##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631433.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5754
Sequences flagged as poor quality	0
Sequence length	43
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82290580465763	34.0	31.0	34.0	31.0	34.0
2	32.98557525199861	34.0	33.0	34.0	31.0	34.0
3	33.090198123044836	34.0	33.0	34.0	31.0	34.0
4	36.4224887035106	37.0	37.0	37.0	35.0	37.0
5	36.40180743830379	37.0	37.0	37.0	35.0	37.0
6	36.48418491484185	37.0	37.0	37.0	35.0	37.0
7	36.41501564129301	37.0	37.0	37.0	35.0	37.0
8	36.42440041710115	37.0	37.0	37.0	35.0	37.0
9	38.28258602711158	39.0	39.0	39.0	37.0	39.0
10	38.25182481751825	39.0	39.0	39.0	37.0	39.0
11	38.31664928745221	39.0	39.0	39.0	37.0	39.0
12	38.10427528675704	39.0	39.0	39.0	37.0	39.0
13	38.2076816127911	39.0	39.0	39.0	37.0	39.0
14	39.737052485227665	41.0	40.0	41.0	38.0	41.0
15	39.738790406673616	41.0	40.0	41.0	38.0	41.0
16	39.74539450816823	41.0	40.0	41.0	38.0	41.0
17	39.67101147028154	41.0	40.0	41.0	37.0	41.0
18	39.66805700382343	41.0	40.0	41.0	37.0	41.0
19	39.6442474800139	41.0	40.0	41.0	37.0	41.0
20	39.63573166492875	41.0	40.0	41.0	37.0	41.0
21	39.58950295446646	41.0	40.0	41.0	37.0	41.0
22	39.57090719499479	41.0	39.0	41.0	37.0	41.0
23	39.50208550573514	41.0	39.0	41.0	37.0	41.0
24	39.49200556134863	41.0	39.0	41.0	37.0	41.0
25	39.42683350712548	41.0	39.0	41.0	36.0	41.0
26	39.30848105665624	41.0	39.0	41.0	36.0	41.0
27	39.279631560653456	41.0	39.0	41.0	36.0	41.0
28	39.125999304831424	40.0	39.0	41.0	35.0	41.0
29	39.04136253041362	40.0	39.0	41.0	35.0	41.0
30	38.98696558915537	40.0	39.0	41.0	35.0	41.0
31	38.99061522419186	40.0	39.0	41.0	35.0	41.0
32	38.88338547097671	40.0	38.0	41.0	35.0	41.0
33	38.78762599930483	40.0	38.0	41.0	35.0	41.0
34	38.66475495307612	40.0	38.0	41.0	35.0	41.0
35	38.6442474800139	40.0	38.0	41.0	35.0	41.0
36	38.59263121306917	40.0	38.0	41.0	35.0	41.0
37	38.47480013903372	40.0	38.0	41.0	34.0	41.0
38	38.38269030239833	40.0	38.0	41.0	34.0	41.0
39	38.29405630865485	40.0	37.0	41.0	34.0	41.0
40	38.24574209245742	40.0	37.0	41.0	34.0	41.0
41	38.09680222453945	40.0	37.0	41.0	34.0	41.0
42	37.98262078554049	40.0	37.0	41.0	34.0	41.0
43	36.99061522419186	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	0.0
23	0.0
24	4.0
25	5.0
26	10.0
27	10.0
28	19.0
29	24.0
30	41.0
31	41.0
32	65.0
33	95.0
34	125.0
35	194.0
36	338.0
37	563.0
38	1267.0
39	2951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.056308654848806	15.849843587069865	11.087938825165104	29.00590893291623
2	21.8804310045186	18.126520681265205	28.866875217240178	31.126173096976018
3	20.820298922488703	18.647897115050398	27.89363920750782	32.638164754953074
4	15.658672228015295	14.285714285714285	30.118178658324645	39.93743482794578
5	18.022245394508168	32.32533889468196	32.064650677789366	17.58776503302051
6	39.50295446645812	31.821341675356273	13.399374348279459	15.276329509906153
7	33.90684741049704	26.642335766423358	18.196037539103234	21.25477928397636
8	30.72645116440737	29.770594369134518	17.0316301703163	22.471324296141816
9	27.441779631560653	13.034410844629823	16.127911018421965	43.39589850538756
10	19.412582551268684	22.332290580465763	29.75321515467501	28.501911713590545
11	43.10045185957595	17.153284671532848	17.083767813694823	22.662495655196384
12	24.92179353493222	21.63712200208551	24.800139033715677	28.640945429266594
13	34.84532499131039	15.18943343760862	20.281543274244004	29.683698296836987
14	24.50469238790407	17.153284671532848	19.67327076816128	38.66875217240181
15	30.448383733055266	22.45394508168231	17.257559958289885	29.840111226972542
16	29.927007299270077	20.92457420924574	20.020855057351408	29.127563434132774
17	28.206465067778936	21.306916927354884	19.933958985053877	30.552659019812307
18	28.988529718456725	20.97671185262426	21.02884949600278	29.00590893291623
19	29.44038929440389	21.532846715328464	19.91657977059437	29.11018421967327
20	30.13555787278415	19.48209940910671	20.194647201946474	30.18769551616267
21	31.960375391032326	20.333680917622523	18.943343760862007	28.76259993048314
22	30.396246089676747	20.333680917622523	20.090371915189433	29.179701077511293
23	30.62217587765033	20.420576989920054	19.238790406673616	29.718456725756
24	28.832116788321166	21.237400069516855	20.472714633298576	29.457768508863396
25	29.092805005213762	19.951338199513383	19.447340980187697	31.508515815085158
26	29.214459506430305	21.515467500868958	19.760166840458812	29.509906152241918
27	30.62217587765033	20.45533541883907	20.437956204379564	28.48453249913104
28	29.127563434132774	19.360444907890166	20.716023635731666	30.795968022245397
29	27.806743135210287	20.15988877302746	21.793534932221064	30.23983315954119
30	27.40702120264164	22.123740006951685	21.306916927354884	29.162321863051787
31	28.415015641293014	21.063607924921794	20.611748348974626	29.90962808481057
32	27.94577685088634	20.78554049356969	20.15988877302746	31.108793882516512
33	27.21584984358707	21.22002085505735	22.10636079249218	29.457768508863396
34	28.60618700034758	20.50747306221759	22.05422314911366	28.832116788321166
35	28.849496002780672	20.125130344108445	22.21063607924922	28.81473757386166
36	26.659714980882864	21.619742787626002	22.228015293708726	29.492526937782408
37	26.607577337504345	19.708029197080293	23.149113660062564	30.5352798053528
38	25.286757038581857	20.559610705596107	24.852276677094196	29.30135557872784
39	27.598192561696212	18.3872088981578	24.34827945776851	29.66631908237748
40	26.92040319777546	19.464720194647203	25.18248175182482	28.43239485575252
41	24.887035106013208	19.42996176572819	26.485922836287802	29.1970802919708
42	25.443169968717417	18.769551616266945	28.48453249913104	27.302745915884604
43	24.000695168578382	17.535627389641988	27.963156065345846	30.500521376433788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	2.5
16	4.0
17	3.0
18	2.0
19	2.0
20	1.5
21	1.0
22	0.5
23	0.0
24	2.5
25	5.0
26	5.0
27	7.0
28	9.0
29	12.0
30	15.0
31	19.0
32	23.0
33	23.0
34	29.5
35	36.0
36	50.0
37	64.0
38	99.0
39	134.0
40	134.0
41	143.5
42	153.0
43	179.0
44	205.0
45	241.5
46	278.0
47	278.0
48	323.5
49	369.0
50	396.0
51	423.0
52	438.5
53	454.0
54	454.0
55	469.5
56	485.0
57	491.0
58	497.0
59	517.0
60	537.0
61	537.0
62	522.0
63	507.0
64	476.5
65	446.0
66	404.0
67	362.0
68	362.0
69	305.5
70	249.0
71	222.0
72	195.0
73	151.5
74	108.0
75	108.0
76	90.5
77	73.0
78	59.0
79	45.0
80	41.5
81	38.0
82	38.0
83	27.0
84	16.0
85	14.0
86	12.0
87	8.5
88	5.0
89	5.0
90	4.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5754.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.78762599930484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.4860460760442	88.59923531456378
2	3.1466566772803897	5.839416058394161
3	0.6742835737029407	1.8769551616266946
4	0.2622213897733658	0.9732360097323601
5	0.1873009926952613	0.8689607229753215
6	0.09365049634763065	0.5213764337851928
7	0.0	0.0
8	0.05619029780857839	0.4171011470281543
9	0.037460198539052254	0.31282586027111575
>10	0.05619029780857839	0.5908932916232186
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	12	0.20855057351407716	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	11	0.19117135905457075	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	11	0.19117135905457075	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	9	0.15641293013555788	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	9	0.15641293013555788	No Hit
TCACTGTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATC	8	0.13903371567605144	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	8	0.13903371567605144	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	8	0.13903371567605144	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	6	0.10427528675703858	RNA PCR Primer, Index 29 (95% over 23bp)
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	6	0.10427528675703858	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	6	0.10427528675703858	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	6	0.10427528675703858	No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCC	6	0.10427528675703858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.01737921445950643	0.0
23	0.0	0.0	0.0	0.01737921445950643	0.0
24	0.0	0.0	0.0	0.01737921445950643	0.0
25	0.0	0.0	0.0	0.01737921445950643	0.0
26	0.0	0.0	0.0	0.03475842891901286	0.0
27	0.0	0.0	0.0	0.05213764337851929	0.0
28	0.0	0.0	0.0	0.19117135905457072	0.0
29	0.0	0.0	0.0	0.2954466458116093	0.0
30	0.0	0.0	0.0	0.48661800486618007	0.0
31	0.0	0.0	0.0	1.094890510948905	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE