FastQCFastQC Report
Fri 10 Feb 2017
ERR1631430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187708
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.3436188121976687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5020.2674366569352398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4240.22588275406482408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.11773605813284463No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1930.10281927248705436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGA250.005484780829.5999988
CAGTCGG250.005484780829.59999810
TAGAACT358.841661E-426.428574
ACTCTAA358.841661E-426.4285710
GTCTAGT358.841661E-426.428571
TCTAGTG358.841661E-426.428572
GGTATCA2650.025.1320761
CGAGCCG551.891686E-523.54545415
GTATAAA400.001925205823.1251
TCTGGAG502.690449E-422.1999993
GTTCAAA855.122638E-821.7647061
AGGCCCG603.7049496E-521.58333210
GATAACG450.003814071620.55555511
TGAACAC450.003814071620.5555555
CACTCTA450.003814071620.5555559
ACGGACC450.003814071620.5555558
GTGTAGA450.003814071620.5555551
CGGACCA450.003814071620.5555559
AAGACGG555.121092E-420.1818185
CAAGACG555.121092E-420.1818184
Produced by FastQC (version 0.11.5)