FastQCFastQC Report
Fri 10 Feb 2017
ERR1631428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711733
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10520.14780823707766816No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8110.11394722459124419No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8100.11380672246474449No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7960.11183969269374892No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC7320.10284755659776909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTA851.9736035E-923.9411775
CGCTTAT1155.456968E-1222.5217426
CCGCTTA1201.0913936E-1121.58333225
CAATATA603.72372E-521.5833322
CTTATAC14300.020.95804237
CGTAACG450.003824398220.55555526
GGTATCA6950.020.49641
GGGTATC555.1404507E-420.1818181
TAGCGAA555.1404507E-420.18181810
CGTCCTA555.1404507E-420.18181833
CCTAAAC759.257643E-619.7333343
GCAGTCG3350.018.776129
GATCCGT609.232105E-418.529
TCGGGTA609.232105E-418.525
GTCTAGG1205.164111E-918.51
GTAGCGA609.232105E-418.59
GCTTATT1409.458745E-1118.527
ATAAGGT500.007032313418.54
TAGATAA1800.018.54
TATACAC3400.018.537
Produced by FastQC (version 0.11.5)