FastQCFastQC Report
Fri 10 Feb 2017
ERR1631435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535004
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.47251983162742706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21110.3945764891477447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18540.3465394651254944No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8850.16541932396767128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.15794274435331326No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6750.12616728099229163No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT6470.12093367526224102No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6430.12018601730080522No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6220.11626081300326727No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC6090.11383092462860091No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6080.11364401013824196No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5930.1108402927828577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG358.863171E-426.4285721
GTATAGA358.863171E-426.4285721
AAGACGG2000.021.2750025
GGACCGT450.003823174920.5555576
TCTATAC450.003823174920.5555573
GCGTTAT1101.7480488E-920.1818181
TATTATG656.8933194E-519.9230772
CAAGACG2150.019.7906974
CGCAAGA2050.018.9512182
GTTCAAA2550.018.8627451
CGTTATT1306.9303496E-1018.52
GTATATA500.00703008718.51
TAGGAGT500.00703008718.54
AGGCCCG2550.018.13725510
AGACGGA2300.017.6956526
GGTATCA13700.017.6897811
GTACAGG953.5991015E-617.5263161
ACGGACC2500.017.028
CGATGCG1553.9835868E-1016.70967733
TCTAGAT2000.016.6500022
Produced by FastQC (version 0.11.5)