##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631435.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535004
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.887927940725675	34.0	33.0	34.0	31.0	34.0
2	33.053293059491146	34.0	33.0	34.0	31.0	34.0
3	33.164729609498245	34.0	33.0	34.0	31.0	34.0
4	36.480942198563	37.0	37.0	37.0	35.0	37.0
5	36.46017039124941	37.0	37.0	37.0	35.0	37.0
6	36.50628032687606	37.0	37.0	37.0	35.0	37.0
7	36.49768786775426	37.0	37.0	37.0	35.0	37.0
8	36.494585087214304	37.0	37.0	37.0	35.0	37.0
9	38.34939925682799	39.0	39.0	39.0	37.0	39.0
10	38.31867799119259	39.0	39.0	39.0	37.0	39.0
11	38.36535801601483	39.0	39.0	39.0	37.0	39.0
12	38.157916576324666	39.0	39.0	39.0	37.0	39.0
13	38.284717497439274	39.0	39.0	39.0	37.0	39.0
14	39.819790132410226	41.0	40.0	41.0	38.0	41.0
15	39.813593917054824	41.0	40.0	41.0	38.0	41.0
16	39.785448333096575	41.0	40.0	41.0	38.0	41.0
17	39.79380341081562	41.0	40.0	41.0	38.0	41.0
18	39.783911895985824	41.0	40.0	41.0	38.0	41.0
19	39.798347302076245	41.0	40.0	41.0	38.0	41.0
20	39.76794566021936	41.0	40.0	41.0	38.0	41.0
21	39.74058698626553	41.0	40.0	41.0	38.0	41.0
22	39.67966407727793	41.0	40.0	41.0	37.0	41.0
23	39.61774678320162	41.0	40.0	41.0	37.0	41.0
24	39.589247930856594	41.0	40.0	41.0	37.0	41.0
25	39.58787224020755	41.0	40.0	41.0	37.0	41.0
26	39.50337941398569	41.0	39.0	41.0	37.0	41.0
27	39.430994908449286	41.0	39.0	41.0	36.0	41.0
28	39.34077315309792	41.0	39.0	41.0	36.0	41.0
29	39.26007656017525	41.0	39.0	41.0	36.0	41.0
30	39.19726207654522	41.0	39.0	41.0	35.0	41.0
31	39.128341096515165	41.0	39.0	41.0	35.0	41.0
32	39.04939215407735	41.0	39.0	41.0	35.0	41.0
33	38.93729392677438	40.0	39.0	41.0	35.0	41.0
34	38.894421350120744	40.0	39.0	41.0	35.0	41.0
35	38.779130623322445	40.0	38.0	41.0	35.0	41.0
36	38.713220835732066	40.0	38.0	41.0	35.0	41.0
37	38.62549999626171	40.0	38.0	41.0	35.0	41.0
38	38.52089330173232	40.0	38.0	41.0	35.0	41.0
39	38.42086040478202	40.0	38.0	41.0	35.0	41.0
40	38.31349485237494	40.0	38.0	41.0	35.0	41.0
41	38.20279661460475	40.0	37.0	41.0	35.0	41.0
42	38.05632855081458	40.0	37.0	41.0	34.0	41.0
43	37.16550904292304	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	0.0
15	2.0
16	2.0
17	3.0
18	15.0
19	18.0
20	43.0
21	57.0
22	130.0
23	231.0
24	334.0
25	537.0
26	796.0
27	1141.0
28	1680.0
29	2430.0
30	3092.0
31	3933.0
32	5308.0
33	7223.0
34	10791.0
35	15926.0
36	25411.0
37	51150.0
38	106425.0
39	298323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83948531225935	18.17163983820682	12.427196806005188	25.561678043528644
2	19.21294046399653	20.451996620586012	33.593393694252754	26.7416692211647
3	19.912187572429367	22.451981667426786	29.337911492250523	28.297919267893324
4	14.043072575158316	15.697639643815748	33.962175983730965	36.297111797294974
5	15.68848083378816	35.58964045128635	32.26293635187774	16.45894236304775
6	34.01525969899291	34.36161224962804	16.358756196215353	15.2643718551637
7	29.80033794139857	30.332296580960143	20.58078070444333	19.28658477319796
8	28.888755971917966	31.149860561790195	19.701908770775546	20.259474695516293
9	27.430636032627792	14.038586627389702	19.580414352042226	38.95036298794028
10	18.80341081562007	25.290838946998527	30.29510059737871	25.61064964000269
11	37.29766506418643	21.531240887918596	20.118541169785647	21.052552878109324
12	23.324685422912726	25.529715665677266	27.221665632406488	23.92393327900352
13	30.891544736114124	18.246031805369682	24.817010713938586	26.04541274457761
14	23.75496257971903	20.22134413948307	24.037390374651405	31.986302906146495
15	26.687650933450964	26.599988037472617	21.351616062683643	25.360744966392772
16	26.037562335982535	24.951028403525953	23.605804816412586	25.40560444407892
17	24.769721347877773	25.729714170361344	24.225613266442867	25.274951215318016
18	25.11887761586829	23.264872785997863	26.174944486396363	25.441305111737485
19	25.177942594821722	24.81458082556392	25.555509865346803	24.451966714267556
20	25.684480863694475	23.265994272940016	25.53607075834947	25.513454105016038
21	27.584466658193207	23.869728076799426	24.752899043745465	23.792906221261898
22	26.871014048493098	23.60038429619218	24.579255482202004	24.949346173112723
23	25.24242809399556	23.47945062092994	25.260184970579658	26.017936314494843
24	24.59570395735359	24.81644997046751	25.347660952067645	25.240185120111253
25	24.960561042534263	23.721131056964058	25.26878303713617	26.04952486336551
26	25.127101853444085	24.762431682753775	25.40840816143431	24.70205830236783
27	25.75345231063693	23.668608085173197	24.970654425013645	25.60728517917623
28	24.456265747545814	24.248977577737737	26.082795642649405	25.211961032067048
29	24.161688510740106	24.402060545341715	26.38615786050198	25.050093083416197
30	23.642253142032583	25.282240880442014	26.53419413686627	24.541311840659137
31	24.992710334876	24.833646103580534	24.82355272110115	25.350090840442313
32	24.196080776966152	24.196641520437232	25.484856187991117	26.1224215146055
33	23.663374479443146	23.818513506441075	26.41755949488228	26.1005525192335
34	25.02672877212133	23.91159692263983	25.993263601767463	25.06841070347138
35	24.778880157905363	23.79085016186795	26.61942714446995	24.81084253575674
36	23.196088253545767	24.608227228207642	26.382793399675514	25.812891118571073
37	24.121128066332215	23.553281844621722	26.826902228768386	25.49868786027768
38	23.30412482897324	22.737213179714544	27.929136978415116	26.029525012897096
39	23.780943693878925	22.411981966489968	28.008575636817667	25.79849870281344
40	23.018706402195125	22.940389230734723	28.55025382987791	25.490650537192245
41	21.989555218278742	23.11795799657573	29.42949959252641	25.462987192619117
42	22.76842789960449	23.14263070930311	29.340902124096267	24.748039266996138
43	21.414606245934607	22.298524870842087	29.510807395832554	26.776061487390752
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	79.0
2	94.0
3	237.5
4	381.0
5	381.0
6	506.5
7	632.0
8	598.5
9	565.0
10	795.0
11	1025.0
12	1025.0
13	1819.0
14	2613.0
15	3866.5
16	5120.0
17	4623.5
18	4127.0
19	4127.0
20	4683.5
21	5240.0
22	4304.0
23	3368.0
24	3890.0
25	4412.0
26	4412.0
27	5131.5
28	5851.0
29	6933.0
30	8015.0
31	9289.0
32	10563.0
33	10563.0
34	12404.0
35	14245.0
36	16907.0
37	19569.0
38	22174.5
39	24780.0
40	24780.0
41	26597.5
42	28415.0
43	29872.5
44	31330.0
45	33410.0
46	35490.0
47	35490.0
48	38065.5
49	40641.0
50	40448.5
51	40256.0
52	40017.5
53	39779.0
54	39779.0
55	38552.5
56	37326.0
57	35883.0
58	34440.0
59	33384.0
60	32328.0
61	32328.0
62	29763.0
63	27198.0
64	25007.0
65	22816.0
66	20390.5
67	17965.0
68	17965.0
69	15353.5
70	12742.0
71	11024.0
72	9306.0
73	7086.5
74	4867.0
75	4867.0
76	3910.0
77	2953.0
78	2438.0
79	1923.0
80	1768.5
81	1614.0
82	1614.0
83	1310.5
84	1007.0
85	939.5
86	872.0
87	791.0
88	710.0
89	710.0
90	508.0
91	306.0
92	172.0
93	38.0
94	25.0
95	12.0
96	12.0
97	8.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	535004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.660668110144435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.99690173636202	57.01926658753605
2	5.061604807163984	6.3432707785282805
3	1.34498975028605	2.5283386906266068
4	0.597797551681558	1.4983357593190008
5	0.37492353250550337	1.1746479518505148
6	0.24065864241746743	0.9047898792215313
7	0.1726468644015897	0.7572717509367586
8	0.12597942149088254	0.631516377499855
9	0.10205188760596996	0.5755175513362302
>10	0.7723163800629015	9.751559609108783
>50	0.10820320454770987	4.73819314757684
>100	0.09744266334499023	11.42350671982411
>500	0.003586846503496571	1.4376776885717775
>1k	8.967116258741428E-4	1.2161075080636468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2528	0.47251983162742706	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2111	0.3945764891477447	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1854	0.3465394651254944	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	885	0.16541932396767128	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.15794274435331326	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	675	0.12616728099229163	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	647	0.12093367526224102	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	643	0.12018601730080522	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	622	0.11626081300326727	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	609	0.11383092462860091	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	608	0.11364401013824196	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	593	0.1108402927828577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.869144903589506E-4	0.0
3	0.0	0.0	1.869144903589506E-4	1.869144903589506E-4	0.0
4	0.0	0.0	1.869144903589506E-4	1.869144903589506E-4	0.0
5	0.0	1.869144903589506E-4	1.869144903589506E-4	1.869144903589506E-4	0.0
6	0.0	1.869144903589506E-4	1.869144903589506E-4	1.869144903589506E-4	0.0
7	0.0	1.869144903589506E-4	1.869144903589506E-4	1.869144903589506E-4	0.0
8	0.0	1.869144903589506E-4	1.869144903589506E-4	1.869144903589506E-4	0.0
9	0.0	1.869144903589506E-4	1.869144903589506E-4	1.869144903589506E-4	0.0
10	0.0	1.869144903589506E-4	1.869144903589506E-4	1.869144903589506E-4	0.0
11	0.0	1.869144903589506E-4	1.869144903589506E-4	1.869144903589506E-4	0.0
12	0.0	1.869144903589506E-4	1.869144903589506E-4	7.476579614358024E-4	0.0
13	0.0	1.869144903589506E-4	1.869144903589506E-4	7.476579614358024E-4	0.0
14	0.0	1.869144903589506E-4	1.869144903589506E-4	7.476579614358024E-4	0.0
15	0.0	1.869144903589506E-4	1.869144903589506E-4	0.0011214869421537035	0.0
16	0.0	1.869144903589506E-4	1.869144903589506E-4	0.0018691449035895058	0.0
17	0.0	1.869144903589506E-4	1.869144903589506E-4	0.0029906318457432095	0.0
18	0.0	1.869144903589506E-4	1.869144903589506E-4	0.0029906318457432095	0.0
19	0.0	1.869144903589506E-4	1.869144903589506E-4	0.0037382898071790117	0.0
20	0.0	1.869144903589506E-4	1.869144903589506E-4	0.004485947768614814	0.0
21	0.0	1.869144903589506E-4	1.869144903589506E-4	0.005794349201127468	0.0
22	0.0	1.869144903589506E-4	1.869144903589506E-4	0.007476579614358023	0.0
23	0.0	1.869144903589506E-4	1.869144903589506E-4	0.013644757796203394	0.0
24	0.0	1.869144903589506E-4	1.869144903589506E-4	0.020934422920202467	0.0
25	0.0	1.869144903589506E-4	1.869144903589506E-4	0.023364311294868823	0.0
26	0.0	1.869144903589506E-4	1.869144903589506E-4	0.033831522754970055	0.0
27	0.0	1.869144903589506E-4	1.869144903589506E-4	0.07495271063393918	0.0
28	0.0	1.869144903589506E-4	1.869144903589506E-4	0.2069143408273583	0.0
29	0.0	1.869144903589506E-4	1.869144903589506E-4	0.39738020650312894	0.0
30	0.0	1.869144903589506E-4	1.869144903589506E-4	0.6631726117935567	0.0
31	0.0	1.869144903589506E-4	1.869144903589506E-4	1.2927006153225022	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATATG	35	8.863171E-4	26.428572	1
GTATAGA	35	8.863171E-4	26.428572	1
AAGACGG	200	0.0	21.275002	5
GGACCGT	45	0.0038231749	20.555557	6
TCTATAC	45	0.0038231749	20.555557	3
GCGTTAT	110	1.7480488E-9	20.181818	1
TATTATG	65	6.8933194E-5	19.923077	2
CAAGACG	215	0.0	19.790697	4
CGCAAGA	205	0.0	18.951218	2
GTTCAAA	255	0.0	18.862745	1
CGTTATT	130	6.9303496E-10	18.5	2
GTATATA	50	0.007030087	18.5	1
TAGGAGT	50	0.007030087	18.5	4
AGGCCCG	255	0.0	18.137255	10
AGACGGA	230	0.0	17.695652	6
GGTATCA	1370	0.0	17.689781	1
GTACAGG	95	3.5991015E-6	17.526316	1
ACGGACC	250	0.0	17.02	8
CGATGCG	155	3.9835868E-10	16.709677	33
TCTAGAT	200	0.0	16.650002	2
>>END_MODULE