FastQCFastQC Report
Fri 10 Feb 2017
ERR1631432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271557
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18440.14501905931075051No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14880.11702188733969457No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT14360.1129324127821246No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14140.11120225046930651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA601.3375939E-624.6666681
GGTATCA7800.023.7179491
GTCTTAA400.001931299523.1251
ATAATAC656.902868E-519.9230773
TATACTG656.902868E-519.9230775
TATATAG500.007035283418.52
GTATAGA1501.2732926E-1118.51
GACGGAC3050.016.9836067
CTTATAC9200.016.89130437
GTTCAAA4700.016.5319161
AGACGGA3400.016.323536
TAAGGCT2500.016.27999934
AGACTCG803.382919E-416.187531
ATATAGG803.382919E-416.18753
TCGTTTA3000.016.03333330
TACCGTC1504.671165E-916.0333337
ACGGACC3250.015.938468
AGACGTA700.002592696815.8571425
TGTACTG3300.015.696975
CGCTTAT1659.731593E-1015.6969726
Produced by FastQC (version 0.11.5)