FastQCFastQC Report
Fri 10 Feb 2017
ERR1631434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851015
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15660.1840155578926341No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13690.16086672972861818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.16063171624471953No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC13210.15522640611505084No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11440.13442771279002133No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11340.13325264537052814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11160.13113752401544038No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT10490.123264572304836No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10140.11915183633660982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10010.11762424869126867No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC9670.1136290194649918No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT9460.11116137788405611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT250.00549555329.59999815
GCGTAAA250.00549555329.59999814
CCTAGGT358.8674965E-426.428573
TATAACT509.087471E-625.8999984
ATAACAC400.001930777423.1253
TATCGTC400.001930777423.12511
GGTATCA8600.022.8023241
ATATAAC656.8994384E-519.9230773
GCAGTCG2750.019.509099
GCCTTAT2400.019.27083226
CAGGACA4800.018.8854164
TACTTTA701.2187255E-418.57
ATGCTAG701.2187255E-418.515
TGCATCA4100.018.514
CTTTAGA609.2341314E-418.52
TAGATCC500.007033420318.4999985
GTCTTAA500.007033420318.4999981
CACTTAG500.007033420318.4999981
CTCTATG3950.018.2658231
ATACACA2250.018.08888837
Produced by FastQC (version 0.11.5)