##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631432.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1271557
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86952845999039	34.0	33.0	34.0	31.0	34.0
2	33.04502747419109	34.0	33.0	34.0	31.0	34.0
3	33.164208918672145	34.0	33.0	34.0	31.0	34.0
4	36.483757314851005	37.0	37.0	37.0	35.0	37.0
5	36.460614821042235	37.0	37.0	37.0	35.0	37.0
6	36.503811468931396	37.0	37.0	37.0	35.0	37.0
7	36.48903588277993	37.0	37.0	37.0	35.0	37.0
8	36.473601262074766	37.0	37.0	37.0	35.0	37.0
9	38.33866275754842	39.0	39.0	39.0	37.0	39.0
10	38.28600448112039	39.0	39.0	39.0	37.0	39.0
11	38.3537552779781	39.0	39.0	39.0	37.0	39.0
12	38.119579381813004	39.0	39.0	39.0	37.0	39.0
13	38.25452181852642	39.0	39.0	39.0	37.0	39.0
14	39.79710937063773	41.0	40.0	41.0	38.0	41.0
15	39.80797400352481	41.0	40.0	41.0	38.0	41.0
16	39.798322057131536	41.0	40.0	41.0	38.0	41.0
17	39.77748618426072	41.0	40.0	41.0	38.0	41.0
18	39.7565189763416	41.0	40.0	41.0	38.0	41.0
19	39.766152048236926	41.0	40.0	41.0	38.0	41.0
20	39.734779486881045	41.0	40.0	41.0	38.0	41.0
21	39.70871301876361	41.0	40.0	41.0	38.0	41.0
22	39.67064236994488	41.0	40.0	41.0	37.0	41.0
23	39.61206457909476	41.0	40.0	41.0	37.0	41.0
24	39.58658636616369	41.0	40.0	41.0	37.0	41.0
25	39.55541827853568	41.0	40.0	41.0	37.0	41.0
26	39.487738261045315	41.0	39.0	41.0	37.0	41.0
27	39.421226889553516	41.0	39.0	41.0	37.0	41.0
28	39.34453587216303	41.0	39.0	41.0	36.0	41.0
29	39.269273025118025	41.0	39.0	41.0	36.0	41.0
30	39.21507097204451	41.0	39.0	41.0	36.0	41.0
31	39.16820402073993	41.0	39.0	41.0	35.0	41.0
32	39.09948511942445	41.0	39.0	41.0	35.0	41.0
33	39.00589356198739	40.0	39.0	41.0	35.0	41.0
34	38.96352581913355	40.0	39.0	41.0	35.0	41.0
35	38.86360029475674	40.0	38.0	41.0	35.0	41.0
36	38.8097710130179	40.0	38.0	41.0	35.0	41.0
37	38.74488520766273	40.0	38.0	41.0	35.0	41.0
38	38.653704080902386	40.0	38.0	41.0	35.0	41.0
39	38.565730832357495	40.0	38.0	41.0	35.0	41.0
40	38.47648591451268	40.0	38.0	41.0	35.0	41.0
41	38.38936909631263	40.0	37.0	41.0	34.0	41.0
42	38.28137551049619	40.0	37.0	41.0	34.0	41.0
43	37.329348192806144	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	1.0
16	1.0
17	6.0
18	26.0
19	36.0
20	102.0
21	158.0
22	281.0
23	468.0
24	730.0
25	1160.0
26	1624.0
27	2559.0
28	3508.0
29	4710.0
30	6519.0
31	8739.0
32	11929.0
33	16209.0
34	24948.0
35	37333.0
36	61407.0
37	114657.0
38	277554.0
39	696889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67566691858878	17.23304578559986	11.35867287113358	27.732614424677777
2	20.573517349202593	19.025493941679375	31.617772541852236	28.783216167265802
3	20.405848892342224	20.465696779617428	28.178288507711414	30.950165820328934
4	15.012382457097873	14.752071672760245	33.00221696707265	37.23332890306923
5	16.97910514432306	33.83104335865399	32.46421513152772	16.725636365495216
6	36.871095829758325	33.17625556699385	14.791786762213569	15.16086184103426
7	32.22458765120243	27.901541181402013	19.67155227803394	20.202318889361624
8	29.86621913134842	30.210442787857723	18.49315445552185	21.430183625272008
9	28.00676650751795	13.180297855306526	17.630983117548013	41.181952519627515
10	19.182230918472392	23.911000450628638	30.160268080785997	26.746500550112973
11	40.07189610847174	19.571753370080934	18.966668423043558	21.389682098403767
12	24.67793421765599	23.18983734114947	25.72916511017595	26.403063331018583
13	32.913664114152965	16.71478352916936	22.296837656510878	28.0747147001668
14	24.69193280364152	18.265480823903292	21.63025330362697	35.41233306882822
15	28.35099016402725	24.47188761494766	19.40581507553338	27.771307145491708
16	28.572922802516914	22.881396587018905	21.13613467583443	27.409545934629747
17	27.13987654505461	23.353730898418238	22.073568074415853	27.4328244821113
18	27.366527808033776	21.708661113894227	23.26242551454634	27.66238556352566
19	27.895564257048644	22.420544261877367	22.41794901840814	27.265942462665848
20	27.88581243310367	21.746410109810256	22.342686957800556	28.025090499285522
21	29.162672219963397	22.299983406170547	21.714559394506104	26.822784979359948
22	29.181782649145887	21.778654043821867	21.546340431455295	27.49322287557695
23	27.57540558543581	21.649442376550954	22.430846592012784	28.344305446000455
24	27.298658259126405	22.523960781938992	21.923201240683664	28.25417971825093
25	27.85679289249322	21.504030098532745	22.173052407402892	28.466124601571146
26	27.77634034494718	22.547868479352477	22.160783983730184	27.51500719197016
27	28.134877162407978	21.80798815939828	21.79878684164375	28.25834783654999
28	27.249112701986622	22.010417149997995	22.962635571979863	27.77783457603552
29	26.74972494351413	22.39640063323941	23.310476840597786	27.543397582648677
30	26.327329407962054	23.005810986058826	23.12660777299012	27.540251832989004
31	27.788215549912433	22.67936081512665	21.885452244767638	27.646971390193283
32	26.81051655568724	22.33018260290337	22.394827758409573	28.46447308299982
33	26.241371798511587	21.94750215680461	23.225934818494174	28.585191226189625
34	27.71311077678783	21.61696251131487	23.06683852945641	27.6030881824409
35	27.388783986875932	21.998620588774234	23.5965827721447	27.01601265220513
36	26.250573116266118	22.80165183314629	23.155784601083553	27.791990449504034
37	26.86446616235057	21.499154186560258	23.899518464370846	27.736861186718333
38	25.93143681329268	21.383311955342936	24.821852264585857	27.863398966778526
39	26.18160255497787	21.138179413113214	24.91606746689295	27.76415056501596
40	25.9160226399603	21.202116774945992	25.50227791597231	27.3795826691214
41	24.80706724118541	21.140224150392	26.519927930875298	27.53278067754729
42	25.116373076472385	21.254965369228433	26.606671977740675	27.021989576558504
43	23.97965643695092	20.36747074649426	27.218441642804848	28.43443117374998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	8.0
2	9.0
3	37.5
4	66.0
5	66.0
6	79.5
7	93.0
8	86.5
9	80.0
10	126.0
11	172.0
12	172.0
13	319.0
14	466.0
15	742.5
16	1019.0
17	995.0
18	971.0
19	971.0
20	1181.5
21	1392.0
22	1393.5
23	1395.0
24	1828.5
25	2262.0
26	2262.0
27	3065.5
28	3869.0
29	5644.5
30	7420.0
31	9625.5
32	11831.0
33	11831.0
34	15874.0
35	19917.0
36	25719.0
37	31521.0
38	38355.5
39	45190.0
40	45190.0
41	51218.5
42	57247.0
43	63214.0
44	69181.0
45	74405.5
46	79630.0
47	79630.0
48	85994.0
49	92358.0
50	96474.0
51	100590.0
52	102454.5
53	104319.0
54	104319.0
55	103413.0
56	102507.0
57	99468.0
58	96429.0
59	96614.0
60	96799.0
61	96799.0
62	91412.0
63	86025.0
64	80251.5
65	74478.0
66	66950.0
67	59422.0
68	59422.0
69	51044.0
70	42666.0
71	37532.0
72	32398.0
73	25353.5
74	18309.0
75	18309.0
76	14996.5
77	11684.0
78	9736.5
79	7789.0
80	6667.5
81	5546.0
82	5546.0
83	4273.5
84	3001.0
85	2390.5
86	1780.0
87	1421.5
88	1063.0
89	1063.0
90	784.0
91	505.0
92	302.5
93	100.0
94	70.5
95	41.0
96	41.0
97	23.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1271557.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.927062268260826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.35475875047445	47.432132110479515
2	7.650144020345835	8.403998739333977
3	2.1175161693537756	3.4892682746443193
4	0.9783454778505386	2.149505719270717
5	0.5708033280801332	1.5676274972193993
6	0.3601262014383538	1.186840457450202
7	0.2600052790550702	0.999692830691406
8	0.19385763973093786	0.8518424518943438
9	0.15297275422103282	0.7562109596801425
>10	1.1016049574194289	12.244794351536582
>50	0.140044248761047	5.376999983451116
>100	0.10932064383516399	11.322340097316697
>500	0.009349786482594217	3.369245391016852
>1k	0.0011507429517039036	0.8495011360147545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1844	0.14501905931075051	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1488	0.11702188733969457	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1436	0.1129324127821246	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1414	0.11120225046930651	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.864374149173021E-5	0.0	0.0	0.0
3	0.0	7.864374149173021E-5	0.0	0.0	7.864374149173021E-5
4	0.0	7.864374149173021E-5	0.0	0.0	7.864374149173021E-5
5	0.0	7.864374149173021E-5	0.0	0.0	7.864374149173021E-5
6	0.0	7.864374149173021E-5	0.0	0.0	7.864374149173021E-5
7	0.0	7.864374149173021E-5	0.0	0.0	7.864374149173021E-5
8	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
9	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
10	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
11	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
12	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
13	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
14	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
15	0.0	1.5728748298346042E-4	0.0	0.0	1.5728748298346042E-4
16	0.0	2.3593122447519065E-4	0.0	1.5728748298346042E-4	2.3593122447519065E-4
17	0.0	2.3593122447519065E-4	0.0	3.1457496596692085E-4	2.3593122447519065E-4
18	0.0	2.3593122447519065E-4	0.0	3.932187074586511E-4	2.3593122447519065E-4
19	0.0	2.3593122447519065E-4	0.0	4.718624489503813E-4	3.1457496596692085E-4
20	0.0	2.3593122447519065E-4	0.0	6.291499319338417E-4	3.1457496596692085E-4
21	0.0	2.3593122447519065E-4	0.0	9.437248979007626E-4	3.932187074586511E-4
22	0.0	2.3593122447519065E-4	0.0	0.0012582998638676834	3.932187074586511E-4
23	0.0	3.1457496596692085E-4	0.0	0.002595243469227097	4.718624489503813E-4
24	0.0	3.1457496596692085E-4	0.0	0.0042467620405534316	4.718624489503813E-4
25	0.0	3.1457496596692085E-4	0.0	0.005740993128896306	4.718624489503813E-4
26	0.0	3.1457496596692085E-4	0.0	0.009358605237515897	5.505061904421115E-4
27	0.0	3.1457496596692085E-4	0.0	0.02563785972630405	6.291499319338417E-4
28	0.0	4.718624489503813E-4	0.0	0.08894607162714688	6.291499319338417E-4
29	0.0	6.291499319338417E-4	0.0	0.1717579314179388	6.291499319338417E-4
30	0.0	6.291499319338417E-4	0.0	0.28036493841801824	6.291499319338417E-4
31	0.0	6.291499319338417E-4	0.0	0.5825928369707375	6.291499319338417E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATATA	60	1.3375939E-6	24.666668	1
GGTATCA	780	0.0	23.717949	1
GTCTTAA	40	0.0019312995	23.125	1
ATAATAC	65	6.902868E-5	19.923077	3
TATACTG	65	6.902868E-5	19.923077	5
TATATAG	50	0.0070352834	18.5	2
GTATAGA	150	1.2732926E-11	18.5	1
GACGGAC	305	0.0	16.983606	7
CTTATAC	920	0.0	16.891304	37
GTTCAAA	470	0.0	16.531916	1
AGACGGA	340	0.0	16.32353	6
TAAGGCT	250	0.0	16.279999	34
AGACTCG	80	3.382919E-4	16.1875	31
ATATAGG	80	3.382919E-4	16.1875	3
TCGTTTA	300	0.0	16.033333	30
TACCGTC	150	4.671165E-9	16.033333	7
ACGGACC	325	0.0	15.93846	8
AGACGTA	70	0.0025926968	15.857142	5
TGTACTG	330	0.0	15.69697	5
CGCTTAT	165	9.731593E-10	15.69697	26
>>END_MODULE