FastQCFastQC Report
Fri 10 Feb 2017
ERR1631436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29251
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380.1299100885439814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT370.1264914020033503No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC310.10597928275956378No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA300.10256059621893268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTG200.001812769637.028
TACCCTA250.00541029929.630
CGGTGAT358.663607E-426.4285714
GTCGGTG358.663607E-426.4285712
TATACAC551.821852E-523.54545437
GGTGATT400.001886799923.12515
TGATTCC400.001886799923.12517
GTGATTC400.001886799923.12516
GATTCCT400.001886799923.12518
ATTCCTC400.001886799923.12519
TCGGTGA450.003738704820.55555513
TCTATGG450.003738704820.5555552
TATGGGC554.980358E-420.1818184
CTCTATG500.006876234418.51
CTATGGG608.9468993E-418.53
TTATACA751.981887E-417.26666636
ATCAACG700.002512434715.8571422
AACGCAG750.003978687314.85
TCAACGC750.003978687314.83
TATCAAC750.003978687314.81
Produced by FastQC (version 0.11.5)