388	22.658007914049406	27.828209493655216	26.01965841716099
39	23.339309406708445	22.37852574978846	28.327170355957453	25.95499448754564
40	23.03332085688445	22.37007865988375	28.98196546305345	25.61463502017835
41	22.203904011930785	22.43212107883902	29.484712951864694	25.879261957365497
42	22.073119757888925	22.75733404017028	29.41203885078798	25.75750735115281
43	21.452113010411765	22.05753633340955	29.837306135645704	26.65304452053298
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	169.0
1	160.0
2	151.0
3	282.0
4	413.0
5	413.0
6	490.0
7	567.0
8	482.5
9	398.0
10	607.0
11	816.0
12	816.0
13	1307.5
14	1799.0
15	2502.0
16	3205.0
17	3101.5
18	2998.0
19	2998.0
20	3431.0
21	3864.0
22	3641.5
23	3419.0
24	4062.5
25	4706.0
26	4706.0
27	5672.5
28	6639.0
29	8554.0
30	10469.0
31	12504.5
32	14540.0
33	14540.0
34	17746.5
35	20953.0
36	24417.0
37	27881.0
38	31607.0
39	35333.0
40	35333.0
41	38439.0
42	41545.0
43	44069.0
44	46593.0
45	48195.0
46	49797.0
47	49797.0
48	51111.0
49	52425.0
50	52613.5
51	52802.0
52	52542.0
53	52282.0
54	52282.0
55	50169.5
56	48057.0
57	45326.5
58	42596.0
59	40971.5
60	39347.0
61	39347.0
62	36166.5
63	32986.0
64	29624.0
65	26262.0
66	23034.0
67	19806.0
68	19806.0
69	17212.0
70	14618.0
71	12708.0
72	10798.0
73	8691.5
74	6585.0
75	6585.0
76	5359.0
77	4133.0
78	3488.0
79	2843.0
80	2381.5
81	1920.0
82	1920.0
83	1556.0
84	1192.0
85	1025.0
86	858.0
87	704.0
88	550.0
89	550.0
90	395.0
91	240.0
92	142.5
93	45.0
94	34.0
95	23.0
96	23.0
97	13.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	686627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.36791546831358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.71687223811455	60.44038665018792
2	5.529768177397013	7.450579102685049
3	1.582064072672902	3.19741076139852
4	0.7624254008184249	2.05452039812923
5	0.46185195828447434	1.555700184229178
6	0.30161001478010097	1.2191302788061595
7	0.24154752541715566	1.139078729171829
8	0.16839216085301362	0.9075383086297989
9	0.12981447001113014	0.7870797218046369
>10	0.9751061736831921	12.685944606457236
>50	0.09861694166796568	4.589651889088129
>100	0.031061999189968808	3.290475657508618
>500	2.1721677753635976E-4	0.08370328542215047
>1k	6.516503326090792E-4	0.598800426481538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1542	0.22457607988034262	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1361	0.19821533379840872	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1189	0.1731653430465158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.4563948111565668E-4	0.0
6	0.0	0.0	0.0	1.4563948111565668E-4	0.0
7	0.0	0.0	0.0	1.4563948111565668E-4	0.0
8	0.0	0.0	0.0	1.4563948111565668E-4	0.0
9	0.0	0.0	0.0	5.825579244626267E-4	0.0
10	0.0	0.0	0.0	5.825579244626267E-4	0.0
11	0.0	0.0	0.0	5.825579244626267E-4	0.0
12	0.0	0.0	0.0	0.0010194763678095969	0.0
13	0.0	0.0	0.0	0.0011651158489252534	0.0
14	0.0	0.0	0.0	0.0016020342922722236	0.0
15	0.0	0.0	0.0	0.002330231697850507	0.0
16	0.0	0.0	0.0	0.002767150141197477	1.4563948111565668E-4
17	0.0	0.0	0.0	0.0033497080656601035	2.9127896223131336E-4
18	0.0	0.0	0.0	0.003640987027891417	2.9127896223131336E-4
19	0.0	0.0	0.0	0.004223544952354044	2.9127896223131336E-4
20	0.0	0.0	0.0	0.004514823914585357	2.9127896223131336E-4
21	0.0	0.0	0.0	0.005825579244626267	4.3691844334697004E-4
22	0.0	0.0	0.0	0.009757845234748997	4.3691844334697004E-4
23	0.0	1.4563948111565668E-4	0.0	0.012961913819293444	4.3691844334697004E-4
24	0.0	2.9127896223131336E-4	0.0	0.0174767377338788	4.3691844334697004E-4
25	0.0	2.9127896223131336E-4	0.0	0.02228284061069547	4.3691844334697004E-4
26	0.0	2.9127896223131336E-4	0.0	0.030875569996519216	4.3691844334697004E-4
27	0.0	2.9127896223131336E-4	0.0	0.08461653852819653	4.3691844334697004E-4
28	0.0	2.9127896223131336E-4	0.0	0.27554989827082244	4.3691844334697004E-4
29	0.0	2.9127896223131336E-4	0.0	0.5225544582429762	4.3691844334697004E-4
30	0.0	2.9127896223131336E-4	0.0	0.8827208950419951	4.3691844334697004E-4
31	0.0	2.9127896223131336E-4	0.0	1.6062578372245775	4.3691844334697004E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACG	25	0.0054948223	29.599998	13
GGTATCA	635	0.0	26.220474	1
CTTACCG	40	0.0019304001	23.125	9
ATACACA	410	0.0	19.85366	37
GACGGAC	125	4.110916E-10	19.24	7
CGTATTA	50	0.007032069	18.499998	15
TATATAG	50	0.007032069	18.499998	2
TCGCACG	85	2.7205297E-5	17.411764	22
TAAGTTA	85	2.7205297E-5	17.411764	5
TAGACTA	65	0.0015790579	17.076923	5
GTCCTAT	195	0.0	17.076923	1
AGTACCG	65	0.0015790579	17.076923	5
GTATAGA	110	7.796334E-7	16.818182	1
TATACAC	495	0.0	16.444445	36
ACACTGG	115	1.240338E-6	16.086956	6
GCGAAAG	150	4.658432E-9	16.033333	18
CGGACCA	185	1.8189894E-11	16.0	9
TCTAGCG	140	3.4655386E-8	15.857142	28
TACGAGT	70	0.0025910684	15.857142	4
GTACTAT	70	0.0025910684	15.857142	1
>>END_MODULE