FastQCFastQC Report
Fri 10 Feb 2017
ERR1631433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5754
Sequences flagged as poor quality0
Sequence length43
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC120.20855057351407716No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT110.19117135905457075No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA110.19117135905457075No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC90.15641293013555788No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG90.15641293013555788No Hit
TCACTGTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATC80.13903371567605144No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC80.13903371567605144No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG80.13903371567605144No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT60.10427528675703858RNA PCR Primer, Index 29 (95% over 23bp)
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC60.10427528675703858No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT60.10427528675703858No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC60.10427528675703858No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCC60.10427528675703858No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)