FastQCFastQC Report
Fri 10 Feb 2017
ERR1631431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8200
Sequences flagged as poor quality0
Sequence length43
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA540.6585365853658537No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT480.5853658536585366TruSeq Adapter, Index 6 (95% over 21bp)
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA310.3780487804878049No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA260.3170731707317073No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA260.3170731707317073No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC240.2926829268292683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT240.2926829268292683No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT220.2682926829268293No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT220.2682926829268293No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC210.25609756097560976No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA210.25609756097560976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200.24390243902439024No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA160.1951219512195122No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA160.1951219512195122No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT160.1951219512195122No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT150.1829268292682927No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT150.1829268292682927No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC150.1829268292682927No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC150.1829268292682927No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG140.17073170731707318No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC140.17073170731707318No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC130.15853658536585366RNA PCR Primer, Index 6 (95% over 22bp)
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG130.15853658536585366No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT130.15853658536585366No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT120.14634146341463414No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC110.13414634146341464No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA110.13414634146341464ABI Solid3 Adapter B (100% over 21bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG110.13414634146341464No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT110.13414634146341464No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT100.12195121951219512No Hit
GGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT100.12195121951219512No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA100.12195121951219512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100.12195121951219512No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT90.10975609756097562No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT90.10975609756097562No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA90.10975609756097562No Hit
TCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCC90.10975609756097562ABI Solid3 Adapter B (100% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATGC303.2999774E-430.83333234
TATGCCC303.2999774E-430.83333236
CCTCGCC303.2999774E-430.83333222
TTGATAT303.2999774E-430.83333232
ATATGCC303.2999774E-430.83333235
TCCTCGC250.005187941729.59999821
GCCTTAT250.005187941729.59999826
CGCCTTA250.005187941729.59999825
CTCGCCT250.005187941729.59999823
CAGTCGG405.263302E-527.75000210
GGGCAGT405.263302E-527.7500027
TCGGTGA405.263302E-527.75000213
TTATTGA405.263302E-527.75000229
TGATTCC405.263302E-527.75000217
TATTGAT405.263302E-527.75000230
GCAGTCG405.263302E-527.7500029
CTTATTG405.263302E-527.75000228
AGTCGGT405.263302E-527.75000211
CGGTGAT405.263302E-527.75000214
ATTCCTC405.263302E-527.75000219
Produced by FastQC (version 0.11.5)