##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631434.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	851015
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83781249449187	34.0	31.0	34.0	31.0	34.0
2	33.01302914754734	34.0	33.0	34.0	31.0	34.0
3	33.122004900031136	34.0	33.0	34.0	31.0	34.0
4	36.462709822976095	37.0	37.0	37.0	35.0	37.0
5	36.438132112829976	37.0	37.0	37.0	35.0	37.0
6	36.47320317503217	37.0	37.0	37.0	35.0	37.0
7	36.46007884702385	37.0	37.0	37.0	35.0	37.0
8	36.442204896505935	37.0	37.0	37.0	35.0	37.0
9	38.29191142341792	39.0	39.0	39.0	37.0	39.0
10	38.25145032696251	39.0	39.0	39.0	37.0	39.0
11	38.31206382966223	39.0	39.0	39.0	37.0	39.0
12	38.08418888033701	39.0	39.0	39.0	37.0	39.0
13	38.21213844644336	39.0	39.0	39.0	37.0	39.0
14	39.744691926699296	41.0	40.0	41.0	38.0	41.0
15	39.745353489656466	41.0	40.0	41.0	38.0	41.0
16	39.73147476836483	41.0	40.0	41.0	38.0	41.0
17	39.71669829556471	41.0	40.0	41.0	38.0	41.0
18	39.67488822171172	41.0	40.0	41.0	37.0	41.0
19	39.683324030716264	41.0	40.0	41.0	37.0	41.0
20	39.65825396732137	41.0	40.0	41.0	37.0	41.0
21	39.63241188463188	41.0	40.0	41.0	37.0	41.0
22	39.57992397313796	41.0	40.0	41.0	37.0	41.0
23	39.51645740674371	41.0	39.0	41.0	37.0	41.0
24	39.47868721467894	41.0	39.0	41.0	37.0	41.0
25	39.463611099686844	41.0	39.0	41.0	37.0	41.0
26	39.38688037226136	41.0	39.0	41.0	36.0	41.0
27	39.323305699664516	41.0	39.0	41.0	36.0	41.0
28	39.2215624871477	41.0	39.0	41.0	36.0	41.0
29	39.14871653261106	41.0	39.0	41.0	35.0	41.0
30	39.09396661633461	41.0	39.0	41.0	35.0	41.0
31	39.05496142841196	40.0	39.0	41.0	35.0	41.0
32	38.986543127911965	40.0	39.0	41.0	35.0	41.0
33	38.8850983825197	40.0	38.0	41.0	35.0	41.0
34	38.83744587345699	40.0	38.0	41.0	35.0	41.0
35	38.713349353419154	40.0	38.0	41.0	35.0	41.0
36	38.67064975352961	40.0	38.0	41.0	35.0	41.0
37	38.60079787077784	40.0	38.0	41.0	35.0	41.0
38	38.50871018724699	40.0	38.0	41.0	35.0	41.0
39	38.430240360040656	40.0	38.0	41.0	35.0	41.0
40	38.31560430779716	40.0	38.0	41.0	34.0	41.0
41	38.22098552904473	40.0	37.0	41.0	34.0	41.0
42	38.107660852041384	40.0	37.0	41.0	34.0	41.0
43	37.145622580095534	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	3.0
15	3.0
16	3.0
17	3.0
18	9.0
19	26.0
20	71.0
21	137.0
22	220.0
23	362.0
24	639.0
25	922.0
26	1366.0
27	1897.0
28	2719.0
29	3598.0
30	5023.0
31	6554.0
32	8634.0
33	11876.0
34	18342.0
35	27708.0
36	42856.0
37	79754.0
38	186375.0
39	451913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21615952715287	17.223080674253683	12.006486372155603	27.55427342643784
2	19.819274630881946	18.59473687302809	33.47778828810303	28.108200207986933
3	20.434069904760786	20.490355634154508	28.06766038201442	31.007914079070286
4	15.062366703289602	14.750973837123905	32.30213333490009	37.8845261246864
5	17.030604630940697	34.048988560718676	31.668889502535208	17.251517305805418
6	36.43414040880595	32.77204279595542	15.203609807112684	15.590206988125942
7	31.88827458975459	28.471883574320078	19.455473757806853	20.18436807811848
8	29.247310564443634	31.232469462935438	18.626698706838305	20.893521265782624
9	27.961551794034182	13.848404552211182	18.136343072683793	40.05370058107084
10	19.83889825678748	23.950929184561964	29.21828639918215	26.991886159468397
11	39.39566282615465	20.085662414881053	19.11411667244408	21.404558086520215
12	24.76983366920677	23.65093447236535	25.75959295664589	25.81963890178199
13	31.73445826454293	16.70793111754787	23.61850261158734	27.93910800632186
14	24.359970153287545	18.914237704388288	22.24813898697438	34.477653155349785
15	27.849450362214533	24.960899631616364	19.870155050146003	27.3194949560231
16	27.840519849826386	23.499585788734628	21.373888826871443	27.286005534567547
17	26.554056038965236	24.176424622362706	22.51875701368366	26.750762324988397
18	26.996351415662474	21.679053835713823	24.046814685992608	27.277780062631095
19	27.160860854391522	23.094540049235324	22.947891635282573	26.79670746109058
20	27.1901200331369	22.167411855255196	22.94377889931435	27.698689212293555
21	29.875736620388594	22.41852376280089	22.131219778734803	25.57451983807571
22	28.98092278044453	22.092795074117376	22.064240935823694	26.8620412096144
23	27.135714411614366	22.124169374217846	22.83943291246335	27.900683301704436
24	26.681550853980244	23.089252245847604	22.919690017214737	27.309506882957407
25	26.979665458305668	21.974465784974413	22.973743118511425	28.072125638208494
26	27.354746978607896	23.308284812841137	23.27479539138558	26.062172817165386
27	28.371650323437308	21.95437213210108	22.411590865025882	27.262386679435735
28	26.43090897340235	22.24778646674853	23.922727566494128	27.398576993354993
29	26.14054981404558	22.523339776619682	24.242228397854326	27.093882011480407
30	25.4031950083136	23.297826712807648	24.40239008713125	26.8965881917475
31	26.814450979124928	22.839667925947253	22.67950623667033	27.66637485825749
32	25.940788352731737	22.37234361321481	23.372913520913265	28.313954513140192
33	25.900248526759224	22.126519509056834	23.94105861823822	28.032173345945722
34	27.223726961334403	22.075639089792777	23.913209520396233	26.787424428476587
35	27.158980746520335	21.81430409569749	24.570777248344626	26.455937909437555
36	25.50049059064764	22.786672385328107	23.934478240689057	27.778358783335193
37	25.923632368407134	21.462958937269025	25.005317180073206	27.60809151425063
38	25.400727366732667	20.81866947116091	26.022338031644566	27.75826513046186
39	25.44808258373824	20.619260530072914	26.271217311093224	27.66143957509562
40	25.067478246564395	20.805508716062583	27.05040451695916	27.07660852041386
41	23.557516612515645	21.079769451772297	28.211488634160386	27.15122530155168
42	24.442812406361817	21.43275970458805	27.610559155831567	26.513868733218573
43	22.961992444316493	20.148881042049787	28.253203527552394	28.635922986081326
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	34.0
2	36.0
3	84.5
4	133.0
5	133.0
6	178.0
7	223.0
8	226.0
9	229.0
10	360.5
11	492.0
12	492.0
13	829.5
14	1167.0
15	1887.0
16	2607.0
17	2400.0
18	2193.0
19	2193.0
20	2451.5
21	2710.0
22	2345.5
23	1981.0
24	2459.5
25	2938.0
26	2938.0
27	3604.5
28	4271.0
29	5597.0
30	6923.0
31	8656.5
32	10390.0
33	10390.0
34	13216.0
35	16042.0
36	20283.0
37	24524.0
38	28919.5
39	33315.0
40	33315.0
41	36199.0
42	39083.0
43	42289.5
44	45496.0
45	48724.5
46	51953.0
47	51953.0
48	55968.5
49	59984.0
50	62223.5
51	64463.0
52	67607.0
53	70751.0
54	70751.0
55	69407.5
56	68064.0
57	65971.5
58	63879.0
59	61429.0
60	58979.0
61	58979.0
62	56143.5
63	53308.0
64	48908.0
65	44508.0
66	39917.5
67	35327.0
68	35327.0
69	30206.5
70	25086.0
71	22329.0
72	19572.0
73	16867.5
74	14163.0
75	14163.0
76	12710.5
77	11258.0
78	9011.0
79	6764.0
80	4980.5
81	3197.0
82	3197.0
83	2510.0
84	1823.0
85	1661.5
86	1500.0
87	1244.5
88	989.0
89	989.0
90	760.0
91	531.0
92	311.0
93	91.0
94	58.5
95	26.0
96	26.0
97	16.5
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	851015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.38150237704768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.79967539240499	45.99078905271276
2	6.4429062805224575	6.749782212965652
3	1.863413431484193	2.9282518527213552
4	0.8956069084492516	1.876529416153392
5	0.541512350679876	1.41826152421693
6	0.38028351356233514	1.1951893061770504
7	0.2564462711726216	0.9403128674109584
8	0.20806883265480675	0.8719166441837836
9	0.16093214470880732	0.7586880768546996
>10	1.1380268587391555	11.82532408621144
>50	0.16016870110921363	5.944534779667753
>100	0.13854194171651776	14.110744150432469
>500	0.012164658296477468	3.964111145300898
>1k	0.0022527144993476794	1.4255648849907765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1566	0.1840155578926341	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1369	0.16086672972861818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1367	0.16063171624471953	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1321	0.15522640611505084	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1144	0.13442771279002133	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1134	0.13325264537052814	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1116	0.13113752401544038	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1049	0.123264572304836	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1014	0.11915183633660982	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1001	0.11762424869126867	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	967	0.1136290194649918	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	946	0.11116137788405611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.350134838986387E-4	0.0	0.0	0.0	0.0
6	3.52520225847958E-4	0.0	0.0	0.0	0.0
7	3.52520225847958E-4	0.0	0.0	0.0	0.0
8	3.52520225847958E-4	0.0	0.0	0.0	0.0
9	3.52520225847958E-4	0.0	0.0	0.0	0.0
10	4.700269677972774E-4	0.0	0.0	0.0	0.0
11	5.875337097465967E-4	0.0	0.0	1.1750674194931935E-4	0.0
12	5.875337097465967E-4	0.0	0.0	1.1750674194931935E-4	0.0
13	5.875337097465967E-4	0.0	0.0	1.1750674194931935E-4	0.0
14	5.875337097465967E-4	0.0	0.0	4.700269677972774E-4	0.0
15	5.875337097465967E-4	0.0	0.0	7.05040451695916E-4	0.0
16	5.875337097465967E-4	0.0	0.0	9.400539355945548E-4	0.0
17	5.875337097465967E-4	0.0	0.0	0.0012925741614425127	0.0
18	5.875337097465967E-4	0.0	0.0	0.0015275876453411513	0.0
19	7.05040451695916E-4	0.0	0.0	0.0017626011292397902	0.0
20	7.05040451695916E-4	0.0	0.0	0.002115121355087748	0.0
21	7.05040451695916E-4	0.0	0.0	0.0030551752906823027	0.0
22	7.05040451695916E-4	0.0	0.0	0.004817776419922093	0.0
23	7.05040451695916E-4	0.0	0.0	0.007050404516959161	0.0
24	7.05040451695916E-4	0.0	0.0	0.010105579807641464	0.0
25	7.05040451695916E-4	0.0	0.0	0.01245571464662785	0.0
26	7.05040451695916E-4	0.0	0.0	0.019271105679688372	0.0
27	8.225471936452353E-4	0.0	0.0	0.05052789903820732	0.0
28	9.400539355945548E-4	0.0	0.0	0.19517869837781943	0.0
29	9.400539355945548E-4	0.0	0.0	0.40739587433829016	0.0
30	9.400539355945548E-4	0.0	0.0	0.7030428370827776	0.0
31	9.400539355945548E-4	0.0	0.0	1.3966851348096097	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAAT	25	0.005495553	29.599998	15
GCGTAAA	25	0.005495553	29.599998	14
CCTAGGT	35	8.8674965E-4	26.42857	3
TATAACT	50	9.087471E-6	25.899998	4
ATAACAC	40	0.0019307774	23.125	3
TATCGTC	40	0.0019307774	23.125	11
GGTATCA	860	0.0	22.802324	1
ATATAAC	65	6.8994384E-5	19.923077	3
GCAGTCG	275	0.0	19.50909	9
GCCTTAT	240	0.0	19.270832	26
CAGGACA	480	0.0	18.885416	4
TACTTTA	70	1.2187255E-4	18.5	7
ATGCTAG	70	1.2187255E-4	18.5	15
TGCATCA	410	0.0	18.5	14
CTTTAGA	60	9.2341314E-4	18.5	2
TAGATCC	50	0.0070334203	18.499998	5
GTCTTAA	50	0.0070334203	18.499998	1
CACTTAG	50	0.0070334203	18.499998	1
CTCTATG	395	0.0	18.265823	1
ATACACA	225	0.0	18.088888	37
>>END_MODULE