##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631436.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29251
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76906772418037	34.0	31.0	34.0	31.0	34.0
2	32.95172814604629	34.0	33.0	34.0	31.0	34.0
3	33.040237940583225	34.0	33.0	34.0	31.0	34.0
4	36.41239615739633	37.0	37.0	37.0	35.0	37.0
5	36.37967932720249	37.0	37.0	37.0	35.0	37.0
6	36.42839561040648	37.0	37.0	37.0	35.0	37.0
7	36.41451574305152	37.0	37.0	37.0	35.0	37.0
8	36.411233803972515	37.0	37.0	37.0	35.0	37.0
9	38.25325629892995	39.0	39.0	39.0	37.0	39.0
10	38.214967009674886	39.0	39.0	39.0	37.0	39.0
11	38.273870978769956	39.0	39.0	39.0	37.0	39.0
12	38.04475060681686	39.0	38.0	39.0	37.0	39.0
13	38.15794331817715	39.0	39.0	39.0	37.0	39.0
14	39.65792622474445	41.0	40.0	41.0	37.0	41.0
15	39.6630542545554	41.0	40.0	41.0	37.0	41.0
16	39.64907182660422	41.0	40.0	41.0	37.0	41.0
17	39.627807596321496	41.0	40.0	41.0	37.0	41.0
18	39.6094150627329	41.0	40.0	41.0	37.0	41.0
19	39.610748350483746	41.0	40.0	41.0	37.0	41.0
20	39.576629858808246	41.0	40.0	41.0	37.0	41.0
21	39.55218625004273	41.0	39.0	41.0	37.0	41.0
22	39.505008375782026	41.0	39.0	41.0	37.0	41.0
23	39.44808724488052	41.0	39.0	41.0	37.0	41.0
24	39.415302040955865	41.0	39.0	41.0	37.0	41.0
25	39.409080031451914	41.0	39.0	41.0	37.0	41.0
26	39.341355851082014	41.0	39.0	41.0	36.0	41.0
27	39.25961505589552	41.0	39.0	41.0	36.0	41.0
28	39.19859150114526	41.0	39.0	41.0	36.0	41.0
29	39.120782195480494	40.0	39.0	41.0	36.0	41.0
30	39.05664763597826	40.0	39.0	41.0	35.0	41.0
31	39.02553758845851	40.0	39.0	41.0	35.0	41.0
32	38.948343646371065	40.0	39.0	41.0	35.0	41.0
33	38.89398653037503	40.0	38.0	41.0	35.0	41.0
34	38.86766264401217	40.0	38.0	41.0	35.0	41.0
35	38.80322040272127	40.0	38.0	41.0	35.0	41.0
36	38.74079518648935	40.0	38.0	41.0	35.0	41.0
37	38.67242145567673	40.0	38.0	41.0	35.0	41.0
38	38.581484393695945	40.0	38.0	41.0	35.0	41.0
39	38.50388020922362	40.0	38.0	41.0	35.0	41.0
40	38.42497692386585	40.0	38.0	41.0	35.0	41.0
41	38.35691087484189	40.0	38.0	41.0	34.0	41.0
42	38.23995760828689	40.0	37.0	41.0	34.0	41.0
43	37.29140884072339	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	0.0
21	6.0
22	4.0
23	16.0
24	23.0
25	23.0
26	40.0
27	53.0
28	74.0
29	127.0
30	175.0
31	236.0
32	292.0
33	461.0
34	622.0
35	951.0
36	1511.0
37	2809.0
38	6841.0
39	14984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71132610850911	16.508837304707534	12.789306348500906	26.99053023828245
2	21.20269392499402	18.6386790195207	32.286075689720015	27.872551365765275
3	20.95996718060921	20.348022289836244	27.811015008033912	30.88099552152063
4	16.41311408156986	15.161874807698883	31.462172233427914	36.96283887730334
5	17.944685651772588	33.0655362209839	31.516871218078013	17.4729069091655
6	36.42952377696489	31.332262144883938	15.442207104030631	16.79600697412054
7	31.602338381593793	27.561450890567844	19.23353047759051	21.602680250247854
8	29.363098697480428	30.976718744658307	18.26262350005128	21.39755905780999
9	27.845201873440224	14.126012785887662	18.173737649994873	39.85504769067724
10	20.23178694745479	24.0709719325835	29.87248299203446	25.82475812792725
11	38.672182147618884	20.36169703599877	18.361765409729582	22.604355406652765
12	24.211138080749375	24.149601723018016	25.906806604902393	25.732453591330213
13	32.833065536220985	17.048989778127243	22.925711941472088	27.192232744179684
14	24.997435985094526	18.871149704283614	22.18385696215514	33.94755734846672
15	28.419541212266246	24.546169361731224	19.937779904960514	27.096509521042016
16	27.482821100133332	23.3120235205634	21.992410515879797	27.21274486342347
17	26.01278588766196	24.676079450275203	22.700078629790436	26.6110560322724
18	26.87087620936036	22.05736556015179	24.050459813339714	27.02129841714813
19	26.966599432498032	23.558168951488838	22.994085672284708	26.481145943728418
20	27.551194830945953	21.982154456257906	23.99917951523025	26.467471197565896
21	29.281050220505282	22.047109500529896	23.09322758196301	25.578612697001812
22	29.09985983385183	21.68131004068237	22.419746333458683	26.79908379200711
23	27.527264025161536	22.11206454480189	22.799220539468738	27.561450890567844
24	26.73412874773512	23.171857372397525	23.16160131277563	26.932412567091724
25	27.223000923045365	22.1906943352364	22.539400362380775	28.046904379337455
26	27.45205292126765	22.720590749034223	22.942805374175244	26.88455095552289
27	27.499914532836485	22.214625141020818	22.93254931455335	27.35291101158935
28	26.785409045844588	22.221462514102083	24.088065365286656	26.905063074766673
29	25.982017708796278	22.74452155481864	24.368397661618406	26.905063074766673
30	25.896550545280505	23.09664626850364	24.590612286759427	26.416190899456428
31	27.052066596013812	22.782127106765582	23.342791699429082	26.823014597791527
32	25.971761649174386	22.30351099107723	24.317117363508938	27.407609996239447
33	25.6982667259239	22.108645858261255	24.97008649276948	27.223000923045365
34	26.487983316809682	21.442001982838192	24.50172643670302	27.568288263649105
35	25.87945711257735	21.308673207753582	25.168370312126083	27.64349936754299
36	24.69317288297836	21.137738880722026	25.992273768418173	28.17681446788144
37	25.527332398892344	19.978804143448087	26.532426241837886	27.961437215821682
38	24.75129055416909	19.243786537212404	27.828108440737072	28.17681446788144
39	24.07780930566476	19.17883149294041	29.356261324399163	27.387097876995657
40	23.527400772623157	19.336091073809442	29.192164370448875	27.944343783118526
41	21.74284639841373	19.57539913165362	30.556220300160682	28.125534169771978
42	21.49670096748829	19.821544562579057	30.761341492598543	27.92041297733411
43	20.54972479573348	19.643772862466243	31.031417729308398	28.77508461249188
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	3.5
4	5.0
5	5.0
6	7.0
7	9.0
8	8.0
9	7.0
10	15.0
11	23.0
12	23.0
13	32.5
14	42.0
15	63.5
16	85.0
17	82.5
18	80.0
19	80.0
20	92.0
21	104.0
22	88.0
23	72.0
24	77.5
25	83.0
26	83.0
27	116.0
28	149.0
29	176.0
30	203.0
31	272.0
32	341.0
33	341.0
34	401.0
35	461.0
36	552.0
37	643.0
38	793.5
39	944.0
40	944.0
41	1099.5
42	1255.0
43	1415.0
44	1575.0
45	1801.0
46	2027.0
47	2027.0
48	2197.0
49	2367.0
50	2399.5
51	2432.0
52	2515.0
53	2598.0
54	2598.0
55	2493.0
56	2388.0
57	2297.0
58	2206.0
59	2143.5
60	2081.0
61	2081.0
62	1963.5
63	1846.0
64	1717.5
65	1589.0
66	1443.0
67	1297.0
68	1297.0
69	1079.0
70	861.0
71	743.5
72	626.0
73	483.5
74	341.0
75	341.0
76	274.5
77	208.0
78	160.0
79	112.0
80	99.0
81	86.0
82	86.0
83	63.0
84	40.0
85	35.5
86	31.0
87	23.5
88	16.0
89	16.0
90	15.0
91	14.0
92	7.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	29251.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.38330313493556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.12447298185897	83.19031827971693
2	3.659149808532859	6.468154934874021
3	0.9592697172475148	2.5435027862295305
4	0.43708660503616603	1.5452463163652526
5	0.2359494062584613	1.0426993948924823
6	0.16245696824353073	0.8615090082390345
7	0.10443662244226976	0.6461317561792759
8	0.06575639190809578	0.4649413695258282
9	0.04641627664100878	0.36921814638815764
>10	0.20500522183112213	2.8682780075894843
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.1299100885439814	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37	0.1264914020033503	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	31	0.10597928275956378	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	30	0.10256059621893268	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0034186865406310896	0.0
12	0.0	0.0	0.0	0.0034186865406310896	0.0
13	0.0	0.0	0.0	0.0034186865406310896	0.0
14	0.0	0.0	0.0	0.0034186865406310896	0.0
15	0.0	0.0	0.0	0.0034186865406310896	0.0
16	0.0	0.0	0.0	0.006837373081262179	0.0
17	0.0	0.0	0.0	0.006837373081262179	0.0
18	0.0	0.0	0.0	0.006837373081262179	0.0
19	0.0	0.0	0.0	0.006837373081262179	0.0
20	0.0	0.0	0.0	0.006837373081262179	0.0
21	0.0	0.0	0.0	0.01025605962189327	0.0
22	0.0	0.0	0.0	0.01709343270315545	0.0
23	0.0	0.0	0.0	0.023930805784417628	0.0
24	0.0	0.0	0.0	0.04102423848757308	0.0
25	0.0	0.0	0.0	0.05469898465009743	0.0
26	0.0	0.0	0.0	0.09230453659703942	0.0
27	0.0	0.0	0.0	0.18802775973470992	0.0
28	0.0	0.0	0.0	0.5298964137978189	0.0
29	0.0	0.0	0.0	0.9640696044579673	0.0
30	0.0	0.0	0.0	1.59994530101535	0.0
31	0.0	0.0	0.0	2.758880038289289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATTG	20	0.0018127696	37.0	28
TACCCTA	25	0.005410299	29.6	30
CGGTGAT	35	8.663607E-4	26.42857	14
GTCGGTG	35	8.663607E-4	26.42857	12
TATACAC	55	1.821852E-5	23.545454	37
GGTGATT	40	0.0018867999	23.125	15
TGATTCC	40	0.0018867999	23.125	17
GTGATTC	40	0.0018867999	23.125	16
GATTCCT	40	0.0018867999	23.125	18
ATTCCTC	40	0.0018867999	23.125	19
TCGGTGA	45	0.0037387048	20.555555	13
TCTATGG	45	0.0037387048	20.555555	2
TATGGGC	55	4.980358E-4	20.181818	4
CTCTATG	50	0.0068762344	18.5	1
CTATGGG	60	8.9468993E-4	18.5	3
TTATACA	75	1.981887E-4	17.266666	36
ATCAACG	70	0.0025124347	15.857142	2
AACGCAG	75	0.0039786873	14.8	5
TCAACGC	75	0.0039786873	14.8	3
TATCAAC	75	0.0039786873	14.8	1
>>END_MODULE